Co-Transplantation of Endothelial Progenitor Cells and Mesenchymal Stem Cells Promote Neovascularization and Bone Regeneration

Background Bone formation relies on sufficient blood supply and osteoprogenitor cells. Purpose The study aims to evaluate the influence of endothelial progenitor cells (EPCs) in combination with mesenchymal stem cells (MSCs) on early vascularization and intramembranous bone regeneration. Materials a...

Full description

Saved in:
Bibliographic Details
Published in:Clinical implant dentistry and related research Vol. 17; no. 2; pp. 353 - 359
Main Authors: Zigdon-Giladi, Hadar, Bick, Tova, Lewinson, Dina, Machtei, Eli E.
Format: Journal Article
Language:English
Published: United States Blackwell Publishing Ltd 01.04.2015
Subjects:
Animals
bone regeneration
bone tissue engineering
Calcium Phosphates
cell therapy
endothelial progenitor cells
Endothelial Progenitor Cells - transplantation
Mesenchymal Stem Cell Transplantation
mesenchymal stem cells
neovascularization
Neovascularization, Physiologic - physiology
Osteogenesis - physiology
Rats
Skull - surgery
Surgical Flaps
Tissue Scaffolds
bone regeneration
bone tissue engineering
cell therapy
endothelial progenitor cells
mesenchymal stem cells
neovascularization
ISSN:1523-0899, 1708-8208, 1708-8208
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Bone formation relies on sufficient blood supply and osteoprogenitor cells. Purpose The study aims to evaluate the influence of endothelial progenitor cells (EPCs) in combination with mesenchymal stem cells (MSCs) on early vascularization and intramembranous bone regeneration. Materials and Methods Vertical bone regeneration was tested in rat calvarium guided bone regeneration model. Gold domes were filled with a mixture of 5 × 105 osteogenic transformed MSC and 5 × 105 EPC (EPC/MSC) that were mixed with β‐tricalcium phosphate (βTCP) scaffold. Domes filled with βTCP alone served as control. Rats were sacrificed after 4 or 12 weeks. Histomorphometry was used to determine blood vessel (Bv) density, vertical bone height, and bone area in the regenerated tissue. Results At both time points, new augmented hard tissue filled the space under the dome, and Bv density was higher in the EPC/MSC transplanted group vs control. However, bone height and bone area were similar among the groups 4 weeks posttransplantation, but were doubled in the EPC/MSC transplanted group 12 weeks posttransplantation. Conclusions EPC/MSC transplantation increases Bv formation in the early stages of healing that precedes enhancement of extracortical bone regeneration in later stages.
Bibliography:ark:/67375/WNG-DNS62M4S-J
Rambam Health Care Campus, Haifa, Israel
ArticleID:CID12104
Ministry of Industry Trade and Labor, Israel - No. 46293
istex:2041A80373FF66023847F964D532301462DA1B77
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1523-0899
1708-8208
1708-8208
DOI:10.1111/cid.12104