Structural and functional analysis of the promoter region of the mts1 gene

The mts1 gene is specifically expressed in metastatic tumors but not in non-metastatic counterparts. The gene was cloned from both mouse and human sources. The 5'-flanking regions of the mts1 gene from both sources were sequenced, revealing a 135 base pair region of high homology in the vicinit...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Genetika Jg. 28; H. 8; S. 22
Hauptverfasser: Tul'chinskiĭ, E M, Kramerov, D A, Reshetniakh, E Iu, Grigorian, M S, Gural', N I, Lukanidin, E M
Format: Journal Article
Sprache:Russisch
Veröffentlicht: Russia (Federation) 01.08.1992
Schlagworte:
Animals
Base Sequence
Chromosome Mapping
Cloning, Molecular
Enhancer Elements, Genetic - genetics
Gene Expression Regulation - physiology
Humans
Methylation
Mice
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids - genetics
Promoter Regions, Genetic - genetics
Sequence Homology, Nucleic Acid
TATA Box
Transcription, Genetic - genetics
Transfection - genetics
Tumor Cells, Cultured
ISSN:0016-6758
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The mts1 gene is specifically expressed in metastatic tumors but not in non-metastatic counterparts. The gene was cloned from both mouse and human sources. The 5'-flanking regions of the mts1 gene from both sources were sequenced, revealing a 135 base pair region of high homology in the vicinity of the TATA box. The 5' region of the mts1 gene was also observed to have high degree of homology with some known promoter and enhancer sequences. The results of our transient transfection assays, in conjunction with those obtained from in vivo and in vitro footprinting of the promoter region, show no evidence of cis-control elements important for transcriptional regulation of mts1 gene. mts1 was found to be hypermethylated in CSML-0 cells and in the tissue types that do not express the gene or do that only at low levels. The possible role of methylation in progression of the nonmetastatic CSML-0 adenosarcoma cell line towards the metastatic CSML-100 adenosarcoma cell line will be discussed.
Bibliographie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0016-6758