Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections

Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish a...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary research (Paris) Vol. 26; no. 5-6; p. 499
Main Authors: Falk, K, Dannevig, B H
Format: Journal Article
Language:English
Published: England 1995
Subjects:
Anemia - diagnosis
Anemia - pathology
Anemia - veterinary
Animals
Antibodies
Antibodies, Monoclonal
Antigens, Viral - analysis
Cell Line
Endothelium, Vascular - pathology
Endothelium, Vascular - virology
Fish Diseases
Fluorescent Antibody Technique, Indirect
Heart - virology
Kidney
Lentivirus Infections - diagnosis
Lentivirus Infections - pathology
Lentivirus Infections - veterinary
Mice
Mice, Inbred BALB C - immunology
Myocardium - pathology
Rabbits - immunology
Salmon - virology
ISSN:0928-4249
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
AbstractList Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
Author Falk, K
Dannevig, B H
Author_xml – sequence: 1
  givenname: K
  surname: Falk
  fullname: Falk, K
  organization: Department of Virology, Central Veterinary Laboratory, Oslo, Norway
– sequence: 2
  givenname: B H
  surname: Dannevig
  fullname: Dannevig, B H
BackLink https://www.ncbi.nlm.nih.gov/pubmed/8581029$$D View this record in MEDLINE/PubMed
BookMark eNotkM1OwzAQhH0oKm3hEZB8QnCIZDtO7Byr8lepEgfgHDnxGhklTvE6SLw9Bnpa7c6n0cyuySJMARZkxRqhCylkc07WiB-M8bqs5JIsdaU5E82K2DsYp4ApmuSnQCdHfXDQ52VGimbIIjXBwOgNvdm_bG_pl49myLfk3yFgxmkPw0D7eUhzBMyKpckjzkDxzyjgBTlzZkC4PM0NeXu4f909FYfnx_1ueyic0FUqjFLQMNcpJ0XVSMM5yzmtZg4q16te29qWkGPXVlpV1hwAelsK1UkleGPFhlz_-x7j9DkDpnb0-JvOBMh9WqVqKZVWGbw6gXM3gm2P0Y8mfrent4gfti5gVA
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Veterinary Medicine
ExternalDocumentID 8581029
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
--K
-A0
0R~
123
1B1
2VQ
2WC
3V.
4.4
53G
5VS
7X7
88E
88I
8FI
8FJ
AAFWJ
AAHBH
AAJSJ
AAOTM
ABUBZ
ABUWG
ACGFS
ACGOD
ACRMQ
ADBBV
ADINQ
ADRAZ
ADUKV
AENEX
AFKRA
AFPKN
AHBYD
AHMBA
AHSBF
AHYZX
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMKLP
AOIJS
AZQEC
BAWUL
BCNDV
BENPR
BFQNJ
BMC
BPHCQ
BVXVI
C24
C6C
CCPQU
CGR
CS3
CUY
CVF
DIK
DU5
DWQXO
E3Z
EBD
EBLON
EBS
ECGQY
ECM
EIF
EJD
EMOBN
EYRJQ
F5P
FRP
FYUFA
GI~
GNUQQ
GROUPED_DOAJ
H13
HCIFZ
HMCUK
HYE
IAG
IAO
IHE
INH
INR
IPNFZ
ITC
KQ8
M1P
M2P
M41
M48
M~E
NPM
NQ-
O5R
O5S
OK1
PGMZT
PIMPY
PQQKQ
PROAC
PSQYO
RBZ
RED
RIG
RNS
ROL
RPM
RPZ
RSV
SMD
SOJ
SV3
TR2
UKHRP
7X8
ID FETCH-LOGICAL-f285t-a77e90fb7f42594a110858d80fe5fc7c8d6d3e0296d4d7361eeecd327b47219d2
IEDL.DBID 7X8
ISICitedReferencesCount 40
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=3674051&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 0928-4249
IngestDate Thu Sep 04 17:13:11 EDT 2025
Sat Sep 28 07:31:46 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 5-6
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-f285t-a77e90fb7f42594a110858d80fe5fc7c8d6d3e0296d4d7361eeecd327b47219d2
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 8581029
PQID 77644787
PQPubID 23479
ParticipantIDs proquest_miscellaneous_77644787
pubmed_primary_8581029
PublicationCentury 1900
PublicationDate 1995-00-00
19950101
PublicationDateYYYYMMDD 1995-01-01
PublicationDate_xml – year: 1995
  text: 1995-00-00
PublicationDecade 1990
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Veterinary research (Paris)
PublicationTitleAlternate Vet Res
PublicationYear 1995
SSID ssj0016354
Score 1.583002
Snippet Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 499
SubjectTerms Anemia - diagnosis
Anemia - pathology
Anemia - veterinary
Animals
Antibodies
Antibodies, Monoclonal
Antigens, Viral - analysis
Cell Line
Endothelium, Vascular - pathology
Endothelium, Vascular - virology
Fish Diseases
Fluorescent Antibody Technique, Indirect
Heart - virology
Kidney
Lentivirus Infections - diagnosis
Lentivirus Infections - pathology
Lentivirus Infections - veterinary
Mice
Mice, Inbred BALB C - immunology
Myocardium - pathology
Rabbits - immunology
Salmon - virology
Title Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections
URI https://www.ncbi.nlm.nih.gov/pubmed/8581029
https://www.proquest.com/docview/77644787
Volume 26
WOSCitedRecordID wos3674051&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV05T8MwFH4qlIGFo1BRTg8MMETkti0hoQqoQKJVJQ51ixwfUqU2LaTw-3l2ErHBwJIhjiPHz3nvezfAuaKIilUUeQGXqKCECfPy1JeepCGqG3GAs1zJ_Cc6GrHJhI9bcN3kwtiwyoYnOkatFtLayK8oRcmNp-tm-e7ZnlHWt1o30FiDdoRAxgZ00cmPDwFFqSsexa39KIx_wZBOlgy2_7eKHdiqMSTpV0TfhZYuOtB5s4EtLruWDGuH-R6oOz23CLCiM1kY0kRffZakFDMcJKIQej4V5OLxuX9JbNDvDO-tbJnOEh8n1rZPqgodusQRRVaOWqR0LyrKfXgd3L_cPnh1YwXPhCxZeYJSzX2TU4PE4bGwqQAJU8w3OjGSSqaQfNoPeapiRaM00FpLFYU0j1Fh5CrswnqxKPQBkDxgeYqoUkRMxNbLhiwgSJFpmYiliuc9OGs2MsODa1csCo3fmDVb2YNuRYtsWdXXyHAtCHv44Z9Tj2CzyjC3FpFjaBv8Y_UJbMgv3IePU3cc8DoaD78BEDfDNw
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Demonstration+of+infectious+salmon+anaemia+%28ISA%29+viral+antigens+in+cell+cultures+and+tissue+sections&rft.jtitle=Veterinary+research+%28Paris%29&rft.au=Falk%2C+K&rft.au=Dannevig%2C+B+H&rft.date=1995-01-01&rft.issn=0928-4249&rft.volume=26&rft.issue=5-6&rft.spage=499&rft_id=info%3Apmid%2F8581029&rft_id=info%3Apmid%2F8581029&rft.externalDocID=8581029
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0928-4249&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0928-4249&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0928-4249&client=summon