Exosomal and Non‐Exosomal Urinary miRNAs in Prostate Cancer Detection and Prognosis
BACKGROUND MicroRNAs (miRNAs) are non‐coding small RNAs, involved in post‐transcriptional regulation of many target genes. METHODS Five miRNAs that have been consistently found deregulated in PCa (miR‐21, miR‐141, miR‐214, miR‐375, and let‐7c) were analyzed in urinary pellets from 60 prostate cancer...
Uložené v:
| Vydané v: | The Prostate Ročník 77; číslo 6; s. 573 - 583 |
|---|---|
| Hlavní autori: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
United States
Wiley Subscription Services, Inc
01.05.2017
|
| Predmet: | |
| ISSN: | 0270-4137, 1097-0045, 1097-0045 |
| On-line prístup: | Získať plný text |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | BACKGROUND
MicroRNAs (miRNAs) are non‐coding small RNAs, involved in post‐transcriptional regulation of many target genes.
METHODS
Five miRNAs that have been consistently found deregulated in PCa (miR‐21, miR‐141, miR‐214, miR‐375, and let‐7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT‐PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs.
RESULTS
Significant upregulation of miR‐21, miR‐141, and miR‐375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR‐214 was found significantly downregulated. Regarding urinary exosomes, miR‐21 and miR‐375 were also significantly upregulated in PCa but no differences were found for miR‐141. Significant differences were found for let‐7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR‐21 and miR‐375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR‐141 resulted significantly correlated with Gleason score in urinary pellets and let‐7c with clinical stage in urinary exosomes. Additionally, miR‐21, miR‐141, and miR‐214 were found significantly deregulated in intermediate/high‐risk PCa versus low‐risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR‐21, miR‐375, and let‐7c.
CONCLUSIONS
These findings suggest that the analysis of miRNAs—especially miRNA‐21 and miR‐375‐ in urine could be useful as biomarkers in PCa. Prostate 77: 573–583, 2017. © 2016 Wiley Periodicals, Inc. |
|---|---|
| AbstractList | BACKGROUND MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. METHODS Five miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs. RESULTS Significant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c. CONCLUSIONS These findings suggest that the analysis of miRNAs-especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes.BACKGROUNDMicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes.Five miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs.METHODSFive miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs.Significant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c.RESULTSSignificant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c.These findings suggest that the analysis of miRNAs-especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. © 2016 Wiley Periodicals, Inc.CONCLUSIONSThese findings suggest that the analysis of miRNAs-especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. © 2016 Wiley Periodicals, Inc. MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. Five miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs. Significant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c. These findings suggest that the analysis of miRNAs-especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. © 2016 Wiley Periodicals, Inc. BACKGROUND MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. METHODS Five miRNAs that have been consistently found deregulated in PCa (miR-21, miR-141, miR-214, miR-375, and let-7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT-PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs. RESULTS Significant upregulation of miR-21, miR-141, and miR-375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR-214 was found significantly downregulated. Regarding urinary exosomes, miR-21 and miR-375 were also significantly upregulated in PCa but no differences were found for miR-141. Significant differences were found for let-7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR-21 and miR-375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR-141 resulted significantly correlated with Gleason score in urinary pellets and let-7c with clinical stage in urinary exosomes. Additionally, miR-21, miR-141, and miR-214 were found significantly deregulated in intermediate/high-risk PCa versus low-risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR-21, miR-375, and let-7c. CONCLUSIONS These findings suggest that the analysis of miRNAs--especially miRNA-21 and miR-375- in urine could be useful as biomarkers in PCa. Prostate 77: 573-583, 2017. © 2016 Wiley Periodicals, Inc. BACKGROUND MicroRNAs (miRNAs) are non‐coding small RNAs, involved in post‐transcriptional regulation of many target genes. METHODS Five miRNAs that have been consistently found deregulated in PCa (miR‐21, miR‐141, miR‐214, miR‐375, and let‐7c) were analyzed in urinary pellets from 60 prostate cancer (PCa) patients and 10 healthy subjects by qRT‐PCR. Besides, urinary exosomes were isolated by differential centrifugation and analyzed for those miRNAs. RESULTS Significant upregulation of miR‐21, miR‐141, and miR‐375 was found comparing PCa patients with healthy subjects in urinary pellets, while miR‐214 was found significantly downregulated. Regarding urinary exosomes, miR‐21 and miR‐375 were also significantly upregulated in PCa but no differences were found for miR‐141. Significant differences were found for let‐7c in PCa in urinary exosomes while no differences were observed in urinary pellets. A panel combining miR‐21 and miR‐375 is suggested as the best combination to distinguish PCa patients and healthy subjects, with an AUC of 0.872. Furthermore, the association of miRNAs with clinicopathological characteristics was investigated. MiR‐141 resulted significantly correlated with Gleason score in urinary pellets and let‐7c with clinical stage in urinary exosomes. Additionally, miR‐21, miR‐141, and miR‐214 were found significantly deregulated in intermediate/high‐risk PCa versus low‐risk/healthy subjects in urinary pellets. Significant differences between both groups were found in urinary exosomes for miR‐21, miR‐375, and let‐7c. CONCLUSIONS These findings suggest that the analysis of miRNAs—especially miRNA‐21 and miR‐375‐ in urine could be useful as biomarkers in PCa. Prostate 77: 573–583, 2017. © 2016 Wiley Periodicals, Inc. |
| Author | Serra, Marta Gavagnach, Montserrat Ferrer, Ferran Foj, Laura Giménez, Nuria Arévalo, Antonio Filella, Xavier |
| Author_xml | – sequence: 1 givenname: Laura surname: Foj fullname: Foj, Laura organization: Hospital Clínic, IDIBAPS – sequence: 2 givenname: Ferran surname: Ferrer fullname: Ferrer, Ferran organization: University of Barcelona, L'Hospitalet de Llobregat – sequence: 3 givenname: Marta surname: Serra fullname: Serra, Marta organization: Sant Cugat del Vallès – sequence: 4 givenname: Antonio surname: Arévalo fullname: Arévalo, Antonio organization: Sant Cugat del Vallès – sequence: 5 givenname: Montserrat surname: Gavagnach fullname: Gavagnach, Montserrat organization: Sant Cugat del Vallès – sequence: 6 givenname: Nuria surname: Giménez fullname: Giménez, Nuria organization: Fundació de Recerca Mútua Terrassa – sequence: 7 givenname: Xavier surname: Filella fullname: Filella, Xavier email: xfilella@clinic.cat organization: Hospital Clínic, IDIBAPS |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27990656$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkUtOwzAQhi0EoqWw4QAoEhs2KWM7juMlKuUhVbQqdG2lyQSlSuwSpwJ2HIEzchLcByxYsfLI883r_4_IvrEGCTml0KcA7HLZWNdnnCmxR7oUlAwBIrFPusAkhBHlskOOnFsAeBzYIekwqRTEIu6S2fDNOlunVZCaPHiw5uvj8_dr1pQmbd6Dupw-XLmgNMHEj2rTFoNBajJsgmtsMWtLazblPvtsrCvdMTko0srhye7tkdnN8GlwF47Gt_eDq1G44AmIkKJIUOUFF0wUuRJKylTliWQ0kjkrYioSRou5QMijTFGZAxeIMdIIlcrmCe-Ri21fL8HLCl2r69JlWFWpQbtymiYJlQIUk_9ABWWKi5h69PwPurCrxvhDPCUTHvs11tTZjlrNa8z1silrL5b-0dYDdAu8lhW-_-Yp6LVreu2a3rimJ9Px4ybi3_FCius |
| CODEN | PRSTDS |
| ContentType | Journal Article |
| Copyright | 2016 Wiley Periodicals, Inc. 2017 Wiley Periodicals, Inc. |
| Copyright_xml | – notice: 2016 Wiley Periodicals, Inc. – notice: 2017 Wiley Periodicals, Inc. |
| DBID | CGR CUY CVF ECM EIF NPM 7T5 7TO 8FD FR3 H94 K9. P64 RC3 7X8 |
| DOI | 10.1002/pros.23295 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Immunology Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Genetics Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) Immunology Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitleList | AIDS and Cancer Research Abstracts MEDLINE - Academic MEDLINE Genetics Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1097-0045 |
| EndPage | 583 |
| ExternalDocumentID | 4320941497 27990656 PROS23295 |
| Genre | article Journal Article |
| GroupedDBID | --- .3N .GA 05W 0R~ 10A 123 1L6 1OB 1OC 1ZS 33P 3SF 3WU 4.4 4ZD 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AAESR AAEVG AAHQN AAIPD AAMMB AAMNL AANLZ AAONW AASGY AAXRX AAYCA AAZKR ABCQN ABCUV ABIJN ABJNI ABLJU ABPVW ABQWH ABXGK ACAHQ ACCZN ACFBH ACGFS ACGOF ACMXC ACPOU ACPRK ACXBN ACXQS ADBBV ADBTR ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEFGJ AEGXH AEIGN AEIMD AENEX AEUYR AEYWJ AFBPY AFFPM AFGKR AFWVQ AFZJQ AGHNM AGXDD AGYGG AHBTC AHMBA AIACR AIDQK AIDYY AITYG AIURR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN ALVPJ AMBMR AMYDB ATUGU AZBYB AZVAB BAFTC BDRZF BFHJK BHBCM BMXJE BROTX BRXPI BY8 C45 CS3 D-6 D-7 D-E D-F DCZOG DPXWK DR2 DRFUL DRMAN DRSTM DU5 EBD EBS EJD EMOBN F00 F01 F04 F5P FUBAC G-S G.N GNP GODZA H.X HBH HGLYW HHY HHZ HZ~ IX1 J0M JPC KBYEO KQQ LATKE LAW LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM N04 N05 N9A NF~ NNB O66 O9- OIG OVD P2P P2W P2X P2Z P4B P4D PQQKQ Q.N Q11 QB0 QRW R.K ROL RX1 RYL SUPJJ SV3 TEORI UB1 V2E W8V W99 WBKPD WHWMO WIB WIH WIJ WIK WJL WOHZO WQJ WVDHM WXI WXSBR XG1 XV2 ZZTAW ~IA ~WT .GJ .Y3 31~ 3O- 53G AAHHS AANHP ABEML ACBWZ ACCFJ ACRPL ACSCC ACYXJ ADNMO AEEZP AEQDE AEUQT AFFNX AFPWT AIWBW AJBDE ASPBG AVWKF AZFZN CGR CUY CVF ECM EIF FEDTE HF~ HVGLF M6P NPM PALCI RIWAO RJQFR RWI SAMSI WRC WUP WWO ZGI ZXP 7T5 7TO 8FD FR3 H94 K9. O8X P64 RC3 7X8 |
| ID | FETCH-LOGICAL-j3805-1e58e9df3525fd95977a9d872147d2f615821fb5e0d4c917d035ee6e14e99cb83 |
| IEDL.DBID | DRFUL |
| ISICitedReferencesCount | 145 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000397496300002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0270-4137 1097-0045 |
| IngestDate | Thu Oct 02 05:43:22 EDT 2025 Fri Jul 11 09:40:36 EDT 2025 Sat Nov 29 14:31:15 EST 2025 Wed Feb 19 02:00:14 EST 2025 Sun Sep 21 06:18:26 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | miRNA prostate cancer urine exosomes |
| Language | English |
| License | 2016 Wiley Periodicals, Inc. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-j3805-1e58e9df3525fd95977a9d872147d2f615821fb5e0d4c917d035ee6e14e99cb83 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| PMID | 27990656 |
| PQID | 1878360351 |
| PQPubID | 1016443 |
| PageCount | 11 |
| ParticipantIDs | proquest_miscellaneous_1881750927 proquest_miscellaneous_1851293561 proquest_journals_1878360351 pubmed_primary_27990656 wiley_primary_10_1002_pros_23295_PROS23295 |
| PublicationCentury | 2000 |
| PublicationDate | May 1, 2017 |
| PublicationDateYYYYMMDD | 2017-05-01 |
| PublicationDate_xml | – month: 05 year: 2017 text: May 1, 2017 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Hoboken |
| PublicationTitle | The Prostate |
| PublicationTitleAlternate | Prostate |
| PublicationYear | 2017 |
| Publisher | Wiley Subscription Services, Inc |
| Publisher_xml | – name: Wiley Subscription Services, Inc |
| References | 2004; 101 2015; 1 2009; 69 2011; 157 2015; 6 2010; 107 2015; 72 2015; 166 2015; 53 2016; 76 2016; 52 2011; 32 2008; 105 2016; 70 2008; 3 2015; 1218 2013; 7 2013; 8 2016; 17 2011; 6 2012; 106 2012; 30 2009; 28 2016; 11 2012; 72 2015; 67 2016; 6 2012; 131 2015; 25 2000; 18 2013; 32 2015; 61 2011; 71 2015; 87 2016; 20 2014; 14 2014; 13 2014; 19 2009; 383 2008; 179 2014; 9 2012; 7 2007; 67 2016; 69 2016; 9 2014; 33 2006; 103 2016; 172 |
| References_xml | – volume: 103 start-page: 2257 year: 2006 end-page: 2261 article-title: A microRNA expression signature of human solid tumors defines cancer gene targets publication-title: Proc Natl Acad Sci USA – volume: 8 year: 2013 end-page: e69239 article-title: Circulating microRNA profiling identifies a subset of metastatic prostate cancer patients with evidence of cancer associated hypoxia publication-title: PLoS ONE – volume: 11 start-page: e0157566 year: 2016 article-title: Comparative study of extracellular vesicles from the urine of healthy individuals and prostate cancer patients publication-title: PLoS ONE – volume: 25 start-page: 238 year: 2015 end-page: 245 article-title: Should Gleason 6 be labeled as cancer publication-title: Curr Opin Urol – volume: 33 start-page: 4097 year: 2014 end-page: 4106 article-title: Androgen receptor and microRNA‐21 axis downregulates transforming growth factor beta receptor II (TGFBR2) expression in prostate cancer publication-title: Oncogene – volume: 53 start-page: 1109 year: 2015 end-page: 1118 article-title: Urinary miR‐183 and miR‐205 do not surpass PCA3 in urine as predictive markers for prostate biopsy outcome despite their highly dysregulated expression in prostate cancer tissue publication-title: Clin Chem Lab Med – volume: 76 start-page: 986 year: 2016 end-page: 993 article-title: Association of microRNA‐21 expression with clinicopathological characteristics and the risk of progression in advanced prostate cancer patients receiving androgen deprivation therapy publication-title: Prostate – volume: 13 start-page: 227 year: 2014 end-page: 239 article-title: MiR‐888 is an expressed prostatic secretions‐derived microRNA that promotes prostate cell growth and migration publication-title: Cell Cycle – volume: 8 start-page: e76994 year: 2013 article-title: MicroRNA profiling in prostate cancer—The diagnostic potential of urinary miR‐205 and miR‐214 publication-title: PLoS ONE – volume: 9 start-page: e91729 year: 2014 article-title: Curated microRNAs in urine and blood fail to validate as predictive biomarkers for high‐risk prostate cancer publication-title: PLoS ONE – volume: 87 start-page: 26 year: 2015 end-page: 30 article-title: An optimized procedure for exosome isolation and analysis using serum samples: Application to cancer biomarker discovery publication-title: Methods – volume: 166 start-page: 474 year: 2015 end-page: 484 article-title: Kidney tissue proteomics reveals regucalcin downregulation in response to diabetic nephropathy with reflection in urinary exosomes publication-title: Transl Res – volume: 18 start-page: 1164 year: 2000 end-page: 1172 article-title: Clinical utility of the percentage of positive prostate biopsies in defining biochemical outcome after radical prostatectomy for patients with clinically localized prostate cancer publication-title: J Clin Oncol – volume: 1218 start-page: 439 year: 2015 end-page: 463 article-title: Urinary MicroRNAs as a new class of noninvasive biomarkers in oncology, nephrology, and cardiology publication-title: Methods Mol Biol – volume: 71 start-page: 213 year: 2011 end-page: 217 article-title: MicroRNA determination in urine for prostate cancer detection in Mexican patients at the Hospital General “Dr. Manuel Gea González publication-title: Rev Mex Urol – volume: 19 start-page: 1568 year: 2014 end-page: 1575 article-title: MiRNA in plasma exosome is stable under different storage conditions publication-title: Molecules – volume: 69 start-page: 557 year: 2016 end-page: 560 article-title: Genomic correlates to the newly proposed grading prognostic groups for prostate cancer publication-title: Eur Urol – volume: 69 start-page: 7165 year: 2009 end-page: 7169 article-title: MiR‐21: An androgen receptor‐regulated microRNA that promotes hormone‐dependent and hormone‐independent prostate cancer growth publication-title: Cancer Res – volume: 7 start-page: 769 year: 2013 end-page: 778 article-title: Exosomes: The future of biomarkers in medicine publication-title: Biomark Med – volume: 7 start-page: e32832 year: 2012 article-title: MicroRNA let‐7c is downregulated in prostate cancer and suppresses prostate cancer growth publication-title: PLoS ONE – volume: 72 start-page: 3531 year: 2015 end-page: 3542 article-title: MicroRNA: Master controllers of intracellular signaling pathways publication-title: Cell Mol Life Sci – volume: 9 year: 2016 article-title: MiRNAs as novel biomarkers in the management of prostate cancer publication-title: Clin Chem Lab Med – volume: 105 start-page: 10513 year: 2008 end-page: 10518 article-title: Circulating microRNAs as stable blood‐based markers for cancer detection publication-title: Proc Natl Acad Sci – volume: 9 start-page: e98597 year: 2014 article-title: MiR‐19, miR‐345, miR‐519c‐5p serum levels predict adverse pathology in prostate cancer patients eligible for active surveillance publication-title: PLoS ONE – volume: 70 start-page: 312 year: 2016 end-page: 322 article-title: The potential of MicroRNAs as prostate cancer biomarkers publication-title: Eur Urol – volume: 14 start-page: 82 year: 2014 article-title: Elevated expression of prostate cancer‐associated genes is linked to down‐regulation of microRNAs publication-title: BMC Cancer – volume: 28 start-page: 369 year: 2009 end-page: 378 article-title: MicroRNA function in cancer: Oncogene or a tumor suppressor publication-title: Cancer Metastasis Rev – volume: 3 start-page: 1101 year: 2008 end-page: 1108 article-title: Analyzing real‐time PCR data by the comparative C(T) method publication-title: Nat Protoc – volume: 30 start-page: 4294 year: 2012 end-page: 4296 article-title: Gleason score 6 adenocarcinoma: Should it be labeled as cancer publication-title: J Clin Oncol – volume: 157 start-page: 157 year: 2011 end-page: 162 article-title: MicroRNAs: miRRORS of health and disease publication-title: Transl Res – volume: 172 start-page: 61 year: 2016 end-page: 72 article-title: MicroRNA‐141 is upregulated in preeclamptic placentae and regulates trophoblast invasion and intercellular communication publication-title: Transl Res – volume: 32 start-page: 583 year: 2011 end-page: 588 article-title: Investigation of miR‐21, miR‐141, and miR‐221 in blood circulation of patients with prostate cancer publication-title: Tumour Biol – volume: 106 start-page: 768 year: 2012 end-page: 774 article-title: Changes in circulating microRNA levels associated with prostate cancer publication-title: Br J Cancer – volume: 61 start-page: 56 year: 2015 end-page: 63 article-title: Circulating microRNA biomarker studies: Pitfalls and potential solutions publication-title: Clin Chem – volume: 52 start-page: 116 year: 2016 end-page: 124 article-title: Detection of miRNAs in urine of prostate cancer patients publication-title: Medicina (Kaunas) – volume: 20 year: 2016 article-title: Ago‐RIP‐Seq identifies Polycomb repressive complex I member CBX7 as a major target of miR‐375 in prostate cancer progression publication-title: Oncotarget – volume: 6 start-page: e19139 year: 2011 article-title: MiR‐21 induced angiogenesis through AKT and ERK activation and HIF‐1a expression publication-title: PLoS ONE – volume: 6 start-page: 292 year: 2015 end-page: 301 article-title: Controlling reck miR21 promotes tumor cell invasion and is related to biochemical recurrence in prostate cancer publication-title: J Cancer – volume: 107 start-page: 2610 year: 2010 end-page: 2615 article-title: Basal epitelial stem cells are efficient targets for prostate cancer initiation publication-title: Proc Natl Acad Sci USA – volume: 67 start-page: 6130 year: 2007 end-page: 6135 article-title: MicroRNA expression profiling in prostate cancer publication-title: Cancer Res – volume: 1 start-page: E359 issue: 136 year: 2015 end-page: E386 article-title: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN publication-title: Int J Cancer – volume: 131 start-page: 652 year: 2012 end-page: 661 article-title: Discovery of circulating microRNAs associated with human prostate cancer using a mouse model of disease publication-title: Int J Cancer – volume: 32 start-page: 1843 year: 2013 end-page: 1853 article-title: BTG2 loss and miR‐21 upregulation contribute to prostate cell transformation by inducing luminal markers expression and epithelial‐mesenchymal transition publication-title: Oncogene – volume: 72 start-page: 1443 year: 2012 end-page: 1452 article-title: A panel of five circulating microRNAs as potential biomarkers for prostate cancer publication-title: Prostate – volume: 76 start-page: 68 year: 2016 end-page: 79 article-title: Lectin‐induced agglutination method of urinary exosomes isolation followed by mi‐RNA analysis: Application for prostate cancer diagnostic publication-title: Prostate – volume: 17 year: 2016 article-title: MicroRNA‐21 regulates the ERK/NF‐κB signaling pathway to affect the proliferation, migration and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4 and PTEN publication-title: Mol Carcinog – volume: 179 start-page: 1354 year: 2008 end-page: 1360 article-title: Mayo Clinic validation of the D'amico risk group classification for predicting survival following radical prostatectomy publication-title: J Urol – volume: 67 start-page: 33 year: 2015 end-page: 41 article-title: Exosomal miR‐1290 and miR‐375 as prognostic markers in castration‐resistant prostate cancer publication-title: Eur Urol – volume: 383 start-page: 280 year: 2009 end-page: 285 article-title: MicroRNA‐21 directly targets MARCKS and promotes apoptosis resistance and invasion in prostate cancer cells publication-title: Biochem Biophys Res Commun – volume: 6 year: 2016 article-title: A ZEB1‐miR‐375‐YAP1 pathway regulates epithelial plasticity in prostate cancer publication-title: Oncogene – volume: 101 start-page: 13368 year: 2004 end-page: 13373 article-title: Identification and proteomic profiling of exosomes in human urine publication-title: Proc Natl Acad Sci USA |
| SSID | ssj0010002 |
| Score | 2.5517533 |
| Snippet | BACKGROUND
MicroRNAs (miRNAs) are non‐coding small RNAs, involved in post‐transcriptional regulation of many target genes.
METHODS
Five miRNAs that have been... MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. Five miRNAs that have been consistently found... BACKGROUND MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes. METHODS Five miRNAs that have been... MicroRNAs (miRNAs) are non-coding small RNAs, involved in post-transcriptional regulation of many target genes.BACKGROUNDMicroRNAs (miRNAs) are non-coding... |
| SourceID | proquest pubmed wiley |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 573 |
| SubjectTerms | Aged Aged, 80 and over Biomarkers, Tumor - genetics Biomarkers, Tumor - urine exosomes Exosomes - genetics Exosomes - metabolism Humans Male MicroRNAs - genetics MicroRNAs - urine Middle Aged miRNA Prognosis prostate cancer Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - urine urine |
| Title | Exosomal and Non‐Exosomal Urinary miRNAs in Prostate Cancer Detection and Prognosis |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fpros.23295 https://www.ncbi.nlm.nih.gov/pubmed/27990656 https://www.proquest.com/docview/1878360351 https://www.proquest.com/docview/1851293561 https://www.proquest.com/docview/1881750927 |
| Volume | 77 |
| WOSCitedRecordID | wos000397496300002&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVWIB databaseName: Wiley Online Library Full Collection 2020 customDbUrl: eissn: 1097-0045 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0010002 issn: 0270-4137 databaseCode: DRFUL dateStart: 19960101 isFulltext: true titleUrlDefault: https://onlinelibrary.wiley.com providerName: Wiley-Blackwell |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bS8MwFD5sU8QX75fplAo-CdU1vSQBX8bc8EHnmA72VtrmBCbYyjrFR3-Cv9FfYpLWiiCC-FaSEyg5l3y5nO8AHHOpLNd1pc2FELbH4sSOpFQbV8k9TCTGkVsUm6CDAZtM-LAG55-5MAU_RHXgpj3DxGvt4FGcn32RhqoAk58qPMD9OiwQZbh-AxYuRv3xVXWLoN3dnLHQtq2CNa3oScnZ1-ifoOV3pGqWmv7q_35yDVZKiGl1CptYhxqmG7B0XV6ib8K495Ll2YMSiVJhDbL0_fWtahrPTIKu9TAdDTq5NU2toc4LUYjU6moDmVkXODfPt1IzXPXqp3rTfAvG_d5d99IuqyvY9y7T9KPoM-RCaj5UKbjmoYu4YFQXLhJEKqTDiCNjH9vCS9SmTqgZRgzQ8ZDzJGbuNjTSLMVdsAIqA46x52FEvBhdrlOlEypZINwg8HkTWp9THJYukocOMwkkru804ajqVsatbyyiFLMnLWPwiMJ4v8kwR8MeQpuwU6gvfCyYOkJC1WKrEGsTToyWqo6CrJmEWj-h0U84HN3cmq-9vwjvwzLRC715AtmCxnz2hAewmDzPp_nsEOp0wg5Ls_wAN2TmAg |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1ba9swFD506Vj3snZbL9m61YM9DdzEli_SY8mFjCVeyBLIm7GtI0ghdomTssf9hP3G_ZLpyJ7DYBRG34x1BMbnO9In6ZxPAB-F0shlTNlCSml7PM3sRCm9cFXCw0xhmrDqsokwivhyKaZ1bg7VwlT6EM2GG0WGGa8pwGlDurNXDdUjTHmtCYHwn8Chp3GkAX7Ynw0X4-YYgeLdbLKEXVuP1mGjT-p29r3_xS3_pqpmrhkeP_IrT-BFTTKtmwoVL-EA81fwbFIfo7-GxeB7URZrbZLk0oqK_NePn82rxcaU6Frr1Sy6Ka1Vbk2pMkRzUqtHENlYfdyaBK7cdNetlKy3Kk9hMRzMeyO7vl_BvmWcBEjR5yikIkVUJQUp0SVC8pCuLpKu0lyHu45KfexKL9PLOtllPmKAjodCZClnZ9DKixwvwApCFQhMPQ8T10uRCSqWzkLFA8mCwBdtuPzzj-M6SMrY4aaEhPlOGz40zRredGaR5FjsyMYwEs3yHrLhDhEfN2zDeeW_-K7S6ojdUE-3mrO24ZNxU9NQyTW7MfknNv6Jp7Ov38zTm_8xvoKj0Xwyjsefoy9v4blL075JiLyE1nazw3fwNLvfrsrN-xqdvwGTbOkK |
| linkToPdf | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bS-wwEB50FfHF-2W9nQrnSahum16SR3FdlKN1Wc-Cb6VtJrCCrWxX8dGf4G_0l5hJa0UQQXwrzQRKZyb5kpn5BuCvUNpyGVO2kFLaHk8zO1FKH1yV8DBTmCasajYRRhG_uRH9OjeHamEqfojmwo08w6zX5OB4L9XRB2uoXmHKQw0IhD8NMx51kWnBTHfQG140YQTyd3PJEnZsvVqHDT-pe_Qx-yts-Rmqmr2mt_jLr1yChRpkWseVVSzDFOYrMHdZh9FXYXj6VJTFnRZJcmlFRf76_NK8Go5Nia51NxpEx6U1yq0-VYZoTGqdkImMrS5OTAJXbqbrUUrWG5VrMOyd_j85s-v-CvYt40RAij5HIRUxoiopiIkuEZKH1LpIukpjHe46KvWxI71MH-tkh_mIAToeCpGlnK1DKy9y3AQrCFUgMPU8TFwvRSaoWDoLFQ8kCwJftGHn_R_HtZOUscNNCQnznTbsN8PavClmkeRYPJCMQSQa5X0nwx0CPm7Yho1Kf_F9xdURu6HebjVmbcOBUVMzUNE1uzHpJzb6ifuDq2vztPUT4T8w1-_24ovz6N82zLu065t8yB1oTcYPuAuz2eNkVI73auN8A0C56IU |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Exosomal+and+Non-Exosomal+Urinary+miRNAs+in+Prostate+Cancer+Detection+and+Prognosis&rft.jtitle=The+Prostate&rft.au=Foj%2C+Laura&rft.au=Ferrer%2C+Ferran&rft.au=Serra%2C+Marta&rft.au=Ar%C3%A9valo%2C+Antonio&rft.date=2017-05-01&rft.issn=1097-0045&rft.eissn=1097-0045&rft.volume=77&rft.issue=6&rft.spage=573&rft_id=info:doi/10.1002%2Fpros.23295&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0270-4137&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0270-4137&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0270-4137&client=summon |