Methods for Bacteriophage Preservation

In a view of growing interest in bacteriophages as the most abundant members of microbial communities and as antibacterial agents, reliable methods for bacteriophage long-term preservation, that warrant the access to original or mutant stocks of unchanged properties, have become of crucial importanc...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1693; p. 219
Main Authors: Łobocka, Małgorzata B, Głowacka, Aleksandra, Golec, Piotr
Format: Journal Article
Language:English
Published: United States 2018
Subjects:
Bacteria - virology
Bacteriophages - physiology
Preservation, Biological - methods
Virion - physiology
Bacteria
Microbiological methods
Bacteriophage preservation
Deep-freezing
Bacteriophage storage
Bacteriophage stability
ISSN:1940-6029, 1940-6029
Online Access:Get more information
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Summary:In a view of growing interest in bacteriophages as the most abundant members of microbial communities and as antibacterial agents, reliable methods for bacteriophage long-term preservation, that warrant the access to original or mutant stocks of unchanged properties, have become of crucial importance. A storage method that retains the infectivity of any kind of bacteriophage virions, either in a cell lysate or in a purified suspension, does not exist, due to the enormous diversity of bacteriophages and hence the differentiation of their sensitivity to various storage conditions. Here, we describe a method of long-term bacteriophage preservation, which is based on freezing of freshly infected susceptible bacteria at early stages of bacteriophage development. The infected bacteria release mature bacteriophages upon melting enabling the recovery of bacteriophage virions with high efficiency. The only limitation of this method is the sensitivity of bacteriophage host to deep-freezing, and thus it can be used for the long-term preservation of the vast majority of bacteriophages.
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ISSN:1940-6029
1940-6029
DOI:10.1007/978-1-4939-7395-8_17