Modification of glycopeptidolipids by an O-methyltransferase of Mycobacterium smegmatis

Glycopeptidolipids (GPLs) are a major component of the outer layers of the cell walls of several non-tuberculous mycobacteria. The Mycobacterium smegmatis GPLs consist of a diglycosylated lipopeptide core which is variably modified by acetylation and methylation. Analysis of a region of the M. smegm...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) Vol. 148; no. Pt 10; p. 3079
Main Authors: Jeevarajah, Dharshini, Patterson, John H, McConville, Malcolm J, Billman-Jacobe, Helen
Format: Journal Article
Language:English
Published: England 01.10.2002
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Gas Chromatography-Mass Spectrometry
Gene Deletion
Genetic Complementation Test
Glycolipids - chemistry
Glycolipids - metabolism
Glycopeptides - chemistry
Glycopeptides - metabolism
Methylation
Methyltransferases - genetics
Methyltransferases - metabolism
Molecular Sequence Data
Multigene Family
Mycobacterium smegmatis - enzymology
Mycobacterium smegmatis - genetics
Sequence Analysis, DNA
Spectrometry, Mass, Electrospray Ionization
ISSN:1350-0872
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Summary:Glycopeptidolipids (GPLs) are a major component of the outer layers of the cell walls of several non-tuberculous mycobacteria. The Mycobacterium smegmatis GPLs consist of a diglycosylated lipopeptide core which is variably modified by acetylation and methylation. Analysis of a region of the M. smegmatis chromosome, upstream of the peptide synthetase gene, mps, revealed a GPL biosynthetic locus containing genes potentially involved in glycosylation, methylation, acetylation and transport of GPLs. Methyltransferases are required to modify rhamnose and the fatty acid of GPLs. Of the four methyltransferases encoded within the locus, one methyltransferase, Mtf2, was unlike sugar methyltransferases from other species. An mtf2 mutant was created and was shown to be unable to methylate the GPL fatty acids. Direct evidence is presented that Mtf2 is a methyltransferase that modifies the GPL fatty acid.
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ISSN:1350-0872
DOI:10.1099/00221287-148-10-3079