Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses
Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses" indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate fu...
Uložené v:
| Vydané v: | Proceedings of the National Academy of Sciences - PNAS Ročník 114; číslo 26; s. E5246 |
|---|---|
| Hlavní autori: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
27.06.2017
|
| ISSN: | 1091-6490, 1091-6490 |
| On-line prístup: | Zistit podrobnosti o prístupe |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses" indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1-10) and 2.03 at 4 wk (range: 1-4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1-8) and 2.37 at 4 wk (range: 1-5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels.Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses" indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1-10) and 2.03 at 4 wk (range: 1-4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1-8) and 2.37 at 4 wk (range: 1-5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels. |
|---|---|
| AbstractList | Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses" indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1-10) and 2.03 at 4 wk (range: 1-4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1-8) and 2.37 at 4 wk (range: 1-5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels.Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses" indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1-10) and 2.03 at 4 wk (range: 1-4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1-8) and 2.37 at 4 wk (range: 1-5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels. |
| Author | Kaufmann, Walter A Malagon, Gerardo Miki, Takafumi Marty, Alain Watanabe, Masahiko Gomez, Laura Tabuchi, Katsuhiko Shigemoto, Ryuichi |
| Author_xml | – sequence: 1 givenname: Takafumi surname: Miki fullname: Miki, Takafumi – sequence: 2 givenname: Walter A surname: Kaufmann fullname: Kaufmann, Walter A – sequence: 3 givenname: Gerardo surname: Malagon fullname: Malagon, Gerardo – sequence: 4 givenname: Laura surname: Gomez fullname: Gomez, Laura – sequence: 5 givenname: Katsuhiko surname: Tabuchi fullname: Tabuchi, Katsuhiko – sequence: 6 givenname: Masahiko surname: Watanabe fullname: Watanabe, Masahiko – sequence: 7 givenname: Ryuichi surname: Shigemoto fullname: Shigemoto, Ryuichi – sequence: 8 givenname: Alain surname: Marty fullname: Marty, Alain |
| BookMark | eNpNjTFPwzAUhC1UJNrCzOoRCaXYTurEI6qgIFWwwFy9OM-ti-uEPAepM38cCgxM9w333U3YKLYRGbuUYiZFmd90EWgmS1EUpZCyOGFjKYzMdGHE6B-fsQnRTghh5pUYs8-nYV9jT7x1vOuRDhG65C1fgLrmdgsxYuA2DJSOpT0ku-Vp2xIeBTdEm3wbIYQDb9D5iA3_QPJ2CNDzprVvPm44-YTEffyGuAnILcbUQ-A_Z4R0zk4dBMKLv5yy1_u7l8VDtnpePi5uV9ku12XKDM4rW1sNGpVVFTorc1BaoMN5DaJQGrGsZV4V1lQlgHY617oEI8HoJtdqyq5-d7u-fR-Q0nrvyWIIELEdaC2NMCo3RWXUFxYeaXg |
| ContentType | Journal Article |
| DBID | 7X8 |
| DOI | 10.1073/pnas.1704470114 |
| DatabaseName | MEDLINE - Academic |
| DatabaseTitle | MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Sciences (General) |
| EISSN | 1091-6490 |
| GroupedDBID | --- -DZ -~X .55 0R~ 123 29P 2AX 2FS 2WC 4.4 53G 5RE 5VS 7X8 85S AACGO AAFWJ AANCE ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACHIC ACIWK ACNCT ACPRK ADQXQ ADULT AENEX AEUPB AEXZC AFFNX AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM BKOMP CS3 D0L DCCCD DIK DU5 E3Z EBS EJD F5P FRP GX1 H13 HH5 HYE IPSME JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JST KQ8 L7B LU7 N9A N~3 O9- OK1 PNE PQQKQ R.V RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR W8F WH7 WOQ WOW X7M XSW Y6R YBH YKV YSK ZCA ~02 ~KM |
| ID | FETCH-LOGICAL-j367t-9e58cbc6a6e2c28efc13a260efe5ba0426ee7b1384c987aa6f63667a91a96d362 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 63 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000404108400028&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1091-6490 |
| IngestDate | Fri Sep 05 10:18:36 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 26 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-j367t-9e58cbc6a6e2c28efc13a260efe5ba0426ee7b1384c987aa6f63667a91a96d362 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://www.pnas.org/doi/10.1073/pnas.1704470114 |
| PQID | 1909239489 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_1909239489 |
| PublicationCentury | 2000 |
| PublicationDate | 2017-06-27 |
| PublicationDateYYYYMMDD | 2017-06-27 |
| PublicationDate_xml | – month: 06 year: 2017 text: 2017-06-27 day: 27 |
| PublicationDecade | 2010 |
| PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
| PublicationYear | 2017 |
| SSID | ssj0009580 |
| Score | 2.4857097 |
| Snippet | Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such "simple synapses"... |
| SourceID | proquest |
| SourceType | Aggregation Database |
| StartPage | E5246 |
| Title | Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses |
| URI | https://www.proquest.com/docview/1909239489 |
| Volume | 114 |
| WOSCitedRecordID | wos000404108400028&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3PS91AEF60eujFVm2xassIHhTZmmTj_jiJiNLTw4PCu8nsZhZfeSTafQqe_cfdycujQi8F7wkZlpn5vpmdzCfEvlfeaF9EGSgaWWcEkIgmSBfQNIEyQ4hNLzZhRiM7HruroeGWhrHKRU7sE3XTBe6RH2fgcizjbd3p_YNk1Si-XR0kNJbFispUhgPTjO2bpbt2vo3AlVLXrlis9jHq-L7F9LM0RV0bLgn-ycQ9vFx-eq9hn8XaQCzhbO4J62KJ2g2xPoRugoNhv_ThpngZ9SogCboIPAb73GLOGwHOsToC_hG4pSmE6SOvUEiQGW24g9ldl4hfYByctw-nz9BQzCS1gSdKk36eFbK53HsHNi7BpAVuRUwJhhlQ6D-WKH0RN5cX1-e_5KDFIH8rbWbS0YkNPmjUVIXKUgylwlwLUaQTj1yIERlfKlsHZw2ijlppbdCV6HSTUfKr-NB2LW0JKMrsG0qZGrWrNXqvXKZRGRfJo8XCfRN7i4O-zb7OFxjYUveYbv8e9fZ_PLMjPlaMwYWWldkVKzHHM30Xq-FpNkl_fvSu8golncxp |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Numbers+of+presynaptic+Ca2%2B+channel+clusters+match+those+of+functionally+defined+vesicular+docking+sites+in+single+central+synapses&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Miki%2C+Takafumi&rft.au=Kaufmann%2C+Walter+A&rft.au=Malagon%2C+Gerardo&rft.au=Gomez%2C+Laura&rft.date=2017-06-27&rft.issn=1091-6490&rft.eissn=1091-6490&rft.volume=114&rft.issue=26&rft.spage=E5246&rft_id=info:doi/10.1073%2Fpnas.1704470114&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1091-6490&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1091-6490&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1091-6490&client=summon |