Peripheral Blood-Derived Endothelial Progenitor Cells Enhance Vertical Bone Formation

Background This study presents a novel cell‐based approach for extra‐cortical bone regeneration. Objective To enhance vertical bone formation by combining guided bone regeneration and transplantation of peripheral blood‐derived endothelial progenitor cells (EPCs) in a rat calvaria model. Materials a...

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Published in:Clinical implant dentistry and related research Vol. 17; no. 1; pp. 83 - 92
Main Authors: Zigdon-Giladi, Hadar, Bick, Tova, Morgan, Elise F., Lewinson, Dina, Machtei, Eli E.
Format: Journal Article
Language:English
Published: United States Blackwell Publishing Ltd 01.02.2015
Subjects:
Animals
bone regeneration
Bone Regeneration - physiology
Calcium Phosphates - pharmacology
Cell Culture Techniques
cell therapy
Collagen - pharmacology
Drug Combinations
Endothelium, Vascular - cytology
Fibronectins - pharmacology
Flow Cytometry
Laminin - pharmacology
Male
microcomputed tomography
peripheral blood-derived endothelial progenitor cells
Proteoglycans - pharmacology
Rats
Rats, Inbred Lew
Skull - surgery
Stem Cell Transplantation
Stem Cells - physiology
tissue engineering
X-Ray Microtomography
bone regeneration
tissue engineering
microcomputed tomography
peripheral blood-derived endothelial progenitor cells
cell therapy
ISSN:1523-0899, 1708-8208, 1708-8208
Online Access:Get full text
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Summary:Background This study presents a novel cell‐based approach for extra‐cortical bone regeneration. Objective To enhance vertical bone formation by combining guided bone regeneration and transplantation of peripheral blood‐derived endothelial progenitor cells (EPCs) in a rat calvaria model. Materials and Methods EPCs were isolated from peripheral blood of inbred rats. Gold domes (7 mm radius, 5 mm height) were filled with β‐tricalcium phosphate (βTCP) mixed with 5 × 105 EPC. Domes filled with βTCP served as control (CNT). Rats were sacrificed after 3 months. Vertical bone augmentation was analyzed using histology, histomorphometry, and microcomputed tomography (μCT). Results In all rats, hard tissue filled the space under the dome. Histomorphometric analysis revealed that EPC transplantations doubled vertical bone height (EPC 4.04 ± 0.22 mm vs CNT 2.29 ± 0.22 mm, p ≤ .001). EPC also caused ∼50% increase in bone area fraction (EPC 47.3 ± 3.1% vs CNT 31.1 ± 2.7%, p ≤ .003). μCT results also showed that bone volume fraction (BV/TV) was higher in EPC group (p = .0169). In both groups, BV/TV declined from the bottom to the top of the samples. No differences in tissue mineral density were found between EPC and CNT groups. Conclusion EPC transplantation significantly improved bone formation especially in the areas that are remote from the original bone.
Bibliography:ArticleID:CID12078
Rambam Health Care Campus, Haifa, Israel
ark:/67375/WNG-N9WKF8X1-4
istex:B8456B35BE2FEA2B827C1098575733D315DFE38F
Ministry of Industry Trade and Labor, Israel Government - No. 46293
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1523-0899
1708-8208
1708-8208
DOI:10.1111/cid.12078