Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation

Accurate, comprehensive, and timely detection of drug-resistant tuberculosis (TB) is essential to inform patient treatment and enable public health surveillance. This is crucial for effective control of TB globally. Whole-genome sequencing (WGS) and targeted next-generation sequencing (NGS) approach...

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Bibliographic Details
Published in:	PLoS medicine Vol. 16; no. 4; p. e1002794
Main Authors:	Colman, Rebecca E., Mace, Aurélien, Seifert, Marva, Hetzel, Jonathan, Mshaiel, Haifa, Suresh, Anita, Lemmer, Darrin, Engelthaler, David M., Catanzaro, Donald G., Young, Amanda G., Denkinger, Claudia M., Rodwell, Timothy C.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 30.04.2019 Public Library of Science (PLoS)
Subjects:	Antitubercular agents Bioinformatics Capital costs Clinical isolates Complexity Computational biology Consortia Control Cost-Benefit Analysis Deoxyribonucleic acid Diagnostic reagents DNA DNA sequencing DNA, Bacterial - analysis Drug resistance Drug Resistance, Multiple, Bacterial - genetics Evaluation Genetic aspects Genome-wide association studies Genomes Genomic libraries Genomics Health surveillance High-Throughput Nucleotide Sequencing - economics High-Throughput Nucleotide Sequencing - instrumentation High-Throughput Nucleotide Sequencing - methods High-throughput screening (Biochemical assaying) Humans Infectious diseases Instrumentation Laboratories Maintenance costs Medical research Medicine Microbial drug resistance Mortality Mutation Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Next-generation sequencing Occupational health Optimization Patients Public health Reproducibility of Results Risk factors Sequence Analysis, DNA - economics Sequence Analysis, DNA - instrumentation Sequence Analysis, DNA - methods Single-nucleotide polymorphism Time Factors Tuberculosis Tuberculosis, Multidrug-Resistant - microbiology Visualization Whole genome sequencing Workflow Workflow software California United States > US Switzerland Arizona
ISSN:	1549-1676, 1549-1277, 1549-1676
Online Access:	Get full text
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