A eukaryotic transcriptional repressor with carboxypeptidase activity

Adipocyte differentiation involves the transcriptional activation of several genes in triglyceride metabolism, including the adipose P2 (aP2 or 422) gene that encodes the adipocyte lipid-binding protein ALBP. Within the mouse aP2 promoter region, the AE-1 sequence functions as either a positive or a...

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Vydané v:Nature (London) Ročník 378; číslo 6552; s. 92 - 96
Hlavní autori: GONG-PING HE, MUISE, A, WU LI, A, HYO-SUNG RO
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing 02.11.1995
Nature Publishing Group
Predmet:
3T3 Cells
Adipocytes - cytology
Adipocytes - metabolism
Amino Acid Sequence
Animals
Base Sequence
Biochemistry
Biological and medical sciences
Carboxypeptidases - genetics
Carboxypeptidases - metabolism
Cell Differentiation
Chloramphenicol O-Acetyltransferase - genetics
Cloning
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA, Complementary
Escherichia coli
Frameshift Mutation
Fundamental and applied biological sciences. Psychology
Intercellular Signaling Peptides and Proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
Metabolism
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Proteins
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Rodents
Sequence Deletion
Transcription. Transcription factor. Splicing. Rna processing
Adipocyte
Nucleotide sequence
Enzyme
Rodentia
Homology
3T3-Cell line
Transcription repressor
Carboxypeptidase
Cell differentiation
Gene expression
Vertebrata
Mammalia
Mouse
Enzymatic activity
Complementary DNA
Gene
Fibroblast
ISSN:0028-0836, 1476-4687
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Shrnutí:Adipocyte differentiation involves the transcriptional activation of several genes in triglyceride metabolism, including the adipose P2 (aP2 or 422) gene that encodes the adipocyte lipid-binding protein ALBP. Within the mouse aP2 promoter region, the AE-1 sequence functions as either a positive or a negative element in the regulation of aP2 gene expression. The AE-1 sequence is the binding site for the positive murine (3T3) adipocyte factor C/EBP-alpha, several human preadipocyte factors, and a 3T3 preadipocyte factor(s) that has been implicated as a repressor of aP2 gene expression. Here we report the cloning of new complementary DNAs that encode the 3T3 preadipocyte factor (termed AEBP1) and demonstrate that AEBP1 expression is abolished during adipocyte differentiation. Furthermore, we show that an activity of a carboxypeptidase associated with AEBP1 is important in the transcriptional repression function of AEBP1. Thus AEBP1 might represent a new type of transcription factor that regulates transcription by cleavage of factors involved in transcription.
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ISSN:0028-0836
1476-4687
DOI:10.1038/378092a0