Quantification of ribonucleotide reductase expression in wild-type and hydroxyurea-resistant cell lines employing in situ reverse transcriptase polymerase chain reaction and a computerized image analysis system

Ribonucleotide reductase (RR) is the enzyme responsible for converting nucleoside diphosphates to deoxynucleoside diphosphates, ensuring a balanced supply of deoxyribonucleotides for DNA synthesis. Expression of RR is tightly regulated, but it is affected by exogenous agents, such as hydroxyurea (HU...

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Veröffentlicht in:	Analytical biochemistry Jg. 267; H. 1; S. 24
Hauptverfasser:	Wadler, S, Zhang, H, Cammer, M, Hu, X P
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.02.1999
Schlagworte:	Base Sequence Cell Line DNA - analysis DNA - genetics DNA Primers - genetics Drug Resistance - genetics Gene Expression Regulation, Enzymologic Genetic Variation Humans Hydroxyurea - pharmacology Image Processing, Computer-Assisted - methods Reverse Transcriptase Polymerase Chain Reaction - methods Ribonucleotide Reductases - genetics RNA, Messenger - analysis RNA, Messenger - genetics
ISSN:	0003-2697
Online-Zugang:	Volltext
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