Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells
ABSTRACT Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated...
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| Vydáno v: | Pesquisa brasileira em odontopediatria e clínica integrada Ročník 24 |
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Associação de Apoio à Pesquisa em Saúde Bucal
2024
Association of Support to Oral Health Research (APESB) |
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| ISSN: | 1983-4632, 1519-0501, 1983-4632 |
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| Abstract | ABSTRACT Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR. |
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| AbstractList | ABSTRACT Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR. Objective: To assess the effects of cobalt chloride (CoCl2) as a hypoxia mimicking agent on human umbilical cord mesenchymal stem cells (hUCMSCs) expression of HIF-1α and mTOR for use in regenerative dentistry. Material and Methods: Human umbilical cord mesenchymal stem cells were isolated and then cultured. The characteristics of stemness were screened and confirmed by flow cytometry. The experiment was conducted on hypoxia (H) and normoxia (N) groups. Each group was divided and incubated into 24-, 48-, and 72-hours observations. Hypoxic treatment was performed using 100 µM CoCl2 on 5th passage cells in a conventional incubator (37°C; 5% CO2). Then, immunofluorescence of HIF-1α and mTOR was done. Data was analyzed statistically using One-way ANOVA and Tukey’s HSD. Results: Significant differences were found between normoxic and hypoxic groups on HIF-1α (p=0.015) and mTOR (p=0.000) expressions. The highest HIF-1α expression was found at 48 hours in the hypoxia group, while for mTOR at 24 hours in the hypoxia group. Conclusion: Hypoxia using cobalt chloride was able to increase human umbilical cord mesenchymal stem cells expression of HIF-1α and mTOR. |
| Author | Rusli, Marvin Kuntjoro, Mefina Dinaryanti, Aristika Prasetyo, Eric Priyo Agustono, Bambang Tjendronegoro, Evelyn Hendrianto, Eryk Hendrijantini, Nike Sitalaksmi, Ratri Maya Hong, Guang |
| AuthorAffiliation | Universitas Airlangga Tohoku University University of California |
| AuthorAffiliation_xml | – name: Universitas Airlangga – name: University of California – name: Tohoku University |
| Author_xml | – sequence: 1 givenname: Mefina surname: Kuntjoro fullname: Kuntjoro, Mefina organization: Universitas Airlangga – sequence: 2 givenname: Nike surname: Hendrijantini fullname: Hendrijantini, Nike organization: Universitas Airlangga – sequence: 3 givenname: Eric Priyo surname: Prasetyo fullname: Prasetyo, Eric Priyo organization: Universitas Airlangga – sequence: 4 givenname: Bambang surname: Agustono fullname: Agustono, Bambang organization: Universitas Airlangga – sequence: 5 givenname: Ratri Maya surname: Sitalaksmi fullname: Sitalaksmi, Ratri Maya organization: Universitas Airlangga – sequence: 6 givenname: Eryk surname: Hendrianto fullname: Hendrianto, Eryk organization: Universitas Airlangga – sequence: 7 givenname: Aristika surname: Dinaryanti fullname: Dinaryanti, Aristika organization: Universitas Airlangga – sequence: 8 givenname: Marvin surname: Rusli fullname: Rusli, Marvin organization: Universitas Airlangga – sequence: 9 givenname: Evelyn surname: Tjendronegoro fullname: Tjendronegoro, Evelyn organization: University of California – sequence: 10 givenname: Guang surname: Hong fullname: Hong, Guang organization: Tohoku University |
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| Keywords | Regeneration Hypoxia Umbilical Cord Mesenchymal Stem Cells Stem Cell Research |
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| Title | Cobalt Chloride as a Hypoxia Mimicking Agent Induced HIF-1α and mTOR Expressions of Human Umbilical Cord Mesenchymal Stem Cells |
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