A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2
The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respir...
Uloženo v:
| Vydáno v: | Cell Ročník 183; číslo 1; s. 169 |
|---|---|
| Hlavní autoři: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
01.10.2020
|
| Témata: | |
| ISSN: | 1097-4172, 1097-4172 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and mice expressing the human angiotensin-converting enzyme 2 receptor. Intramuscular dosing of ChAd-SARS-CoV-2-S induces robust systemic humoral and cell-mediated immune responses and protects against lung infection, inflammation, and pathology but does not confer sterilizing immunity, as evidenced by detection of viral RNA and induction of anti-nucleoprotein antibodies after SARS-CoV-2 challenge. In contrast, a single intranasal dose of ChAd-SARS-CoV-2-S induces high levels of neutralizing antibodies, promotes systemic and mucosal immunoglobulin A (IgA) and T cell responses, and almost entirely prevents SARS-CoV-2 infection in both the upper and lower respiratory tracts. Intranasal administration of ChAd-SARS-CoV-2-S is a candidate for preventing SARS-CoV-2 infection and transmission and curtailing pandemic spread. |
|---|---|
| AbstractList | The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and mice expressing the human angiotensin-converting enzyme 2 receptor. Intramuscular dosing of ChAd-SARS-CoV-2-S induces robust systemic humoral and cell-mediated immune responses and protects against lung infection, inflammation, and pathology but does not confer sterilizing immunity, as evidenced by detection of viral RNA and induction of anti-nucleoprotein antibodies after SARS-CoV-2 challenge. In contrast, a single intranasal dose of ChAd-SARS-CoV-2-S induces high levels of neutralizing antibodies, promotes systemic and mucosal immunoglobulin A (IgA) and T cell responses, and almost entirely prevents SARS-CoV-2 infection in both the upper and lower respiratory tracts. Intranasal administration of ChAd-SARS-CoV-2-S is a candidate for preventing SARS-CoV-2 infection and transmission and curtailing pandemic spread.The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and mice expressing the human angiotensin-converting enzyme 2 receptor. Intramuscular dosing of ChAd-SARS-CoV-2-S induces robust systemic humoral and cell-mediated immune responses and protects against lung infection, inflammation, and pathology but does not confer sterilizing immunity, as evidenced by detection of viral RNA and induction of anti-nucleoprotein antibodies after SARS-CoV-2 challenge. In contrast, a single intranasal dose of ChAd-SARS-CoV-2-S induces high levels of neutralizing antibodies, promotes systemic and mucosal immunoglobulin A (IgA) and T cell responses, and almost entirely prevents SARS-CoV-2 infection in both the upper and lower respiratory tracts. Intranasal administration of ChAd-SARS-CoV-2-S is a candidate for preventing SARS-CoV-2 infection and transmission and curtailing pandemic spread. The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and mice expressing the human angiotensin-converting enzyme 2 receptor. Intramuscular dosing of ChAd-SARS-CoV-2-S induces robust systemic humoral and cell-mediated immune responses and protects against lung infection, inflammation, and pathology but does not confer sterilizing immunity, as evidenced by detection of viral RNA and induction of anti-nucleoprotein antibodies after SARS-CoV-2 challenge. In contrast, a single intranasal dose of ChAd-SARS-CoV-2-S induces high levels of neutralizing antibodies, promotes systemic and mucosal immunoglobulin A (IgA) and T cell responses, and almost entirely prevents SARS-CoV-2 infection in both the upper and lower respiratory tracts. Intranasal administration of ChAd-SARS-CoV-2-S is a candidate for preventing SARS-CoV-2 infection and transmission and curtailing pandemic spread. |
| Author | Keeler, Shamus P Weiskopf, Daniela Ma, Meisheng Hou, Yixuan J Kafai, Natasha M VanBlargan, Laura A Smith, Brittany K Fox, Julie M Holtzman, Michael J Bailey, Adam L Curiel, David T Hassan, Ahmed O Kim, Arthur S Harvey, Ian B Zhao, Haiyan Gralinski, Lisa E Schäfer, Alexandra Dmitriev, Igor P Dai, Ya-Nan McCune, Broc T Wessel, Alex W Chen, Rita E Adams, Lucas J Shrihari, Swathi Kashentseva, Elena Baric, Ralph S Danis, Jonathan E Fremont, Daved H Case, James Brett Diamond, Michael S Winkler, Emma S |
| Author_xml | – sequence: 1 givenname: Ahmed O surname: Hassan fullname: Hassan, Ahmed O organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 2 givenname: Natasha M surname: Kafai fullname: Kafai, Natasha M organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 3 givenname: Igor P surname: Dmitriev fullname: Dmitriev, Igor P organization: Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 4 givenname: Julie M surname: Fox fullname: Fox, Julie M organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 5 givenname: Brittany K surname: Smith fullname: Smith, Brittany K organization: Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 6 givenname: Ian B surname: Harvey fullname: Harvey, Ian B organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 7 givenname: Rita E surname: Chen fullname: Chen, Rita E organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 8 givenname: Emma S surname: Winkler fullname: Winkler, Emma S organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 9 givenname: Alex W surname: Wessel fullname: Wessel, Alex W organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 10 givenname: James Brett surname: Case fullname: Case, James Brett organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 11 givenname: Elena surname: Kashentseva fullname: Kashentseva, Elena organization: Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 12 givenname: Broc T surname: McCune fullname: McCune, Broc T organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 13 givenname: Adam L surname: Bailey fullname: Bailey, Adam L organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 14 givenname: Haiyan surname: Zhao fullname: Zhao, Haiyan organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 15 givenname: Laura A surname: VanBlargan fullname: VanBlargan, Laura A organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 16 givenname: Ya-Nan surname: Dai fullname: Dai, Ya-Nan organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 17 givenname: Meisheng surname: Ma fullname: Ma, Meisheng organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 18 givenname: Lucas J surname: Adams fullname: Adams, Lucas J organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 19 givenname: Swathi surname: Shrihari fullname: Shrihari, Swathi organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 20 givenname: Jonathan E surname: Danis fullname: Danis, Jonathan E organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 21 givenname: Lisa E surname: Gralinski fullname: Gralinski, Lisa E organization: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA – sequence: 22 givenname: Yixuan J surname: Hou fullname: Hou, Yixuan J organization: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA – sequence: 23 givenname: Alexandra surname: Schäfer fullname: Schäfer, Alexandra organization: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA – sequence: 24 givenname: Arthur S surname: Kim fullname: Kim, Arthur S organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 25 givenname: Shamus P surname: Keeler fullname: Keeler, Shamus P organization: Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 26 givenname: Daniela surname: Weiskopf fullname: Weiskopf, Daniela organization: Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA – sequence: 27 givenname: Ralph S surname: Baric fullname: Baric, Ralph S organization: Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA – sequence: 28 givenname: Michael J surname: Holtzman fullname: Holtzman, Michael J organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 29 givenname: Daved H surname: Fremont fullname: Fremont, Daved H organization: Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA – sequence: 30 givenname: David T surname: Curiel fullname: Curiel, David T email: dcuriel@wustl.edu organization: Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: dcuriel@wustl.edu – sequence: 31 givenname: Michael S surname: Diamond fullname: Diamond, Michael S email: diamond@wusm.wustl.edu organization: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: diamond@wusm.wustl.edu |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32931734$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkLtOwzAYRi1URC_wAgzII0uC7dhOMkblVqkSqC1dI8f-U1KldrBTob49rSgS03eGo284YzSwzgJCt5TElFD5sI01tG3MCCMxyWLC5AUaUZKnEacpG_zjIRqHsCWEZEKIKzRMWJ7QNOEjZAq8bOymhejRBcAz23tlVVAtnn4WBq-V1o0F_O5dD7oP-KPrwGNlDZ677yMtIHSNV73zB7zy6qSojWps6PGyWCyjqVtH7Bpd1qoNcHPeCVo9P62mr9H87WU2LeYR8Jz0UZ2qqgJR60wknNdECplmtZQqzWktapNDxU1dcZVxRQzLtSRpxaHSzIAmlE3Q_e9t593XHkJf7ppwSqQsuH0oGeeJoFJScVTvzuq-2oEpO9_slD-Uf2HYD0imZ88 |
| ContentType | Journal Article |
| Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
| Copyright_xml | – notice: Copyright © 2020 Elsevier Inc. All rights reserved. |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.cell.2020.08.026 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1097-4172 |
| ExternalDocumentID | 32931734 |
| Genre | Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NIAID NIH HHS grantid: 75N93019C00062 – fundername: NIAID NIH HHS grantid: T32 AI007163 – fundername: NIAID NIH HHS grantid: R01 AI127828 – fundername: NIAID NIH HHS grantid: F30 AI152327 – fundername: NIAID NIH HHS grantid: U01 AI149644 – fundername: NIAID NIH HHS grantid: HHSN272201200026C – fundername: NIAID NIH HHS grantid: R01 AI130591 – fundername: NIGMS NIH HHS grantid: T32 GM007200 – fundername: NHLBI NIH HHS grantid: R35 HL145242 – fundername: NIAID NIH HHS grantid: F32 AI138392 |
| GroupedDBID | --- --K -DZ -ET -~X 0R~ 0WA 1RT 1~5 29B 2FS 2WC 3EH 4.4 457 4G. 53G 5GY 5RE 62- 6J9 7-5 85S AAEDT AAEDW AAFWJ AAHBH AAKRW AAKUH AALRI AAMRU AAVLU AAXUO AAYWO ABCQX ABDGV ABJNI ABMAC ABOCM ACGFO ACGFS ACNCT ACVFH ADBBV ADCNI ADEZE ADVLN ADXHL AEFWE AENEX AEUPX AEXQZ AFPUW AFTJW AGCQF AGHSJ AGKMS AHHHB AIGII AITUG AKAPO AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ APXCP ASPBG AVWKF AZFZN BAWUL CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EFKBS EIF F5P FCP FDB FIRID HH5 IH2 IHE IXB J1W JIG K-O KOO KQ8 L7B LX5 M3Z M41 N9A NPM O-L O9- OK1 P2P RNS ROL RPZ SCP SDG SDP SES SSZ TAE TN5 TR2 TWZ UKR UPT WH7 YZZ ZCA 7X8 |
| ID | FETCH-LOGICAL-e490t-f7abbe5fc85344f065678f66a791f5fd9eb4dfb4a84a0d29c607b4ebc2dec012 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 432 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000576772900015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1097-4172 |
| IngestDate | Thu Oct 02 11:28:43 EDT 2025 Mon Jul 21 05:50:58 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | COVID-19 pathogenesis IgA SARS-CoV-2 vaccine antibody intranasal protection T cells mucosal immunity |
| Language | English |
| License | Copyright © 2020 Elsevier Inc. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-e490t-f7abbe5fc85344f065678f66a791f5fd9eb4dfb4a84a0d29c607b4ebc2dec012 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://www.cell.com/article/S0092867420310680/pdf |
| PMID | 32931734 |
| PQID | 2443516615 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2443516615 pubmed_primary_32931734 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-10-01 |
| PublicationDateYYYYMMDD | 2020-10-01 |
| PublicationDate_xml | – month: 10 year: 2020 text: 2020-10-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Cell |
| PublicationTitleAlternate | Cell |
| PublicationYear | 2020 |
| SSID | ssj0008555 |
| Score | 2.7178218 |
| Snippet | The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 169 |
| SubjectTerms | Adenoviridae - genetics Administration, Intranasal Animals Antibodies, Neutralizing - blood Antibodies, Viral - blood Chlorocebus aethiops Coronavirus Infections - immunology Coronavirus Infections - pathology Coronavirus Infections - prevention & control COVID-19 COVID-19 Vaccines Female HEK293 Cells Humans Immunogenicity, Vaccine Injections, Intramuscular Mice Mice, Inbred BALB C Pandemics Pneumonia, Viral - immunology Pneumonia, Viral - pathology Respiratory Mucosa - immunology Respiratory Mucosa - pathology Respiratory Mucosa - virology Spike Glycoprotein, Coronavirus - genetics Spike Glycoprotein, Coronavirus - immunology Vero Cells Viral Vaccines - administration & dosage Viral Vaccines - immunology |
| Title | A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2 |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32931734 https://www.proquest.com/docview/2443516615 |
| Volume | 183 |
| WOSCitedRecordID | wos000576772900015&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bS8MwFA7qFHzxfpk3Ivga7NKkXZ5kTIfCHGObY28jVx1IW9cp-O89aTv2JAi-9C1Qck7yfeeS8yF0I8GHBHcQm4SCE_8UkgjdsCSkWjIuaJO7wtLduNdrTiaiXyXc8qqtcnknFhe1SbXPkd8CDIW8AWjC77IP4lWjfHW1ktBYR7UQqIz36niymhbe5IXqqS-yEgZIXT2aKfu7fGIc4kMaFCM8afQ7xSygprP735_cQzsVycSt0iv20ZpNDtBWKTv5fYhMCw8Bsd4tuU9zi598fjeROaxov7UMHkvtq-24X45wyPFLltk5lonBXa-phger8jwe-VdWOZavcgZEEw9bgyFpp2NCj9Co8zBqP5JKbYFYJoIFcbFUyvqWLh4y5oCaAI65KJKxaDjujLCKGaeYbDIZGCp0FMSKWaWpsRpg7hhtJGliTxFWNpLSAjGkmjNqhFRxQF3IIR6ODVWujq6XuzcFZ_aGkIlNP_Ppav_q6KQ0wTQrp25MQyAmjThkZ39YfY62vWXLprsLVHNwlO0l2tRfi1k-vyq8BL69_vMPBSbF1Q |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Single-Dose+Intranasal+ChAd+Vaccine+Protects+Upper+and+Lower+Respiratory+Tracts+against+SARS-CoV-2&rft.jtitle=Cell&rft.au=Hassan%2C+Ahmed+O&rft.au=Kafai%2C+Natasha+M&rft.au=Dmitriev%2C+Igor+P&rft.au=Fox%2C+Julie+M&rft.date=2020-10-01&rft.issn=1097-4172&rft.eissn=1097-4172&rft.volume=183&rft.issue=1&rft.spage=169&rft_id=info:doi/10.1016%2Fj.cell.2020.08.026&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-4172&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-4172&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-4172&client=summon |