Fine-scale diversity and distribution of ectomycorrhizal fungal mycelium in a Scots pine forest

Ectomycorrhizal (ECM) mycelium is a key component of the ectomycorrhizal symbiosis, yet we know little regarding the fine-scale diversity and distribution of mycelium in ECM fungal communities. We collected four 20 × 20 × 2-cm3 (800-cm3) slices of Scots pine (Pinus sylvestris) forest soil and divide...

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Published in:The New phytologist Vol. 201; no. 4; pp. 1423 - 1430
Main Authors: Anderson, Ian C., Genney, David R., Alexander, Ian J.
Format: Journal Article
Language:English
Published: England New Phytologist Trust 01.03.2014
Wiley Subscription Services, Inc
Subjects:
Agaricales
Biodiversity
Chi-Square Distribution
Coniferous forests
Cubes
Digital rights management
Distribution
ectomycorrhiza
ectomycorrhizae
ectomycorrhizal (ECM) mycelium
Ectomycorrhizas
Evergreen trees
Forest soils
Fungi
internal transcribed spacers
Mineral soils
Mosses
Mycelium
Mycelium - physiology
Mycorrhizae - physiology
Pine
Pine trees
Pinus sylvestris
Pinus sylvestris - microbiology
Pinus sylvestris - physiology
Polymorphism
Principal Component Analysis
Restriction fragment length polymorphism
Scotland
Scots pine (Pinus sylvestris)
Soil
Soil fungi
spatial ecology
Species Specificity
Substrates
Symbiosis
terminal restriction fragment length polymorphism (T‐RFLP)
Trees - microbiology
Trees - physiology
Tricholoma
ectomycorrhiza
Scots pine (Pinus sylvestris)
ectomycorrhizal (ECM) mycelium
spatial ecology
terminal restriction fragment length polymorphism (T-RFLP)
ISSN:0028-646X, 1469-8137, 1469-8137
Online Access:Get full text
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Summary:Ectomycorrhizal (ECM) mycelium is a key component of the ectomycorrhizal symbiosis, yet we know little regarding the fine-scale diversity and distribution of mycelium in ECM fungal communities. We collected four 20 × 20 × 2-cm3 (800-cm3) slices of Scots pine (Pinus sylvestris) forest soil and divided each into 100 2 × 2 × 2-cm3 (8-cm3) cubes. The presence of mycelium of ECM fungi was determined using an internal transcribed spacer (ITS) database terminal restriction fragment length polymorphism (T-RFLP) approach. As expected, many more ECM fungi were detected as mycelium than as ectomycorrhizas in a cube or slice. More surprisingly, up to one-quarter of the 43 species previously detected as ectomycorrhizas over an area of 400 m2 could be detected in a single 8-cm3 cube, and up to three-quarters in a single 800-cm3 slice. ECM mycelium frequency decreased markedly with depth and there were distinct ‘hotspots’ of mycelium in the moss/F1 layer. Our data demonstrate a high diversity of ECM mycelium in a small (8-cm3) volume of substrate, and indicate that the spatial scale at which ECM species are distributed as mycelium may be very different from the spatial scale at which they are distributed as tips.
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ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.12637