Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections

Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish a...

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Vydáno v:Veterinary research (Paris) Ročník 26; číslo 5-6; s. 499
Hlavní autoři: Falk, K, Dannevig, B H
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 1995
Témata:
Anemia - diagnosis
Anemia - pathology
Anemia - veterinary
Animals
Antibodies
Antibodies, Monoclonal
Antigens, Viral - analysis
Cell Line
Endothelium, Vascular - pathology
Endothelium, Vascular - virology
Fish Diseases
Fluorescent Antibody Technique, Indirect
Heart - virology
Kidney
Lentivirus Infections - diagnosis
Lentivirus Infections - pathology
Lentivirus Infections - veterinary
Mice
Mice, Inbred BALB C - immunology
Myocardium - pathology
Rabbits - immunology
Salmon - virology
ISSN:0928-4249
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Shrnutí:Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
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ISSN:0928-4249