Optimization of ion-exchange displacement separations: I. Validation of an iterative scheme and its use as a methods development tool

Displacement chromatography has been demonstrated to be a powerful, high-resolution preparative tool. The performance of displacement systems can be affected by a variety of factors such as the feed load, flow-rate, initial salt concentration and the displacer partition ratio. Thus, the optimization...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 876; no. 1; pp. 51 - 62
Main Authors:	Natarajan, Venkatesh, Wayne Bequette, B., Cramer, Steven M.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 21.04.2000 Elsevier
Subjects:	Algorithms Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Ion Exchange - methods Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Other chromatographic methods Proteins Proteins - analysis Quality Control Reproducibility of Results Salts - chemistry Displacement chromatography Steric mass action Ion-exchange chromatography Optimization Hemoprotein Phase composition Theoretical study Mobile phase Iterative program Displacement Protein Mass transfer Ion exchange chromatography Operating conditions Cytochrome c Salt effect Ion exchanger Numerical simulation
ISSN:	0021-9673
Online Access:	Get full text
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