Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM , bla CTX-M , and tetA antibiotic-resistance genes

This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla , tetA, and bla ). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias garie...

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Published in:Scientific reports Vol. 10; no. 1; pp. 15961 - 12
Main Authors: Algammal, Abdelazeem M, Mabrok, Mahmoud, Sivaramasamy, Elayaraja, Youssef, Fatma M, Atwa, Mona H, El-Kholy, Ali W, Hetta, Helal F, Hozzein, Wael N
Format: Journal Article
Language:English
Published: England Nature Portfolio 01.12.2020
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Drug Resistance, Multiple - genetics
Drug Resistance, Multiple, Bacterial - genetics
Egypt
Fishes - genetics
Fishes - microbiology
Genes, Bacterial - drug effects
Polymerase Chain Reaction
Pseudomonas aeruginosa - genetics
Pseudomonas Infections - genetics
Virulence - genetics
Virulence Factors - genetics
Egypt
ISSN:2045-2322
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Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla , tetA, and bla ). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias gariepinus) were collected randomly from private fish farms in Ismailia Governorate, Egypt. The collected specimens were examined bacteriologically. P. aeruginosa was isolated from 90 examined fish (31.57%), and the liver was the most prominent infected organ. The antibiogram of the isolated strains was determined using a disc diffusion method, where the tested strains exhibited multi-drug resistance (MDR) to amoxicillin, cefotaxime, tetracycline, and gentamicin. The PCR results revealed that all the examined strains harbored (oprL and toxA) virulence genes, while only 22.2% were positive for the phzM gene. On the contrary, none of the tested strains were positive for the exoS gene. Concerning the distribution of the antibiotic resistance genes, the examined strains harbored bla , bla , and tetA genes with a total prevalence of 83.3%, 77.7%, and 75.6%, respectively. Experimentally infected fish with P. aeruginosa displayed high mortalities in direct proportion to the encoded virulence genes and showed similar signs of septicemia found in the naturally infected one. In conclusion, P. aeruginosa is a major pathogen of O. niloticus and C. gariepinus. oprL and toxA genes are the most predominant virulence genes associated with P. aeruginosa infection. The bla , bla , and tetA genes are the main antibiotic-resistance genes that induce resistance patterns to cefotaxime, amoxicillin, and tetracycline, highlighting MDR P. aeruginosa strains of potential public health concern.
AbstractList Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla TEM, tetA, and bla CTX-M). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias gariepinus) were collected randomly from private fish farms in Ismailia Governorate, Egypt. The collected specimens were examined bacteriologically. P. aeruginosa was isolated from 90 examined fish (31.57%), and the liver was the most prominent infected organ. The antibiogram of the isolated strains was determined using a disc diffusion method, where the tested strains exhibited multi-drug resistance (MDR) to amoxicillin, cefotaxime, tetracycline, and gentamicin. The PCR results revealed that all the examined strains harbored (oprL and toxA) virulence genes, while only 22.2% were positive for the phzM gene. On the contrary, none of the tested strains were positive for the exoS gene. Concerning the distribution of the antibiotic resistance genes, the examined strains harbored bla TEM, bla CTX-M , and tetA genes with a total prevalence of 83.3%, 77.7%, and 75.6%, respectively. Experimentally infected fish with P. aeruginosa displayed high mortalities in direct proportion to the encoded virulence genes and showed similar signs of septicemia found in the naturally infected one. In conclusion, P. aeruginosa is a major pathogen of O. niloticus and C. gariepinus. oprL and toxA genes are the most predominant virulence genes associated with P. aeruginosa infection. The bla CTX-M , bla TEM, and tetA genes are the main antibiotic-resistance genes that induce resistance patterns to cefotaxime, amoxicillin, and tetracycline, highlighting MDR P. aeruginosa strains of potential public health concern.
This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla , tetA, and bla ). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias gariepinus) were collected randomly from private fish farms in Ismailia Governorate, Egypt. The collected specimens were examined bacteriologically. P. aeruginosa was isolated from 90 examined fish (31.57%), and the liver was the most prominent infected organ. The antibiogram of the isolated strains was determined using a disc diffusion method, where the tested strains exhibited multi-drug resistance (MDR) to amoxicillin, cefotaxime, tetracycline, and gentamicin. The PCR results revealed that all the examined strains harbored (oprL and toxA) virulence genes, while only 22.2% were positive for the phzM gene. On the contrary, none of the tested strains were positive for the exoS gene. Concerning the distribution of the antibiotic resistance genes, the examined strains harbored bla , bla , and tetA genes with a total prevalence of 83.3%, 77.7%, and 75.6%, respectively. Experimentally infected fish with P. aeruginosa displayed high mortalities in direct proportion to the encoded virulence genes and showed similar signs of septicemia found in the naturally infected one. In conclusion, P. aeruginosa is a major pathogen of O. niloticus and C. gariepinus. oprL and toxA genes are the most predominant virulence genes associated with P. aeruginosa infection. The bla , bla , and tetA genes are the main antibiotic-resistance genes that induce resistance patterns to cefotaxime, amoxicillin, and tetracycline, highlighting MDR P. aeruginosa strains of potential public health concern.
Author Hetta, Helal F
El-Kholy, Ali W
Mabrok, Mahmoud
Youssef, Fatma M
Atwa, Mona H
Algammal, Abdelazeem M
Hozzein, Wael N
Sivaramasamy, Elayaraja
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  fullname: Algammal, Abdelazeem M
  email: abdelazeem.algammal@gmail.com
  organization: Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. abdelazeem.algammal@gmail.com
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  organization: Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt
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  orcidid: 0000-0001-8541-7304
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  fullname: Hetta, Helal F
  organization: Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH, 45267-0595, USA
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  fullname: Hozzein, Wael N
  organization: Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62511, Egypt
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Snippet This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS,...
Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL,...
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StartPage 15961
SubjectTerms Animals
Anti-Bacterial Agents - pharmacology
Drug Resistance, Multiple - genetics
Drug Resistance, Multiple, Bacterial - genetics
Egypt
Fishes - genetics
Fishes - microbiology
Genes, Bacterial - drug effects
Polymerase Chain Reaction
Pseudomonas aeruginosa - genetics
Pseudomonas Infections - genetics
Virulence - genetics
Virulence Factors - genetics
Title Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM , bla CTX-M , and tetA antibiotic-resistance genes
URI https://www.ncbi.nlm.nih.gov/pubmed/32994450
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