Expression levels of MIF, NLRP1 and FOXP3 genes along with biomarker levels in patients with active form of non-segmental generalized vitiligo: A study in South Indian population

Vitiligo, the most widespread hypopigmentary syndrome, is considered to be a multifactorial disease in which the active melanocytes are lost. Vitiligo has been studied in a variety of ways, and several genes have been implicated. In this study we focused on investigating biomarker levels of TNF-α, M...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Wārasān Songkhlā Nakharin Ročník 44; číslo 2; s. 353 - 360
Hlavní autoři: Alokananda Chakraborty, Seelamneni Thulasamma, Shravan Kumar Ghali, Priyanka Pallapolu, Kuna Lahari, Towseef Amin Rafeeqi, Gulam Mohammed Husain, Farhath Jabeen, Ghazala Javed, Mohammed Abdul Waheed, Munawwar Husain Kazmi
Médium: Journal Article
Jazyk:angličtina
Vydáno: Prince of Songkla University 01.04.2022
Témata:
foxp3
mda
mif
nlrp1
tas
tnf-α
vitiligo
ISSN:0125-3395
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract Vitiligo, the most widespread hypopigmentary syndrome, is considered to be a multifactorial disease in which the active melanocytes are lost. Vitiligo has been studied in a variety of ways, and several genes have been implicated. In this study we focused on investigating biomarker levels of TNF-α, MDA and TAS by using ELISA, and along with that mRNA expression levels of MIF, NLRP1 and FoxP3 genes were quantified with qRT-PCR. In expression studies, Non-segmental Generalized Vitiligo (NSV) subjects had a substantial (P <.05) fold change in MIF gene expression compared to healthy subjects, with a 0.7 change in expression level. In FoxP3 and NLRP1, however, there was just an 0.3-fold change in expression. However, there was a substantial increase (P <.01) in TNF-α and (P <.0005) in TAS levels, but no difference in Malondialdehyde levels (MDA). The current study is a baseline study suggesting that MIF, with a 0.7-fold change, may play a key role in the pathogenesis of active NSV subjects, while changes in NLRP1 and FoxP3 mRNA expression levels were weaker. We also found a significant increase (P <.01) in TNF-α and (P <.0005) in TAS levels, which may be noted as a key feature in Vitiligo subjects.
AbstractList Vitiligo, the most widespread hypopigmentary syndrome, is considered to be a multifactorial disease in which the active melanocytes are lost. Vitiligo has been studied in a variety of ways, and several genes have been implicated. In this study we focused on investigating biomarker levels of TNF-α, MDA and TAS by using ELISA, and along with that mRNA expression levels of MIF, NLRP1 and FoxP3 genes were quantified with qRT-PCR. In expression studies, Non-segmental Generalized Vitiligo (NSV) subjects had a substantial (P <.05) fold change in MIF gene expression compared to healthy subjects, with a 0.7 change in expression level. In FoxP3 and NLRP1, however, there was just an 0.3-fold change in expression. However, there was a substantial increase (P <.01) in TNF-α and (P <.0005) in TAS levels, but no difference in Malondialdehyde levels (MDA). The current study is a baseline study suggesting that MIF, with a 0.7-fold change, may play a key role in the pathogenesis of active NSV subjects, while changes in NLRP1 and FoxP3 mRNA expression levels were weaker. We also found a significant increase (P <.01) in TNF-α and (P <.0005) in TAS levels, which may be noted as a key feature in Vitiligo subjects.
Author Priyanka Pallapolu
Kuna Lahari
Gulam Mohammed Husain
Mohammed Abdul Waheed
Alokananda Chakraborty
Ghazala Javed
Seelamneni Thulasamma
Munawwar Husain Kazmi
Shravan Kumar Ghali
Farhath Jabeen
Towseef Amin Rafeeqi
Author_xml – sequence: 1
  fullname: Alokananda Chakraborty
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 2
  fullname: Seelamneni Thulasamma
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 3
  fullname: Shravan Kumar Ghali
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 4
  fullname: Priyanka Pallapolu
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 5
  fullname: Kuna Lahari
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 6
  fullname: Towseef Amin Rafeeqi
  organization: Regional Research Institute of Unani Medicine in Srinagar (RRIUM), Srinagar, India
– sequence: 7
  fullname: Gulam Mohammed Husain
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 8
  fullname: Farhath Jabeen
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 9
  fullname: Ghazala Javed
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 10
  fullname: Mohammed Abdul Waheed
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
– sequence: 11
  fullname: Munawwar Husain Kazmi
  organization: National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, 500038 India
BookMark eNo10EtOwzAQBmAvQAIKB2DnA5Bix86LXVW1UKnQiofELnLicXBx7Sh2C-VYnJC0wGqk0T-fZuYMHVlnAaFLSoaU8yS99isfotZvhjGJ42FCjtApoXESMVYkJ-jM-xUhvG_QU_Q9-Ww78F47iw1swXjsFL6fTa_ww_xxSbGwEk8Xr0uGG7DgsTDONvhDhzdcabcW3Tt0_5Pa4lYEDTb434Sog94CVq5b79l-z8hDs-4Dwhy8Thj9BRJvddBGN-4Gj7APG7nbW09u0xszK7XoYdduTI87e46OlTAeLv7qAL1MJ8_ju2i-uJ2NR_NIUpaGiBYqUzKjOeUVSQqaZyquGaOCpTzNY6aSuqpqpVIpgHBZKMZrlWWVkjznheJsgGa_rnRiVbad7o_dlU7o8tBwXVOKLujaQJmnBclzWpP-zRwSVeUKGOdZxQjNMgbsB_qkgic
ContentType Journal Article
DBID DOA
DOI 10.14456/sjst-psu.2022.50
DatabaseName DOAJ Directory of Open Access Journals
DatabaseTitleList
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals (DOAJ)
  url: https://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
EndPage 360
ExternalDocumentID oai_doaj_org_article_8690881c00124e5fb8fe3447b301773e
GroupedDBID 123
29R
2WC
5VS
ABDBF
ACUHS
ADBBV
ALMA_UNASSIGNED_HOLDINGS
BAWUL
BCNDV
DIK
E3Z
EBD
EOJEC
ESX
GROUPED_DOAJ
GX1
HH5
KQ8
M~E
OBODZ
OK1
OVT
RNS
TUS
XSB
~8M
ID FETCH-LOGICAL-d136t-19f7fd71814b059187f2c331a3646823f5cbbcff6dae04d9f34cf77bfd4849f43
IEDL.DBID DOA
ISSN 0125-3395
IngestDate Tue Oct 14 19:09:25 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Issue 2
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-d136t-19f7fd71814b059187f2c331a3646823f5cbbcff6dae04d9f34cf77bfd4849f43
OpenAccessLink https://doaj.org/article/8690881c00124e5fb8fe3447b301773e
PageCount 8
ParticipantIDs doaj_primary_oai_doaj_org_article_8690881c00124e5fb8fe3447b301773e
PublicationCentury 2000
PublicationDate 2022-04-01
PublicationDateYYYYMMDD 2022-04-01
PublicationDate_xml – month: 04
  year: 2022
  text: 2022-04-01
  day: 01
PublicationDecade 2020
PublicationTitle Wārasān Songkhlā Nakharin
PublicationYear 2022
Publisher Prince of Songkla University
Publisher_xml – name: Prince of Songkla University
SSID ssj0041251
Score 1.8362362
Snippet Vitiligo, the most widespread hypopigmentary syndrome, is considered to be a multifactorial disease in which the active melanocytes are lost. Vitiligo has been...
SourceID doaj
SourceType Open Website
StartPage 353
SubjectTerms foxp3
mda
mif
nlrp1
tas
tnf-α
vitiligo
Title Expression levels of MIF, NLRP1 and FOXP3 genes along with biomarker levels in patients with active form of non-segmental generalized vitiligo: A study in South Indian population
URI https://doaj.org/article/8690881c00124e5fb8fe3447b301773e
Volume 44
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: Directory of Open Access Journals (DOAJ)
  issn: 0125-3395
  databaseCode: DOA
  dateStart: 20030101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.doaj.org/
  omitProxy: false
  ssIdentifier: ssj0041251
  providerName: Directory of Open Access Journals
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3Na9swFBcl7LDLaNlGu37wDj1Oi2XJlt1bOhIa2LJQWsgt2JJecAlOiN0w9mf1L6ye7I7eeunV6D2E3uN9yE-_H2OXvub2WcIKjkmhuIqd4b4qinhko0JHKaaxsIFsQs9m2WKRz19RfdFMWAcP3B3ckBiTskwYysvKJVhm6AilrvSeqbV0FH0jnb80U10MVpS2w_BinHAp86T_n6l8uTBsHpqWUwvq-_74Bz24f4XWH9LK5JB96utBGHX7OGIHrv7MnsZ_-_nUGtY01dPABuH3dPIdZr9u5wJ8-w-TP4u5hBXFKijWm3oFdKcK9J6eRm52L5JVDT14atOtKEKIA6pWSa3v_3njVh3If9BHdx__nIV91VbrarW5ghEEGFrSFTj3YFqTX8H2P_3XF3Y_Gd_9vOE9uQK3QqYtFzlqtD4zCVX6EktkGmMjpShkqtIslpiYsjSIqS1cpGyOUhnUukSrMpWjkl_ZwG_QHTMovH2y2GidolCJ9NJobB7lWHgJo-QJu6YDXm47_IwlIVqHD97Oy97Oy7fs_O09lJyyj2T0bvLmjA3a3aM7Zx_Mvq2a3UVwoWefZcuA
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Expression+levels+of+MIF%2C+NLRP1+and+FOXP3+genes+along+with+biomarker+levels+in+patients+with+active+form+of+non-segmental+generalized+vitiligo%3A+A+study+in+South+Indian+population&rft.jtitle=W%C4%81ras%C4%81n+Songkhl%C4%81+Nakharin&rft.au=Alokananda+Chakraborty&rft.au=Seelamneni+Thulasamma&rft.au=Shravan+Kumar+Ghali&rft.au=Priyanka+Pallapolu&rft.date=2022-04-01&rft.pub=Prince+of+Songkla+University&rft.issn=0125-3395&rft.volume=44&rft.issue=2&rft.spage=353&rft.epage=360&rft_id=info:doi/10.14456%2Fsjst-psu.2022.50&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_8690881c00124e5fb8fe3447b301773e
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0125-3395&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0125-3395&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0125-3395&client=summon