A single-cell CRISPRi platform for characterizing candidate genes relevant to metabolic disorders in human adipocytes

CROP-Seq combines gene silencing using CRISPR interference with single-cell RNA sequencing. Here, we applied CROP-Seq to study adipogenesis and adipocyte biology. Human preadipocyte SGBS cell line expressing KRAB-dCas9 was transduced with a sgRNA library. Following selection, individual cells were c...

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Veröffentlicht in:	American Journal of Physiology: Cell Physiology Jg. 325; H. 3; S. C648
Hauptverfasser:	Bielczyk-Maczynska, Ewa, Sharma, Disha, Blencowe, Montgomery, Saliba Gustafsson, Peter, Gloudemans, Michael J, Yang, Xia, Carcamo-Orive, Ivan, Wabitsch, Martin, Svensson, Katrin J, Park, Chong Y, Quertermous, Thomas, Knowles, Joshua W, Li, Jiehan
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.09.2023
Schlagworte:	Adipocytes - metabolism Adipogenesis - genetics Cell Differentiation - genetics Cell Line Humans Metabolic Diseases - metabolism RNA, Guide, CRISPR-Cas Systems single-cell RNA-Seq SGBS adipogenesis CRISPRi adipocyte
ISSN:	1522-1563, 1522-1563
Online-Zugang:	Weitere Angaben
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