Preparation of Leukocyte–Poor Platelet Concentrates via a Short, Hard Spin of a Pool of Buffy Coats
Background and Objectives: A new method for the preparation of leukocyte–poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PC...
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| Veröffentlicht in: | Vox sanguinis Jg. 78; H. 3; S. 164 - 170 |
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Basel, Switzerland
01.01.2000
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| Abstract | Background and Objectives: A new method for the preparation of leukocyte–poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7–9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet–rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device. Results: A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290×10 9 platelets and less than 5×10 6 leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool. Conclusion: The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte–poor PCs. |
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| AbstractList | Background and Objectives: A new method for the preparation of leukocyte–poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7–9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet–rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device. Results: A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290×10 9 platelets and less than 5×10 6 leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool. Conclusion: The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte–poor PCs. A new method for the preparation of leukocyte-poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets.BACKGROUND AND OBJECTIVESA new method for the preparation of leukocyte-poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets.The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7-9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet-rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device.MATERIALS AND METHODSThe characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7-9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet-rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device.A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290x10(9) platelets and less than 5x10(6) leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool.RESULTSA short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290x10(9) platelets and less than 5x10(6) leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool.The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte-poor PCs.CONCLUSIONThe preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte-poor PCs. |
| Author | van Delden, C.J. Smit Sibinga, C.Th de Wit, H.J.C. Faber, R.D. |
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| References | Högman CF, Gong J, Eriksson L, Hambraeus A, Johansson CS: White cells protect donor blood against bacterial contamination. Transfusion 1991;31:620-626.190982010.1046/j.1537-2995.1991.31791368338.x van Delden CJ, de Wit HJC, Smit Sibinga CTh: Comparison of blood component preparation systems based on buffy coat removal: Component specifications, efficiency, and process costs. Transfusion 1998;38:860-866.973862710.1046/j.1537-2995.1998.38998409007.x Heaton WAL, Rebulla P, Pappalettera M, Dzik WH: A comparative analysis of different methods for routine blood component preparation. Transfus Med Rev 1997;11:116-129.914017110.1053/tm.1997.0110116 de Wildt-Eggen J, Bins M, van Prooijen HC: Evaluation of storage conditions of platelet concentrates prepared from pooled buffy coats. Vox Sang 1996;70:11-15.8928484 Racz Z, Baroti CL: Storage of platelet concentrates from overnight-stored blood and overnight-stored buffy coat: In vitro studies. Vox Sang 1995;68:160-163.7625072 Pietersz RNI, Dekker WJA, Reesink HW: Comparison of a conventional quadruple-bag system with a 'top-and-bottom' system for blood processing. Vox Sang 1990;59:205-208.2293459 Boeri N, Saleun S, Pelissier E, Saleun JP, Aiach M, Rendu F: Influence of a 12-hour, 22°C holding period for buffy coats on the preparation of platelet concentrates stored in plasma. Transfusion 1994;34:881-886.794066010.1046/j.1537-2995.1994.341095026974.x Eriksson L, Shanwell A, Gulliksson H, Högman CF: Platelet concentrates in an additive solution prepared from pooled buffy coats. Vox Sang 1993;64:133-138.8484245 Rebulla P, Bertolini F, Riccardi D, Smacchia C, Sirchia G: Platelet concentrates prepared from pooled buffy coats and stored in a glucose-free crystalloid medium. The Milan experience. Transfus Sci 1990;11:357-362. Anderson NA, Gray S, Copplestone JA, Chan DC, Hamon M, Prentice AG, Johnson SA, Philips M, von Waeg G, Oakhill A, Abeyasekera S, Pamphilon DH: A prospective randomized study of three types of platelet concentrates in patients with haematological malignancy: Corrected platelet count increments and frequency of nonhaemolytic febrile transfusion reactions. Transfus Med 1997;6: 33-39. Heddle NM: The efficacy of leukodepletion to improve platelet transfusion response: A critical appraisal of clinical studies. Transfus Med Rev 1994;8:15-28.8136604 Pietersz RNI, de Korte D, Reesink HW, Dekker WJA: Storage of whole blood for up to 24 hours at ambient temperature prior to component preparation. Vox Sang 1989;56:145- 150.2499118 Pietersz RNI, Loos JA, Reesink HW: Platelet concentrates stored in plasma for 72 hours at 22°C prepared from buffycoats of citrate-phosphate-dextrose blood collected in a quadruple-bag saline-adenine-glucose-mannitol system. Vox Sang 1985;49:81-85.3929473 Kretschmer V, Biermann E, Loh H: Separation of platelet concentrate from buffy coat (BC) using the bottom and top drainage system. Transfus Sci 1990;11:363-366. Solberg C, Hansen J-B, Little C: Centrifugation of freshly donated blood may yield platelets unstable to storage in the new generation of containers. Vox Sang 1989;56:25-31.2492699 Eriksson L, Högman CF: Platelet concentrates in an additive solution prepared from pooled buffy coats. Vox Sang 1990;59:140-145.2264316 Sanz C, Pereira A, Vila J, Faundez A-Z, Gomez J, Ordinas A: Growth of bacteria in platelet concentrates obtained from whole blood stored for 16 hours at 22°C before component preparation. Transfusion 1997;37:251- 254.912289510.1046/j.1537-2995.1997.37397240204.x |
| References_xml | – reference: Kretschmer V, Biermann E, Loh H: Separation of platelet concentrate from buffy coat (BC) using the bottom and top drainage system. Transfus Sci 1990;11:363-366. – reference: Högman CF, Gong J, Eriksson L, Hambraeus A, Johansson CS: White cells protect donor blood against bacterial contamination. Transfusion 1991;31:620-626.190982010.1046/j.1537-2995.1991.31791368338.x – reference: Pietersz RNI, Loos JA, Reesink HW: Platelet concentrates stored in plasma for 72 hours at 22°C prepared from buffycoats of citrate-phosphate-dextrose blood collected in a quadruple-bag saline-adenine-glucose-mannitol system. Vox Sang 1985;49:81-85.3929473 – reference: Pietersz RNI, de Korte D, Reesink HW, Dekker WJA: Storage of whole blood for up to 24 hours at ambient temperature prior to component preparation. Vox Sang 1989;56:145- 150.2499118 – reference: Eriksson L, Högman CF: Platelet concentrates in an additive solution prepared from pooled buffy coats. Vox Sang 1990;59:140-145.2264316 – reference: Eriksson L, Shanwell A, Gulliksson H, Högman CF: Platelet concentrates in an additive solution prepared from pooled buffy coats. Vox Sang 1993;64:133-138.8484245 – reference: Rebulla P, Bertolini F, Riccardi D, Smacchia C, Sirchia G: Platelet concentrates prepared from pooled buffy coats and stored in a glucose-free crystalloid medium. The Milan experience. Transfus Sci 1990;11:357-362. – reference: Heaton WAL, Rebulla P, Pappalettera M, Dzik WH: A comparative analysis of different methods for routine blood component preparation. Transfus Med Rev 1997;11:116-129.914017110.1053/tm.1997.0110116 – reference: Racz Z, Baroti CL: Storage of platelet concentrates from overnight-stored blood and overnight-stored buffy coat: In vitro studies. Vox Sang 1995;68:160-163.7625072 – reference: Anderson NA, Gray S, Copplestone JA, Chan DC, Hamon M, Prentice AG, Johnson SA, Philips M, von Waeg G, Oakhill A, Abeyasekera S, Pamphilon DH: A prospective randomized study of three types of platelet concentrates in patients with haematological malignancy: Corrected platelet count increments and frequency of nonhaemolytic febrile transfusion reactions. Transfus Med 1997;6: 33-39. – reference: Pietersz RNI, Dekker WJA, Reesink HW: Comparison of a conventional quadruple-bag system with a 'top-and-bottom' system for blood processing. Vox Sang 1990;59:205-208.2293459 – reference: Sanz C, Pereira A, Vila J, Faundez A-Z, Gomez J, Ordinas A: Growth of bacteria in platelet concentrates obtained from whole blood stored for 16 hours at 22°C before component preparation. Transfusion 1997;37:251- 254.912289510.1046/j.1537-2995.1997.37397240204.x – reference: Heddle NM: The efficacy of leukodepletion to improve platelet transfusion response: A critical appraisal of clinical studies. Transfus Med Rev 1994;8:15-28.8136604 – reference: de Wildt-Eggen J, Bins M, van Prooijen HC: Evaluation of storage conditions of platelet concentrates prepared from pooled buffy coats. Vox Sang 1996;70:11-15.8928484 – reference: Solberg C, Hansen J-B, Little C: Centrifugation of freshly donated blood may yield platelets unstable to storage in the new generation of containers. Vox Sang 1989;56:25-31.2492699 – reference: van Delden CJ, de Wit HJC, Smit Sibinga CTh: Comparison of blood component preparation systems based on buffy coat removal: Component specifications, efficiency, and process costs. Transfusion 1998;38:860-866.973862710.1046/j.1537-2995.1998.38998409007.x – reference: Boeri N, Saleun S, Pelissier E, Saleun JP, Aiach M, Rendu F: Influence of a 12-hour, 22°C holding period for buffy coats on the preparation of platelet concentrates stored in plasma. Transfusion 1994;34:881-886.794066010.1046/j.1537-2995.1994.341095026974.x |
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| Title | Preparation of Leukocyte–Poor Platelet Concentrates via a Short, Hard Spin of a Pool of Buffy Coats |
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