A Key Role for Chd1 in Histone H3 Dynamics at the 3′ Ends of Long Genes in Yeast

Chd proteins are ATP-dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initi...

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Vydáno v:PLoS genetics Ročník 8; číslo 7; s. e1002811
Hlavní autoři: Radman-Livaja, Marta, Quan, Tiffani K., Valenzuela, Lourdes, Armstrong, Jennifer A., van Welsem, Tibor, Kim, TaeSoo, Lee, Laura J., Buratowski, Stephen, van Leeuwen, Fred, Rando, Oliver J., Hartzog, Grant A.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Public Library of Science 01.07.2012
Public Library of Science (PLoS)
Témata:
3' Untranslated Regions - genetics
Animals
Biochemistry, Molecular Biology
Biology
Chromatin
Chromatin Assembly and Disassembly - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Gene expression
Gene Expression Regulation
Genetic aspects
Genetics
Genomics
Health aspects
Histones
Histones - genetics
Histones - metabolism
Life Sciences
Microbial genetics
Nucleosomes - genetics
Nucleosomes - metabolism
Physiological aspects
Polytene Chromosomes
Proteins
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors - genetics
Transcription, Genetic
Yeast
Yeast fungi
United States
Netherlands
ISSN:1553-7404, 1553-7390, 1553-7404
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Shrnutí:Chd proteins are ATP-dependent chromatin remodeling enzymes implicated in biological functions from transcriptional elongation to control of pluripotency. Previous studies of the Chd1 subclass of these proteins have implicated them in diverse roles in gene expression including functions during initiation, elongation, and termination. Furthermore, some evidence has suggested a role for Chd1 in replication-independent histone exchange or assembly. Here, we examine roles of Chd1 in replication-independent dynamics of histone H3 in both Drosophila and yeast. We find evidence of a role for Chd1 in H3 dynamics in both organisms. Using genome-wide ChIP-on-chip analysis, we find that Chd1 influences histone turnover at the 5' and 3' ends of genes, accelerating H3 replacement at the 5' ends of genes while protecting the 3' ends of genes from excessive H3 turnover. Although consistent with a direct role for Chd1 in exchange, these results may indicate that Chd1 stabilizes nucleosomes perturbed by transcription. Curiously, we observe a strong effect of gene length on Chd1's effects on H3 turnover. Finally, we show that Chd1 also affects histone modification patterns over genes, likely as a consequence of its effects on histone replacement. Taken together, our results emphasize a role for Chd1 in histone replacement in both budding yeast and Drosophila melanogaster, and surprisingly they show that the major effects of Chd1 on turnover occur at the 3' ends of genes.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: GAH OJR TKQ LV JAA FvL. Performed the experiments: LV TKQ MR-L TK LJL SB TvW. Analyzed the data: GAH JAA LV MR-L OJR TKQ FvL. Contributed reagents/materials/analysis tools: JAA OJR GAH FvL. Wrote the paper: GAH OJR.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1002811