Mechanism of the AppABLUF Photocycle Probed by Site-Specific Incorporation of Fluorotyrosine Residues: Effect of the Y21 pKa on the Forward and Reverse Ground-State Reactions

The transcriptional antirepressor AppA is a blue light using flavin (BLUF) photoreceptor that releases the transcriptional repressor PpsR upon photoexcitation. Light activation of AppA involves changes in a hydrogen-bonding network that surrounds the flavin chromophore on the nanosecond time scale,...

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Veröffentlicht in:	Journal of the American Chemical Society Jg. 138; H. 3; S. 926
Hauptverfasser:	Gil, Agnieszka, Haigney, Allison, Laptenok, Sergey P, Brust, Richard, Lukacs, Andras, Iuliano, James, Jeng, Jessica, Melief, Eduard, Zhao, Rui-Kun, Yoon, EunBin, Clark, Ian, Towrie, Michael, Greetham, Gregory M, Ng, Annabelle, Truglio, James, French, Jarrod, Meech, Stephen R, Tonge, Peter J
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 27.01.2016
Schlagworte:	Bacterial Proteins - chemistry Flavoproteins - chemistry Fluorine - chemistry Hydrogen Bonding Hydrogen-Ion Concentration Models, Molecular Molecular Structure Photochemical Processes Quantum Theory Tyrosine - analogs & derivatives Tyrosine - chemistry
ISSN:	1520-5126, 1520-5126
Online-Zugang:	Weitere Angaben
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