Evaluation of Leuconostoc mesenteroides YML003 as a probiotic against low‐pathogenic avian influenza (H9N2) virus in chickens

Aim: The aims of the study were to isolate anti‐H9N2 bacteria from Korean Kimchi isolates and to evaluate its performance in cell line, egg and in specific pathogen‐free (SPF) chickens. Methods and Results: Using Madin–Darby canine kidney (MDCK) cell line, 220 bacterial isolates were screened and th...

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Vydané v:	Journal of applied microbiology Ročník 113; číslo 1; s. 163 - 171
Hlavní autori:	Seo, B.J, Rather, I.A, Kumar, V.J.R, Choi, U.H, Moon, M.R, Lim, J.H, Park, Y.H
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Oxford, UK Blackwell Publishing Ltd 01.07.2012 Blackwell Oxford University Press
Predmet:	Animals antibiotic resistance antiviral activity antiviral properties Avian flu avian influenza Avian influenza virus bacteria bile acids Biological and medical sciences Body weight Brassica - microbiology chicken probiotics Chickens Chickens - virology Cloaca - virology Dogs eggs Fundamental and applied biological sciences. Psychology gastric juice Genes hemagglutination Hemagglutination Tests immunomodulating Influenza A virus Influenza A Virus, H9N2 Subtype - pathogenicity Influenza in Birds - prevention & control interferon-gamma Interferon-gamma - metabolism kidneys kimchi Leuconostoc Leuconostoc - genetics Leuconostoc - isolation & purification Leuconostoc mesenteroides low-pathogenic avian influenza (H9N2) virus Madin Darby Canine Kidney Cells Microbiology Ovum - virology Poultry poultry industry Probiotics Probiotics - pharmacology Reverse Transcriptase Polymerase Chain Reaction ribosomal RNA RNA, Ribosomal, 16S - genetics specific pathogen-free animals Specific Pathogen-Free Organisms Spleen - immunology Trachea - virology Vegetables - microbiology viruses Probiotic Leuconostoc mesenteroides chicken probiotics Lactic acid bacteria low-pathogenic avian influenza (H9N2) virus Immunomodulation Applied microbiology Orthomyxoviridae antiviral activity immunomodulating Virus Streptococcaceae Vertebrata Influenzavirus A Avian influenzavirus Pathogenic Bacteria Micrococcales Antiviral Chicken Aves
ISSN:	1364-5072, 1365-2672, 1365-2672
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Shrnutí:	Aim: The aims of the study were to isolate anti‐H9N2 bacteria from Korean Kimchi isolates and to evaluate its performance in cell line, egg and in specific pathogen‐free (SPF) chickens. Methods and Results: Using Madin–Darby canine kidney (MDCK) cell line, 220 bacterial isolates were screened and the isolate YML003 was selected having pronounced antiviral activity against H9N2 virus. This isolate was identified as Leuconostoc mesenteroides by 16S rRNA gene sequencing. Anti‐H9N2 activity of the strain was also evaluated by hemagglutination assay. Leuconostoc mesenteroides YML003 was assessed for its survival in gastric juice and 5% bile acid and the antibiotic susceptibility. Both live and heat‐killed cells were selected for in vivo chicken feeding experiment. Body weight, immune index, serobiochemical parameters and splenic IFN‐γ production were assessed during selected intervals. Viral population in the trachea and cloacae were calculated by quantitative real‐time reverse transcriptase PCR (qRT‐PCR). Conclusions: Leuconostoc mesenteroides YML003 exhibited anti‐H9N2 activity both in in vitro cell line as well as in vivo SPF Chickens. Significance and Impact of the Study: This is a primary report on the anti‐H9N2 activity by a Leuconostoc strain. Amid the increasing reports of avian influenza virus occurrence resulting in severe losses to the poultry industry, prophylactic administration of such probiotic strains are highly significant.
Bibliografia:	http://dx.doi.org/10.1111/j.1365-2672.2012.05326.x istex:34E026535FFF56DA255E6653ADE5015CC075742A ArticleID:JAM5326 ark:/67375/WNG-40H0WDGC-D SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5072 1365-2672 1365-2672
DOI:	10.1111/j.1365-2672.2012.05326.x