Recording Lineage History with Cellular Barcodes in the Mammary Epithelium and in Breast Cancer

Lineage tracing methods have extensively advanced our understanding of physiological cell behaviour in vivo and in situ and have vastly contributed to decipher the phylogeny and cellular hierarchies during normal and tumour development. In recent years, increasingly complex systems have been develop...

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Vydáno v:Advances in experimental medicine and biology Ročník 1464; s. 77
Hlavní autoři: Merle, Candice, Fre, Silvia
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 2025
Témata:
Animals
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Lineage
DNA Barcoding, Taxonomic - methods
Female
Humans
Mammary Glands, Human - cytology
Mammary Glands, Human - metabolism
Mammary Glands, Human - pathology
Single-Cell Analysis - methods
Genetic barcodes
Optical barcodes
Cellular barcoding
Mouse genetics
Single-cell lineage tracing
ISSN:0065-2598
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Shrnutí:Lineage tracing methods have extensively advanced our understanding of physiological cell behaviour in vivo and in situ and have vastly contributed to decipher the phylogeny and cellular hierarchies during normal and tumour development. In recent years, increasingly complex systems have been developed to track thousands of cells within a given tissue or even entire organisms. Cellular barcoding comprises all techniques designed to genetically label single cells with unique DNA sequences or with a combination of fluorescent proteins, in order to trace their history and lineage production in space and time. We distinguish these two types of cellular barcoding as genetic or optical barcodes. Furthermore, transcribed cellular barcodes can integrate the lineage information with single-cell profiling of each barcoded cell. This enables the potential identification of specific markers or signalling pathways defining distinct stem cell states during development, but also signals promoting tumour growth and metastasis or conferring therapy resistance.In this chapter, we describe recent advances in cellular barcoding technologies and outline experimental and computational challenges. We discuss the biological questions that can be addressed using single-cell dynamic lineage tracing, with a focus on the study of cellular hierarchies in the mammary epithelium and in breast cancer.
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ISSN:0065-2598
DOI:10.1007/978-3-031-70875-6_5