Antisense Expression of the CK2 α-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because of its ability to phosphorylate transcription factors that bind to the promoter regions of light-regulated genes in vitro. To address this possibility in vivo and to lear...

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Veröffentlicht in:	Plant physiology (Bethesda) Jg. 119; H. 3; S. 989 - 1000
Hauptverfasser:	Yew Lee, Lloyd, Alan M., Roux, Stanley J.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Rockville, MD American Society of Plant Physiologists 01.03.1999 American Society of Plant Biologists
Schlagworte:	antisense DNA Arabidopsis Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis thaliana Base Sequence Biological and medical sciences Casein Kinase II CDC2 Protein Kinase CDC2 Protein Kinase - genetics CDC2 Protein Kinase - metabolism chemical constituents of plants DNA DNA, Antisense DNA, Antisense - genetics DNA-binding proteins enzymology Fundamental and applied biological sciences. Psychology Gene expression Gene expression regulation Gene Expression Regulation, Enzymologic Gene Expression Regulation, Enzymologic - radiation effects Gene Expression Regulation, Plant Gene Expression Regulation, Plant - radiation effects Genes Genes, Plant genetic transformation genetics growth & development leaf area Light metabolism Molecular and cellular biology Molecular genetics Molecular Genetics and Genomics phenotype Phosphorylation Physical agents Plant cells Plant growth Plant Leaves Plant Leaves - growth & development Plant physiology and development Plants Plants, Genetically Modified promoter regions Protein Serine-Threonine Kinases Protein Serine-Threonine Kinases - genetics radiation effects red light RNA RNA, Messenger RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant RNA, Plant - genetics RNA, Plant - metabolism Seedlings Signal transduction transcription factors Transformation, Genetic transgenic plants Vegetative apparatus, growth and morphogenesis. Senescence Light effect Phosphorylation Enzyme Growth Transferases Gene expression Arabidopsis thaliana Signal transduction Phenotype Regulation(control) Cruciferae Protein kinase Dicotyledones Light Angiospermae Phytochrome Spermatophyta Transgenic plant Antisense DNA Experimental plant Transcription factor Non-programmatic
ISSN:	0032-0889, 1532-2548
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Beschreibung
Zusammenfassung:	The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because of its ability to phosphorylate transcription factors that bind to the promoter regions of light-regulated genes in vitro. To address this possibility in vivo and to learn more about the potential physiological roles of CK2 in plants, we transformed Arabidopsis with an antisense construct of the CK2 α-subunit gene and investigated both morphological and molecular phenotypes. Antisense transformants had a smaller adult leaf size and showed increased expression of chs in darkness and of cab and rbcS after red-light treatment. The latter molecular phenotype implied that CK2 might serve as one of several negative and quantitative effectors in light-regulated gene expression. The possible mechanism of CK2 action and its involvement in the phytochrome signal transduction pathway are discussed.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 ObjectType-Feature-2 Corresponding author; e-mail sroux@uts.cc.utexas.edu; fax 1–512–471–3878.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.119.3.989