Comparative analysis of human and mouse 3′ Igh regulatory regions identifies distinctive structural features

Immunoglobulin heavy chain ( Igh) locus rearrangements are controlled in part by an ∼30 kb complex 3′ regulatory region located 3′ of Cα: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3′ regulatory region and further downstream sequences from m...

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Bibliographic Details
Published in:Molecular immunology Vol. 42; no. 5; pp. 605 - 615
Main Authors: Sepulveda, Manuel A., Garrett, Francine E., Price-Whelan, Alexa, Birshtein, Barbara K.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.03.2005
Subjects:
3' Untranslated Regions
3′ Untranslated regions/immunology
Animals
B-lymphocytes/immunology
Base Sequence
Chromosomes, Artificial, Bacterial - genetics
Conserved sequence
DNA - genetics
Enhancer elements (genetics)/immunology
Evolution
Evolution, Molecular
Gene Rearrangement, B-Lymphocyte
Genes, Immunoglobulin
Genes, Regulator
Heavy-chain/genetics
Human
Humans
Immunoglobulins
Mice
Molecular
Molecular sequence data
Multigene Family
Nucleic acid
Pan troglodytes
Phylogeny
Polymorphism, Restriction Fragment Length
Rats
Regulatory sequences
Repetitive Sequences, Nucleic Acid
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity
Enhancer elements (genetics)/immunology
Human
3′ Untranslated regions/immunology
Immunoglobulins
Rats
Regulatory sequences
B-lymphocytes/immunology
Phylogeny
Molecular
Conserved sequence
Heavy-chain/genetics
Molecular sequence data
Evolution
Mice
Nucleic acid
ISSN:0161-5890, 1872-9142
Online Access:Get full text
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Description
Summary:Immunoglobulin heavy chain ( Igh) locus rearrangements are controlled in part by an ∼30 kb complex 3′ regulatory region located 3′ of Cα: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3′ regulatory region and further downstream sequences from mouse, rat, human and chimpanzee. Only short segments of homology were detected in the 3′ regulatory region, and these were located in the vicinity of the known 3′ enhancers. The nearest highly conserved segment is the nearest non- Igh gene, hole, which is located ∼62 kb downstream of mouse Cα. Analysis of murine 3′ Igh sequences by single nucleotide polymorphism (SNP) and restriction fragment length polymorphism (RFLP) detected a transition region (high to low SNP or RFLP density) ∼120 kb downstream of mouse Cα. Although there is only limited sequence identity between rodent and primate 3′ Igh regulatory regions, all of these regulatory regions contain a palindrome and locally repetitive elements. Locally repetitive elements in primates comprise blocks of “switch-like” sequences that differ from the families of inverted and tandem repeats that are present in rodents. We propose that together with enhancers, these “conserved” structural features are essential for the activity of the 3′ Igh regulatory region in vivo.
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ISSN:0161-5890
1872-9142
DOI:10.1016/j.molimm.2004.09.006