Quantification of ribonucleotide reductase expression in wild-type and hydroxyurea-resistant cell lines employing in situ reverse transcriptase polymerase chain reaction and a computerized image analysis system

Ribonucleotide reductase (RR) is the enzyme responsible for converting nucleoside diphosphates to deoxynucleoside diphosphates, ensuring a balanced supply of deoxyribonucleotides for DNA synthesis. Expression of RR is tightly regulated, but it is affected by exogenous agents, such as hydroxyurea (HU...

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Bibliographic Details

Published in:	Analytical biochemistry Vol. 267; no. 1; p. 24
Main Authors:	Wadler, S, Zhang, H, Cammer, M, Hu, X P
Format:	Journal Article
Language:	English
Published:	United States 01.02.1999
Subjects:	Base Sequence Cell Line DNA - analysis DNA - genetics DNA Primers - genetics Drug Resistance - genetics Gene Expression Regulation, Enzymologic Genetic Variation Humans Hydroxyurea - pharmacology Image Processing, Computer-Assisted - methods Reverse Transcriptase Polymerase Chain Reaction - methods Ribonucleotide Reductases - genetics RNA, Messenger - analysis RNA, Messenger - genetics
ISSN:	0003-2697
Online Access:	Get full text
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