Concurrent overactivation of the cytosolic glutamine synthetase and the GABA shunt in the ABA-deficient sitiens mutant of tomato leads to resistance against Botrytis cinerea

Deficiency of abscisic acid (ABA) in the sitiens mutant of tomato (Solanum lycopersicum) culminates in increased resistance to Botrytis cinerea through a rapid epidermal hypersensitive response (HR) and associated phenylpropanoid pathway-derived cell wall fortifications. This study focused on unders...

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Vydáno v:	The New phytologist Ročník 199; číslo 2; s. 490 - 504
Hlavní autoři:	Seifi, Hamed Soren, Curvers, Katrien, Vleesschauwer, David, Delaere, Ilse, Aziz, Aziz, Höfte, Monica
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England New Phytologist Trust 01.07.2013 Wiley Subscription Services, Inc
Témata:	Abscisic acid abscisic acid (ABA) Abscisic Acid - metabolism Abscisic Acid - pharmacology Botrytis Botrytis - physiology Botrytis cinerea carbon Cell viability Cell walls Cellular senescence central C : N metabolism Cytosol - drug effects Cytosol - enzymology Disease Resistance - drug effects Enzyme Activation - drug effects Enzymes GABA shunt gamma-Aminobutyric Acid - metabolism gamma-Aminobutyric Acid - pharmacology Gene Expression Regulation, Plant - drug effects Gene silencing genes Glutamate-ammonia ligase Glutamate-Ammonia Ligase - metabolism glutamic acid Glutamine GS/GOGAT cycle Host-Pathogen Interactions - drug effects Hypersensitive response Infections Inoculation Leaves Lycopersicon esculentum Lycopersicon esculentum - cytology Lycopersicon esculentum - enzymology Lycopersicon esculentum - immunology Lycopersicon esculentum - microbiology Metabolic Networks and Pathways - drug effects Metabolism Models, Biological Mutants Mutation - genetics necrotrophic interaction Nitrogen Pathogens Phenylalanine Ammonia-Lyase - metabolism Plant diseases Plants Propanols - metabolism Replenishment RNA, Messenger - genetics RNA, Messenger - metabolism Senescence Shunt resistance Solanum Solanum lycopersicum Tomatoes Transcription transcription (genetics) Tricarboxylic acid cycle γ-Aminobutyric acid
ISSN:	0028-646X, 1469-8137, 1469-8137
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Shrnutí:	Deficiency of abscisic acid (ABA) in the sitiens mutant of tomato (Solanum lycopersicum) culminates in increased resistance to Botrytis cinerea through a rapid epidermal hypersensitive response (HR) and associated phenylpropanoid pathway-derived cell wall fortifications. This study focused on understanding the role of primary carbon : nitrogen (C : N) metabolism in the resistance response of sitiens to B. cinerea. How alterations in C : N metabolism are linked with the HR-mediated epidermal arrest of the pathogen has been also investigated. Temporal alterations in the c-aminobutyric acid (GABA) shunt, glutamine synthetase/glutamate synthase (GS/GOGAT) cycle and phenylpropanoid pathway were transcriptionally, enzymatically and metabolically monitored in both wild-type and sitiens plants. Virus-induced gene silencing, microscopic analyses and pharmacological assays were used to further confirm the data. Our results on the sitiens–B. cinerea interaction favor a model in which cell viability in the cells surrounding the invaded tissue is maintained by a constant replenishment of the tricarboxylic acid (TCA) cycle through overactivation of the GS/GOGAT cycle and the GABA shunt, resulting in resistance through both tightly controlling the defense-associated HR and slowing down the pathogen-induced senescence. Collectively, this study shows that maintaining cell viability via alterations in host C : N metabolism plays a vital role in the resistance response against necrotrophic pathogens.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 14 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0028-646X 1469-8137 1469-8137
DOI:	10.1111/nph.12283