Essential Techniques for Medical and Life Scientists
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| Main Author: | |
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| Format: | eBook |
| Language: | English |
| Published: |
Sharjah
Bentham Science Publishers
2018
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| Edition: | 1 |
| Subjects: | |
| Online Access: | Get full text |
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Table of Contents:
- Intro -- CONTENTS -- FOREWORD -- PREFACE -- List of Contributors -- Application of Mass Spectrometry in Proteomics -- Serap Pektas* -- INTRODUCTION -- 1. INLET SYSTEMS -- 2. IONIZATION TECHNIQUE -- 2.1. Electrospray Ionization (ESI) -- 2.2. Matrix Assisted Laser Desorption Ionization (MALDI) -- 2.2.1. MALDI Sample Preparation -- 3. MASS ANALYZERS -- 3.1. Quadrupole Mass Analyzer -- Tandem Mass Spectrometry MS/MS -- 3.2. Ion Trap Mass Analyzer (IT) -- 3.3. Orbitrap Mass Analyzer -- 3.4. Time of Flight (TOF) Mass Analyzer -- 4. DETECTORS -- 5. APPLICATIONS -- 5.1. Proteomics -- 5.1.1. Molecular Weight Determination -- 5.1.2. Protein Sequencing -- 5.1.3. Post-Translational Modifications -- 5.1.4. Protein-Protein, Protein-DNA and Protein-Small Molecule Interactions -- 5.1.5. Protein Folding and Unfolding Rates -- 5.2. Microbiology -- 5.3. Medicine -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Structural Elucidation of Macromolecules -- Ana Luísa Carvalho, Teresa Santos-Silva, Maria João Romão, Eurico J. Cabrita and Filipa Marcelo* -- INTRODUCTION -- 2.1. Solving the 3D Structure of Proteins Using X-ray Crystallography -- 2.1.1. Protein Crystallization -- 2.1.2. Diffraction of X-rays by Protein Crystals -- 2.1.3. 3D Structure Solution and Refinement -- 2.1.4. Validation and Analysis of Crystal Structures -- 2.2. NMR Guide into Molecular Recognition -- 2.2.1. NMR and the Study of Intermolecular Interactions -- 2.2.2. Protein-detected NMR Based Experiments -- 2.2.3. Ligand-detected NMR Based Experiments -- 2.3. Studying Protein Shape and Protein-ligand Interactions by SAXS Methods -- 2.3.1. Data Collection -- 2.3.2. Preliminary Sample Characterization: Guinier -- Porod, Kratky and Pair Distribution Functions -- 2.3.3. Deriving a Structural Model -- 2.4. Structural Elucidation Using Cryo-Electron Microscopy
- INTRODUCTION -- 4.1. Methodology -- 4.1.1. Isolation of RNA -- 4.1.2. Reverse Transcription or Complementary DNA Synthesis (cDNA Synthesis) -- 4.1.3. PCR Amplification -- 4.2. Applications -- 4.3. Precautions to be Taken -- 4.3.1. Real-time Quantitative PCR (RT-qPCR) -- 4.4. Future Perspectives -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- SUBJECT INDEX
- 2.5. Notes -- 2.5.1. Growing and Preserving a Protein Crystal -- 2.5.2. Bragg's Law -- 2.5.3. Seleno-methionine Derivatives for Structure Solution -- 2.5.4. Patterson Maps and Molecular Replacement -- 2.5.5. Electron Density Maps -- 2.5.5. The Temperature Factor (B Factor) -- 2.5.6. The Ramachandran Plot -- 2.5.7. Technical Aspects of NMR Hardware and Software -- 2.5.8. NMR Sample -- 2.5.9. General Aspects on Acquisition and Processing of NMR Spectra -- 2.5.10. Basics on 15N-1H-HSQC Titrations -- 2.5.11. Basics on Transferred NOE -- 2.5.12. Basics on STD-NMR Experiments -- 2.5.13. Basics on Diffusion Experiments -- 2.5.14. SAXS Buffer Subtraction -- 2.5.15. Sample Quality for Successful BioSAXS Experiment -- 2.5.16. Radiation Damage -- 2.5.17. Small Angle Neutron Scattering (SANS) -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Isothermal Titration Calorimetry -- Ozlem Ustun Aytekin1, Elvan Yilmaz Akyuz1, Banu Bayram1, Esen Tutar2, Halime Hanım Pence3 and Yusuf Tutar1,4,* -- INTRODUCTION -- 3.1. ITC in Protein Science and Enzyme Technology -- 3.1.1. Protein-protein Interactions -- 3.1.2. Protein- DNA/RNA Interactions -- 3.1.3. Enzyme Kinetics -- 3.2. ITC in Pharmaceutical Research -- 3.2.1. ITC in Drug Delivery -- 3.2.2. ITC in Drug Screening -- 3.2.3. ITC in Drug Design -- 3.2.4. ITC in Drug Development -- 3.3. ITC Applications in Food Science -- 3.3.1. Interactions Involving Proteins or Peptides -- 3.3.2. Interactions Involving Carbohydrates -- 3.3.3. Interactions Involving Lipids -- 3.4. Protocol -- 3.4.1. Sample Preparation -- 3.4.2. Measurement -- 3.4.3. Data Collection and Analysis -- 3.5. Troubleshooting -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- Venkidasamy Baskar and Sathishkumar Ramalingam