Polymorphism and estimation of the number of MhcCyca class I and class II genes in laboratory strains of the common carp (Cyprinus carpio L.)

Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific probes. The K1-5 and K2-1 probes were obtained as PCR products after amplification of genomic DNA from a European carp using primers deduced...

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Published in:Developmental and comparative immunology Vol. 17; no. 2; p. 141
Main Authors: Stet, R J, van Erp, S H, Hermsen, T, Sültmann, H A, Egberts, E
Format: Journal Article
Language:English
Published: United States 1993
Subjects:
Amino Acid Sequence
Animals
Animals, Laboratory - genetics
Animals, Laboratory - immunology
Base Sequence
Breeding
Carps - genetics
Carps - immunology
Female
Genes, MHC Class I
Genes, MHC Class II
Male
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Sequence Alignment
Sequence Homology
Serology
ISSN:0145-305X
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Abstract Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific probes. The K1-5 and K2-1 probes were obtained as PCR products after amplification of genomic DNA from a European carp using primers deduced from genomic sequences, and were shown to be 90% and 80% similar to Cyca-Z exon 3 and Cyca-YB exon 2 sequences, respectively. Six carp strains of different geographical origins and genomic status were studied. In homozygous gynogenetic carp strains the class I probe K1-5 hybridized to 9-12 fragments, whereas the class II probe K2-1 hybridized to 3-5 fragments. Thus, the Cyca consists of multiple class I and class II genes. The level of polymorphism of the Cyca genes of the strains studied was calculated as the percentage of polymorphic fragments among the total number of fragments observed, and was shown to be 70% for class I and 40-66% for class II genes. In addition, a possible correlation was investigated between a serologically defined locus K, which was demonstrated previously to incorporate class I-like characteristics, and molecular genotyping using the class I probe. Two gynogenetic families, which were serologically typed K1 and K2 homozygous, also differed in their RFLPs using a class I probe. This would suggest that the K locus is part of the Cyca complex.
AbstractList Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific probes. The K1-5 and K2-1 probes were obtained as PCR products after amplification of genomic DNA from a European carp using primers deduced from genomic sequences, and were shown to be 90% and 80% similar to Cyca-Z exon 3 and Cyca-YB exon 2 sequences, respectively. Six carp strains of different geographical origins and genomic status were studied. In homozygous gynogenetic carp strains the class I probe K1-5 hybridized to 9-12 fragments, whereas the class II probe K2-1 hybridized to 3-5 fragments. Thus, the Cyca consists of multiple class I and class II genes. The level of polymorphism of the Cyca genes of the strains studied was calculated as the percentage of polymorphic fragments among the total number of fragments observed, and was shown to be 70% for class I and 40-66% for class II genes. In addition, a possible correlation was investigated between a serologically defined locus K, which was demonstrated previously to incorporate class I-like characteristics, and molecular genotyping using the class I probe. Two gynogenetic families, which were serologically typed K1 and K2 homozygous, also differed in their RFLPs using a class I probe. This would suggest that the K locus is part of the Cyca complex.Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific probes. The K1-5 and K2-1 probes were obtained as PCR products after amplification of genomic DNA from a European carp using primers deduced from genomic sequences, and were shown to be 90% and 80% similar to Cyca-Z exon 3 and Cyca-YB exon 2 sequences, respectively. Six carp strains of different geographical origins and genomic status were studied. In homozygous gynogenetic carp strains the class I probe K1-5 hybridized to 9-12 fragments, whereas the class II probe K2-1 hybridized to 3-5 fragments. Thus, the Cyca consists of multiple class I and class II genes. The level of polymorphism of the Cyca genes of the strains studied was calculated as the percentage of polymorphic fragments among the total number of fragments observed, and was shown to be 70% for class I and 40-66% for class II genes. In addition, a possible correlation was investigated between a serologically defined locus K, which was demonstrated previously to incorporate class I-like characteristics, and molecular genotyping using the class I probe. Two gynogenetic families, which were serologically typed K1 and K2 homozygous, also differed in their RFLPs using a class I probe. This would suggest that the K locus is part of the Cyca complex.
Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific probes. The K1-5 and K2-1 probes were obtained as PCR products after amplification of genomic DNA from a European carp using primers deduced from genomic sequences, and were shown to be 90% and 80% similar to Cyca-Z exon 3 and Cyca-YB exon 2 sequences, respectively. Six carp strains of different geographical origins and genomic status were studied. In homozygous gynogenetic carp strains the class I probe K1-5 hybridized to 9-12 fragments, whereas the class II probe K2-1 hybridized to 3-5 fragments. Thus, the Cyca consists of multiple class I and class II genes. The level of polymorphism of the Cyca genes of the strains studied was calculated as the percentage of polymorphic fragments among the total number of fragments observed, and was shown to be 70% for class I and 40-66% for class II genes. In addition, a possible correlation was investigated between a serologically defined locus K, which was demonstrated previously to incorporate class I-like characteristics, and molecular genotyping using the class I probe. Two gynogenetic families, which were serologically typed K1 and K2 homozygous, also differed in their RFLPs using a class I probe. This would suggest that the K locus is part of the Cyca complex.
Author Sültmann, H A
Hermsen, T
van Erp, S H
Egberts, E
Stet, R J
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Snippet Restriction fragment length polymorphisms (RFLPs) have been identified in the Mhc of the carp (MhcCyca) using class I (Cyca-Z) and class II (Cyca-YB) specific...
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StartPage 141
SubjectTerms Amino Acid Sequence
Animals
Animals, Laboratory - genetics
Animals, Laboratory - immunology
Base Sequence
Breeding
Carps - genetics
Carps - immunology
Female
Genes, MHC Class I
Genes, MHC Class II
Male
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Sequence Alignment
Sequence Homology
Serology
Title Polymorphism and estimation of the number of MhcCyca class I and class II genes in laboratory strains of the common carp (Cyprinus carpio L.)
URI https://www.ncbi.nlm.nih.gov/pubmed/8099021
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