Proapoptotic and Antimigratory Effects of Pseudevernia furfuracea and Platismatia glauca on Colon Cancer Cell Lines

The aim of this study is to investigate cytotoxic, proapoptotic, antimigratory and pro-antioxidant effects of methanol, acetone and ethyl acetate extracts of lichens and on colorectal cancer (HCT-116 and SW-480) cell lines. We compared the cytotoxic effects on colorectal cancer cells with the effect...

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Vydáno v:Food technology and biotechnology Ročník 56; číslo 3; s. 421 - 430
Hlavní autoři: Šeklić, Dragana S, Obradović, Ana D, Stanković, Milan S, Živanović, Marko N, Mitrović, Tatjana Lj, Stamenković, Slaviša M, Marković, Snežana D
Médium: Journal Article
Jazyk:angličtina
Vydáno: Croatia Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet 01.09.2018
University of Zagreb Faculty of Food Technology and Biotechnology
Témata:
Acetic acid
Acetone
Acridine orange
Anticancer properties
Antioxidants
Antitumor activity
Apoptosis
Biotechnology
Cancer
Cancer therapies
Cell adhesion & migration
Cell migration
cell totility
Colon
Colon cancer
Colorectal cancer
Colorectal carcinoma
cytotoxic effects
Cytotoxicity
Ethidium bromide
Ethyl acetate
Fibroblasts
Fluorescence
Leukemia
lichen extracts
Lichens
Melanoma
Metabolites
Methanol
Original Scientific Papers
Oxidative stress
Physiology
Platismatia glauca
Pseudevernia furfuracea
redox status
Spectrophotometry
Toxicity
Tumor cell lines
Serbia
United States > US
Netherlands
cell motility
apoptosis
lichen extracts
colorectal cancer
redox status
cytotoxic effects
ISSN:1330-9862, 1334-2606
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Shrnutí:The aim of this study is to investigate cytotoxic, proapoptotic, antimigratory and pro-antioxidant effects of methanol, acetone and ethyl acetate extracts of lichens and on colorectal cancer (HCT-116 and SW-480) cell lines. We compared the cytotoxic effects on colorectal cancer cells with the effects obtained from normal human fibroblast (MRC-5) cell line. Tetrazolium (MTT) test evaluated the cytotoxic effects, Transwell assay evaluated cell migration, acridine orange/ethidium bromide (AO/EB) fluorescent method followed the apoptosis, while prooxidant/antioxidant effects were determined spectrophotometrically through concentration of redox parameters. The tested extracts showed considerable cytotoxic effect on cancer cells with no observable cytotoxic effect on normal cells. Ethyl acetate and acetone extract of induced the highest cytotoxicity (IC =(21.2±1.3) µg/mL on HCT-116, and IC =(51.3±0.8) µg/mL on SW-480 cells, respectively, after 72 h), with noteworthy apoptotic and prooxidant effects, and antimigratory potential of methanol extract. extracts induced cytotoxic effects on HCT-116 cells after 72 h (IC <40 μg/mL), while only methanol and acetone extracts had cytotoxic effects on SW-480 cells after 24 h, with proapoptotic/necrotic activity, as a consequence of induced oxidative stress. In conclusion, lichen extracts changed to a great extent cell viability and migratory potential of colorectal cancer cell lines. HCT-116 cells were more sensitive to treatments, had better proapoptotic and antimigratory effects, and both investigated lichen species might be a source of substances with anticancer activity.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1330-9862
1334-2606
DOI:10.17113/ftb.56.03.18.5727