Preparation of Leukocyte–Poor Platelet Concentrates via a Short, Hard Spin of a Pool of Buffy Coats
Background and Objectives: A new method for the preparation of leukocyte–poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PC...
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| Published in: | Vox sanguinis Vol. 78; no. 3; pp. 164 - 170 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Basel, Switzerland
01.01.2000
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| Subjects: | |
| ISSN: | 0042-9007, 1423-0410 |
| Online Access: | Get full text |
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| Summary: | Background and Objectives: A new method for the preparation of leukocyte–poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7–9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet–rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device. Results: A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290×10 9 platelets and less than 5×10 6 leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool. Conclusion: The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte–poor PCs. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0042-9007 1423-0410 |
| DOI: | 10.1159/000031175 |