A Serological Assay for the Detection of Antibodies Generated Against California Serogroup Virus Infection

The California serogroup (CSG) viruses are orthobunyaviruses endemic in North America and responsible for the second most common cause of mosquito-borne viral encephalitis in the United States. As the CSG viruses have been neglected and are poorly studied, there are no commercial diagnostic serologi...

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Veröffentlicht in:Methods in molecular biology (Clifton, N.J.) Jg. 2893; S. 25
Hauptverfasser: Stulberg, Alyssa, Barry, Christina, Robinson, Alyssia, Mueller, Nicole, Bello, Alexander, Wood, Heidi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 2025
Schlagworte:
Animals
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigens, Viral - immunology
Encephalitis Virus, California - immunology
Encephalitis, California - diagnosis
Encephalitis, California - immunology
Encephalitis, California - virology
Enzyme-Linked Immunosorbent Assay - methods
Humans
Immunoglobulin M - blood
Immunoglobulin M - immunology
Neutralization Tests - methods
Serogroup
Serologic Tests - methods
Virus antigen purification
P/N ratio
California serogroup virus
MAC-ELISA
ISSN:1940-6029, 1940-6029
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Zusammenfassung:The California serogroup (CSG) viruses are orthobunyaviruses endemic in North America and responsible for the second most common cause of mosquito-borne viral encephalitis in the United States. As the CSG viruses have been neglected and are poorly studied, there are no commercial diagnostic serological assays or reagents available for detection. Therefore, diagnostic laboratories have had to rely on the development of their own in-house serological assays. To develop serological assays, antigenic materials such as recombinant protein, virus-like particles (VLP), or inactivated virus can be used in the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) to detect CSG virus-specific IgM antibodies. All positive MAC-ELISA results should be confirmed by a well-defined gold standard test method such as the plaque-reduction neutralization test (PRNT).
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content type line 23
ISSN:1940-6029
1940-6029
DOI:10.1007/978-1-0716-4338-9_3