Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells

Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells an...

Full description

Saved in:

Bibliographic Details
Published in:	PLoS genetics Vol. 15; no. 10; p. e1008355
Main Authors:	Garcin, Edwige B., Gon, Stéphanie, Sullivan, Meghan R., Brunette, Gregory J., Cian, Anne De, Concordet, Jean-Paul, Giovannangeli, Carine, Dirks, Wilhelm G., Eberth, Sonja, Bernstein, Kara A., Prakash, Rohit, Jasin, Maria, Modesti, Mauro
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 04.10.2019 Public Library of Science (PLoS)
Subjects:	Anemia Biochemistry, Molecular Biology Biology and Life Sciences Cancer Cell culture Cell Nucleus - genetics Cells (Biology) Chemoresistance Chemotherapy Chromatids - genetics Cloning CRISPR Deoxyribonucleic acid Developmental biology DNA DNA Damage - genetics DNA repair DNA Repair - genetics DNA-Binding Proteins - genetics Epithelial cells Fanconi syndrome Fanconi's anemia Funding Genes Genome, Human - genetics Genomes Genomics HEK293 Cells Homologous Recombination - genetics Humans Ionizing radiation Life Sciences Mammary gland Medical equipment industry Medical research Medicine and Health Sciences Microorganisms Mitomycin Mitomycin C Molecular biology Museums Mutation Olaparib Phenotypes Physical Sciences Rad51 Recombinase - genetics Recombination Research and Analysis Methods Supervision United States New York Pittsburgh Pennsylvania France United States > US Germany Pennsylvania Polymerase chain reaction Cell disruption Cloning Guide RNA Plasmid construction Genetic loci Complement system Analysis of variance
ISSN:	1553-7404, 1553-7390, 1553-7404
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemotherapeutic resistance deployed by cancers.
AbstractList	Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemotherapeutic resistance deployed by cancers. Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemotherapeutic resistance deployed by cancers. The five classical RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] are important components of the homologous recombination pathway that preserves genomic integrity and protects against cancer. Recently, two RAD51 paralogs have been clearly identified as tumor suppressors and the other three paralogs have been found to be mutated in some tumors. As well, two RAD51 paralogs have been reported to be Fanconi anemia proteins. Thus, there is renewed interest in the RAD51 paralogs from a human health concern and the need for isogenic human cell lines to evaluate tumor-derived mutations and support biochemical approaches aimed at understanding their molecular functions. Here we provide three coherent sets of isogenic mutants, both in transformed and non-transformed human cells. Importantly, using these mutant lines, we report the unanticipated result that RAD51B has a less crucial role in homologous recombination than the other four paralogs, and find that all RAD51 paralogs are critically important for early functions during homologous recombination. Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemo-therapeutic resistance deployed by cancers. Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemotherapeutic resistance deployed by cancers.Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A cells. Underlining their importance for genomic stability, mutant cell lines display variable growth defects, impaired sister chromatid recombination, reduced levels of stable RAD51 nuclear foci, and hyper-sensitivity to mitomycin C and olaparib, with the weakest phenotypes observed in RAD51B-deficient cells. Altogether these observations underscore the contributions of RAD51 paralogs in diverse DNA repair processes, and demonstrate essential differences in different cell types. Finally, this study will provide useful reagents to analyze patient-derived mutations and to investigate mechanisms of chemotherapeutic resistance deployed by cancers.
Audience	Academic
Author	Gon, Stéphanie Garcin, Edwige B. Eberth, Sonja Prakash, Rohit Jasin, Maria Bernstein, Kara A. Giovannangeli, Carine Dirks, Wilhelm G. Concordet, Jean-Paul Sullivan, Meghan R. Brunette, Gregory J. Cian, Anne De Modesti, Mauro
AuthorAffiliation	1 Cancer Research Center of Marseille; CNRS; Inserm; Institut Paoli-Calmettes; Aix-Marseille Université, Marseille, France 4 Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ-German, Collection of Microorganisms and Cell Cultures, Braunschweig, Germany Columbia University, UNITED STATES 2 Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine and UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania, United States of America 3 Museum National d'Histoire Naturelle, Inserm U1154, CNRS UMR 7196, Sorbonne Universités, Paris, France 5 Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America
AuthorAffiliation_xml	– name: 5 Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America – name: Columbia University, UNITED STATES – name: 1 Cancer Research Center of Marseille; CNRS; Inserm; Institut Paoli-Calmettes; Aix-Marseille Université, Marseille, France – name: 3 Museum National d'Histoire Naturelle, Inserm U1154, CNRS UMR 7196, Sorbonne Universités, Paris, France – name: 4 Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ-German, Collection of Microorganisms and Cell Cultures, Braunschweig, Germany – name: 2 Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine and UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania, United States of America
Author_xml	– sequence: 1 givenname: Edwige B. orcidid: 0000-0002-0533-6940 surname: Garcin fullname: Garcin, Edwige B. – sequence: 2 givenname: Stéphanie surname: Gon fullname: Gon, Stéphanie – sequence: 3 givenname: Meghan R. surname: Sullivan fullname: Sullivan, Meghan R. – sequence: 4 givenname: Gregory J. orcidid: 0000-0002-3829-9031 surname: Brunette fullname: Brunette, Gregory J. – sequence: 5 givenname: Anne De orcidid: 0000-0002-1737-2543 surname: Cian fullname: Cian, Anne De – sequence: 6 givenname: Jean-Paul surname: Concordet fullname: Concordet, Jean-Paul – sequence: 7 givenname: Carine surname: Giovannangeli fullname: Giovannangeli, Carine – sequence: 8 givenname: Wilhelm G. surname: Dirks fullname: Dirks, Wilhelm G. – sequence: 9 givenname: Sonja orcidid: 0000-0002-5796-2089 surname: Eberth fullname: Eberth, Sonja – sequence: 10 givenname: Kara A. orcidid: 0000-0003-2247-6459 surname: Bernstein fullname: Bernstein, Kara A. – sequence: 11 givenname: Rohit orcidid: 0000-0001-5981-8980 surname: Prakash fullname: Prakash, Rohit – sequence: 12 givenname: Maria orcidid: 0000-0002-7976-2379 surname: Jasin fullname: Jasin, Maria – sequence: 13 givenname: Mauro surname: Modesti fullname: Modesti, Mauro
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31584931$$D View this record in MEDLINE/PubMed https://hal.science/hal-02383639$$DView record in HAL
BookMark	eNqVk11v0zAUhiM0xD7gHyCIhITYRUvsJHayC6Sqg7VSYah83BrHOW5dJXZnJxP8exyaTU01IVAuEh8_5xy_r3NOgyNtNATBcxSNUUzR241prebVeLsCPUZRlMVp-ig4QWkaj2gSJUd738fBqXObKIrTLKdPguMYpVmSx-gk-HGppAQLulG8Cpdw0yoLtV-6UBobNmsIl5PLFIWfueWVWblQ6fAKtKn9Bmy5siHXZfiRK92A5lpAB8zamutwClXlngaPJa8cPOvfZ8G3D--_TmejxfXVfDpZjESGSTNCkpSYYk4JyqVMqCBZhkBwkoPIZVHkkBdlQXmEMMYJxSkuvWYoOU5Lv0Xjs-Dlru62Mo715jiGY-SFJhhnnpjviNLwDdtaVXP7ixmu2J-AsSvGbaNEBQxKmRJcQkoimuSCFJyWBS5inBOaUJT4Wu_6bm1RQym8Yd6eQdHhjlZrtjK3jNA89cf3Bc53BdYHabPJgnWxCMdZTOL8Fnn2Td_MmpsWXMNq5YQ3l2swbacxQomXSDqNrw7Qh53oqRX3YpWWxp9RdEXZxCsmCY1oR40foPxTQq2E_xml8vFBwvkgwTMN_GxWvHWOzb8s_4P99O_s9fch-3qPXQOvmrUzVdsoo90QfLF_g_d3cDcaHkh2gLDGOQvyHkER6ybwzlrWTSDrJ9CnXRykCdXwrr13T1V_T_4NfZczKw
CitedBy_id	crossref_primary_10_1016_j_cell_2024_08_039 crossref_primary_10_1038_s41586_023_06219_w crossref_primary_10_1016_j_gde_2021_06_010 crossref_primary_10_1038_s41586_023_06179_1 crossref_primary_10_1016_j_dnarep_2021_103227 crossref_primary_10_1016_j_tig_2021_02_008 crossref_primary_10_1093_nar_gkad856 crossref_primary_10_1038_s41388_024_03264_1 crossref_primary_10_7717_peerj_19179 crossref_primary_10_1016_j_dnarep_2024_103644 crossref_primary_10_1038_s41419_021_04405_0 crossref_primary_10_1038_s41467_020_17324_z crossref_primary_10_1158_0008_5472_CAN_22_2319 crossref_primary_10_1146_annurev_genet_021920_092410 crossref_primary_10_1038_s41523_021_00339_0 crossref_primary_10_1038_s41467_025_61283_2 crossref_primary_10_1038_s41418_022_01021_z crossref_primary_10_1038_s41598_024_53047_7 crossref_primary_10_1093_nar_gkae770 crossref_primary_10_1016_j_dnarep_2021_103114 crossref_primary_10_1016_j_pharmthera_2020_107492 crossref_primary_10_1371_journal_pgen_1010639 crossref_primary_10_3389_fonc_2023_1162644 crossref_primary_10_1016_j_dnarep_2023_103613 crossref_primary_10_1038_s41467_021_24205_6 crossref_primary_10_1016_j_semcdb_2020_07_004 crossref_primary_10_1186_s12935_023_03071_w crossref_primary_10_1016_j_dnarep_2023_103563 crossref_primary_10_1158_0008_5472_CAN_21_1843 crossref_primary_10_1038_s41467_024_51595_0 crossref_primary_10_1073_pnas_2202727119 crossref_primary_10_1002_humu_24319 crossref_primary_10_3892_mmr_2022_12774 crossref_primary_10_1097_CM9_0000000000003471 crossref_primary_10_1371_journal_pgen_1010495
Cites_doi	10.1083/jcb.200811079 10.1155/2010/904767 10.1074/jbc.M211038200 10.1136/jmedgenet-2016-103847 10.1074/jbc.M108306200 10.1038/35036399 10.1093/emboj/20.19.5513 10.1016/S0300-9084(99)80041-8 10.1093/hmg/dds115 10.1038/nature03404 10.1074/jbc.M109.024646 10.1093/nar/26.5.1179 10.1158/0008-5472.CAN-06-3672 10.1093/nar/gki766 10.1093/carcin/bgq210 10.1073/pnas.0902076106 10.1016/j.dnarep.2013.05.005 10.1093/nar/gkl020 10.1128/MCB.01235-06 10.1186/1476-4598-9-180 10.1016/j.cell.2015.06.015 10.1093/nar/26.13.3084 10.1002/(SICI)1526-968X(200003)26:3<167::AID-GENE1>3.0.CO;2-M 10.1073/pnas.0604232103 10.1128/MCB.21.8.2858-2866.2001 10.1074/jbc.M405212200 10.1093/nar/gkp262 10.1074/jbc.M001473200 10.1074/jbc.R300027200 10.1016/S0921-8777(99)00010-5 10.1002/1873-3468.12556 10.1073/pnas.91.14.6319 10.1186/1471-2407-13-484 10.1101/gad.12.24.3831 10.1016/j.dnarep.2004.08.010 10.2741/A304 10.1158/0008-5472.CAN-05-2803 10.1074/jbc.273.34.21482 10.1016/j.molcel.2016.10.038 10.1093/nar/30.4.1009 10.1126/science.aac7557 10.1038/ng.570 10.1128/MCB.18.11.6423 10.1038/ng.2417 10.1093/nar/gkh578 10.1038/43932 10.1016/j.celrep.2018.11.085 10.1007/978-1-62703-128-8_3 10.1186/bcr2619 10.1074/jbc.M201402200 10.1074/jbc.M002075200 10.1016/j.cell.2015.11.015 10.1038/ng.893 10.1128/MCB.20.17.6476-6482.2000 10.1158/0008-5472.CAN-04-2079 10.1128/MCB.01406-08 10.1002/ijc.24999 10.1093/emboj/19.24.6675 10.1006/geno.1997.4636 10.1158/2159-8290.CD-17-0419 10.1101/gad.935501 10.1101/gad.13.20.2633 10.1093/nar/gkl366 10.1006/geno.1998.5226 10.1038/ng.2224 10.1016/j.dnarep.2012.12.007 10.1038/nature10522 10.1371/journal.pone.0125622 10.1128/MCB.19.12.8686 10.1038/nrc3181 10.1006/geno.1997.5062 10.1038/nature03443 10.1073/pnas.111005698 10.1038/nprot.2013.143 10.1002/0471142727.mb2804s106 10.1038/ncomms8834 10.1186/s12859-015-0816-5 10.1073/pnas.92.15.6925 10.1074/jbc.M111.271080 10.1093/femsyr/fow073 10.1038/sj.emboj.7600087 10.1101/gad.11.9.1111 10.1101/gad.947001 10.1126/science.aac7041 10.1128/MCB.15.9.4843 10.1016/j.ajhg.2012.02.027 10.1016/j.molcel.2016.10.020 10.1242/jcs.114595 10.1073/pnas.92.14.6354 10.1016/j.cell.2018.08.056 10.1038/nbt.1562 10.1038/sj.emboj.7600914 10.1016/j.dnarep.2006.10.024 10.1093/nar/30.10.2172 10.1016/j.dnarep.2019.02.008 10.1016/j.mrfmmm.2010.05.001 10.4161/cc.4.5.1689 10.1101/cshperspect.a016600 10.1016/S1097-2765(00)80078-7 10.1038/s41467-017-00634-0 10.1007/s10549-010-1095-5 10.1128/MCB.00465-12 10.1074/jbc.M111.311241
ContentType	Journal Article
Copyright	COPYRIGHT 2019 Public Library of Science 2019 Garcin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Attribution 2019 Garcin et al 2019 Garcin et al
Copyright_xml	– notice: COPYRIGHT 2019 Public Library of Science – notice: 2019 Garcin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Attribution – notice: 2019 Garcin et al 2019 Garcin et al
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISN ISR 3V. 7QP 7QR 7SS 7TK 7TM 7TO 7X7 7XB 88E 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7P P64 PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS RC3 7X8 1XC VOOES 5PM DOA
DOI	10.1371/journal.pgen.1008355
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Canada Gale In Context: Science ProQuest Central (Corporate) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Entomology Abstracts (Full archive) Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Journals ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Local Electronic Collection Information Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences ProQuest Health & Medical Collection Medical Database Biological Science Database Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student Oncogenes and Growth Factors Abstracts Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials Nucleic Acids Abstracts ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection Genetics Abstracts Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection AIDS and Cancer Research Abstracts Chemoreception Abstracts ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Entomology Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE Publicly Available Content Database MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database (ProQuest) url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
DocumentTitleAlternate	Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
EISSN	1553-7404
ExternalDocumentID	2314934228 oai_doaj_org_article_edf562de560749c6ba7db2b329674714 PMC6795472 oai:HAL:hal-02383639v1 A607647078 31584931 10_1371_journal_pgen_1008355
Genre	Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural
GeographicLocations	United States New York Pittsburgh Pennsylvania France United States--US Germany Pennsylvania
GeographicLocations_xml	– name: United States – name: New York – name: Pennsylvania – name: Germany – name: Pittsburgh Pennsylvania – name: France – name: United States--US
GrantInformation_xml	– fundername: NIGMS NIH HHS grantid: F32 GM110978 – fundername: NIGMS NIH HHS grantid: R35 GM118175 – fundername: NCI NIH HHS grantid: R35 CA253174 – fundername: NIEHS NIH HHS grantid: R01 ES024872 – fundername: NCI NIH HHS grantid: P30 CA008748 – fundername: NIEHS NIH HHS grantid: F31 ES027321 – fundername: NCI NIH HHS grantid: R01 CA185660
GroupedDBID	--- 123 29O 2WC 53G 5VS 7X7 88E 8FE 8FH 8FI 8FJ AAFWJ AAUCC AAWOE AAYXX ABDBF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV AEAQA AENEX AFFHD AFKRA AFPKN AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS B0M BAIFH BAWUL BBNVY BBTPI BCNDV BENPR BHPHI BPHCQ BVXVI BWKFM CCPQU CITATION CS3 DIK DU5 E3Z EAP EAS EBD EBS EJD EMK EMOBN ESX F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HMCUK HYE IAO IGS IHR IHW INH INR IOV ISN ISR ITC KQ8 LK8 M1P M48 M7P O5R O5S OK1 OVT P2P PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PV9 QF4 QN7 RNS RPM RZL SV3 TR2 TUS UKHRP WOW XSB ~8M ADRAZ ALIPV C1A CGR CUY CVF ECM EIF H13 IPNFZ NPM RIG WOQ 3V. 7QP 7QR 7SS 7TK 7TM 7TO 7XB 8FD 8FK AZQEC DWQXO FR3 GNUQQ H94 K9. P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 1XC VOOES 5PM AAPBV ABPTK ACDSR BBAFP M~E N95 UMP
ID	FETCH-LOGICAL-c826t-1f6d272a7619ff47c6881eca69ec9fbb9e9bdb7a0122247252d100eda25de9b73
IEDL.DBID	FPL
ISICitedReferencesCount	44
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000503176900011&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1553-7404 1553-7390
IngestDate	Sun Nov 06 00:11:35 EDT 2022 Fri Oct 03 12:51:51 EDT 2025 Tue Nov 04 01:32:29 EST 2025 Tue Oct 14 20:50:44 EDT 2025 Fri Sep 05 07:24:11 EDT 2025 Sat Nov 29 14:33:15 EST 2025 Tue Nov 11 10:34:26 EST 2025 Tue Nov 04 18:06:22 EST 2025 Thu Nov 13 15:23:27 EST 2025 Thu Nov 13 14:25:55 EST 2025 Thu Nov 13 15:17:29 EST 2025 Thu May 22 21:08:31 EDT 2025 Thu Apr 03 06:54:45 EDT 2025 Sat Nov 29 04:23:52 EST 2025 Tue Nov 18 21:21:49 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	10
Keywords	Polymerase chain reaction Cell disruption Cloning Guide RNA Plasmid construction Genetic loci Complement system Analysis of variance
Language	English
License	Attribution: http://creativecommons.org/licenses/by This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c826t-1f6d272a7619ff47c6881eca69ec9fbb9e9bdb7a0122247252d100eda25de9b73
Notes	new_version ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 The authors declare no competing interests.
ORCID	0000-0002-3829-9031 0000-0002-1737-2543 0000-0002-7976-2379 0000-0003-2247-6459 0000-0002-0533-6940 0000-0001-5981-8980 0000-0002-5796-2089 0000-0002-4964-331X 0000-0002-1513-5018 0000-0001-8924-4316 0000-0002-7715-8864
OpenAccessLink	http://dx.doi.org/10.1371/journal.pgen.1008355
PMID	31584931
PQID	2314934228
PQPubID	1436339
ParticipantIDs	plos_journals_2314934228 doaj_primary_oai_doaj_org_article_edf562de560749c6ba7db2b329674714 pubmedcentral_primary_oai_pubmedcentral_nih_gov_6795472 hal_primary_oai_HAL_hal_02383639v1 proquest_miscellaneous_2301442268 proquest_journals_2314934228 gale_infotracmisc_A607647078 gale_infotracacademiconefile_A607647078 gale_incontextgauss_ISR_A607647078 gale_incontextgauss_ISN_A607647078 gale_incontextgauss_IOV_A607647078 gale_healthsolutions_A607647078 pubmed_primary_31584931 crossref_primary_10_1371_journal_pgen_1008355 crossref_citationtrail_10_1371_journal_pgen_1008355
PublicationCentury	2000
PublicationDate	20191004
PublicationDateYYYYMMDD	2019-10-04
PublicationDate_xml	– month: 10 year: 2019 text: 20191004 day: 4
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: San Francisco – name: San Francisco, CA USA
PublicationTitle	PLoS genetics
PublicationTitleAlternate	PLoS Genet
PublicationYear	2019
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	DL Pittman (pgen.1008355.ref030) 2000; 26 J Essers (pgen.1008355.ref106) 2002 MR Taylor (pgen.1008355.ref012) 2016; 64 MK Dosanjh (pgen.1008355.ref016) 1998; 26 CC Uphoff (pgen.1008355.ref109) 2010; 2010 C Loveday (pgen.1008355.ref072) 2012; 44 RA Baldock (pgen.1008355.ref102) 2019; 76 CS Griffin (pgen.1008355.ref045) 2000; 2 YC Lio (pgen.1008355.ref007) 2003; 278 S Sigurdsson (pgen.1008355.ref009) 2001; 15 DL Pittman (pgen.1008355.ref017) 1998; 49 C Wiese (pgen.1008355.ref024) 2006; 34 N Liu (pgen.1008355.ref042) 1998; 1 JP Braybrooke (pgen.1008355.ref019) 2000; 275 O Kondrashova (pgen.1008355.ref103) 2017; 7 P. Sung (pgen.1008355.ref010) 1997; 11 RD Johnson (pgen.1008355.ref086) 1995; 15 G Nagaraju (pgen.1008355.ref065) 2006; 26 J Liu (pgen.1008355.ref101) 2011; 479 BC Godthelp (pgen.1008355.ref039) 2002; 30 M Takata (pgen.1008355.ref044) 2000; 20 JS Albala (pgen.1008355.ref014) 2002; 46 MR Akbari (pgen.1008355.ref068) 2010; 12 N Puget (pgen.1008355.ref067) 2005; 4 O Date (pgen.1008355.ref055) 2006; 66 G Nagaraju (pgen.1008355.ref066) 2009; 29 T Wang (pgen.1008355.ref093) 2017 P Sung (pgen.1008355.ref050) 2003; 278 JY Park (pgen.1008355.ref078) 2016; 53 B Deans (pgen.1008355.ref056) 2003; 63 B Deans (pgen.1008355.ref029) 2000; 19 S Badie (pgen.1008355.ref053) 2009; 185 AM Kolinjivadi (pgen.1008355.ref003) 2017; 591 R Roy (pgen.1008355.ref059) 2012 Y Zheng (pgen.1008355.ref077) 2010; 124 T Hart (pgen.1008355.ref091) 2015; 163 J. Thacker (pgen.1008355.ref004) 1999; 81 T Yoshihara (pgen.1008355.ref051) 2004; 23 Z Lin (pgen.1008355.ref001) 2006; 103 YC Lio (pgen.1008355.ref061) 2004; 279 F Esashi (pgen.1008355.ref084) 2005; 434 A Lapytsko (pgen.1008355.ref107) 2015; 16 F Vaz (pgen.1008355.ref080) 2010; 42 M Tarsounas (pgen.1008355.ref064) 2005; 4 PG Smiraldo (pgen.1008355.ref048) 2005; 65 J Chun (pgen.1008355.ref057) 2013; 33 JM Hinz (pgen.1008355.ref040) 2006; 34 RD Johnson (pgen.1008355.ref041) 1999; 401 N Orr (pgen.1008355.ref074) 2012; 44 D Schild (pgen.1008355.ref023) 2000; 275 T Wang (pgen.1008355.ref092) 2015; 350 JY Masson (pgen.1008355.ref008) 2001; 98 G Smeenk (pgen.1008355.ref032) 2010; 689 R Amunugama (pgen.1008355.ref005) 2013; 12 N. Liu (pgen.1008355.ref020) 2002; 30 S Saxena (pgen.1008355.ref052) 2018; 25 K Somyajit (pgen.1008355.ref079) 2010; 31 A. Fujimori (pgen.1008355.ref087) 2001; 20 RS Tebbs (pgen.1008355.ref018) 1995; 92 M Takata (pgen.1008355.ref035) 2001; 21 CC Uphoff (pgen.1008355.ref108) 2014 J Martino (pgen.1008355.ref027) 2016; 16 A Rodrigue (pgen.1008355.ref047) 2006; 25 WG Dirks (pgen.1008355.ref111) 2013 SA Compton (pgen.1008355.ref063) 2007; 67 SL Hays (pgen.1008355.ref085) 1995; 92 JY Park (pgen.1008355.ref088) 2016; 53 C Richardson (pgen.1008355.ref105) 1998; 12 CM Abreu (pgen.1008355.ref028) 2018 RB Jensen (pgen.1008355.ref058) 2013; 12 J Adam (pgen.1008355.ref034) 2007; 6 R Cartwright (pgen.1008355.ref015) 1998; 26 FA Ran (pgen.1008355.ref104) 2013; 8 W Feng (pgen.1008355.ref081) 2017; 8 K Somyajit (pgen.1008355.ref062) 2013 JM Buerstedde (pgen.1008355.ref098) 1991 CC Uphoff (pgen.1008355.ref110) 2015; 10 M Katsura (pgen.1008355.ref046) 2009; 37 K Somyajit (pgen.1008355.ref049) 2015; 43 CA French (pgen.1008355.ref038) 2002; 277 VA Blomen (pgen.1008355.ref097) 2015; 350 H Yokoyama (pgen.1008355.ref013) 2004; 32 AJ Pierce (pgen.1008355.ref043) 1999; 13 CE Tambini (pgen.1008355.ref036) 1997; 41 T Liu (pgen.1008355.ref026) 2011; 286 L Golmard (pgen.1008355.ref069) 2013; 13 D Hockemeyer (pgen.1008355.ref083) 2009; 27 E Brunet (pgen.1008355.ref082) 2009; 106 MR Taylor (pgen.1008355.ref011) 2015; 162 R Prakash (pgen.1008355.ref033) 2015; 7 A Osorio (pgen.1008355.ref073) 2012; 21 HE Bryant (pgen.1008355.ref089) 2005; 434 Z Shu (pgen.1008355.ref031) 1999; 19 C Loveday (pgen.1008355.ref071) 2011; 43 T Yasuhara (pgen.1008355.ref096) 2018; 175 KA Miller (pgen.1008355.ref022) 2002; 277 BG Debeb (pgen.1008355.ref090) 2010; 9 X Cui (pgen.1008355.ref054) 1999; 434 A Rodrigue (pgen.1008355.ref060) 2013; 126 DJ Park (pgen.1008355.ref075) 2012; 90 SK Sotiriou (pgen.1008355.ref095) 2016; 64 DK Bishop (pgen.1008355.ref037) 1998; 273 WA Gaines (pgen.1008355.ref006) 2015; 6 JY Masson (pgen.1008355.ref021) 2001; 15 OS Gildemeister (pgen.1008355.ref099) 2009; 284 K Somyajit (pgen.1008355.ref076) 2012; 287 T Rijkers (pgen.1008355.ref094) 1998; 18 WG Dirks (pgen.1008355.ref112) 2010; 126 Y Yonetani (pgen.1008355.ref025) 2005; 33 NP Bhattacharyya (pgen.1008355.ref100) 1994; 91 PR Bianco (pgen.1008355.ref002) 1998; 3 L Golmard (pgen.1008355.ref070) 2017
References_xml	– volume: 185 start-page: 587 year: 2009 ident: pgen.1008355.ref053 article-title: RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation publication-title: J Cell Biol doi: 10.1083/jcb.200811079 – volume: 2010 start-page: 1 year: 2010 ident: pgen.1008355.ref109 article-title: Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in Human and Other Primate Cell Lines. publication-title: J Biomed Biotechnol doi: 10.1155/2010/904767 – volume: 278 start-page: 2469 year: 2003 ident: pgen.1008355.ref007 article-title: Complex formation by the human Rad51B and Rad51C DNA repair proteins and their activities in vitro publication-title: J Biol Chem doi: 10.1074/jbc.M211038200 – volume: 53 start-page: 672 year: 2016 ident: pgen.1008355.ref078 article-title: Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene publication-title: J Med Genet doi: 10.1136/jmedgenet-2016-103847 – volume: 277 start-page: 8406 year: 2002 ident: pgen.1008355.ref022 article-title: RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51 publication-title: J Biol Chem doi: 10.1074/jbc.M108306200 – volume: 2 start-page: 757 year: 2000 ident: pgen.1008355.ref045 article-title: Mammalian recombination-repair genes XRCC2 and XRCC3 promote correct chromosome segregation publication-title: Nat Cell Biol doi: 10.1038/35036399 – volume: 20 start-page: 5513 year: 2001 ident: pgen.1008355.ref087 article-title: Rad52 partially substitutes for the Rad51 paralog XRCC3 in maintaining chromosomal integrity in vertebrate cells publication-title: EMBO J doi: 10.1093/emboj/20.19.5513 – volume: 43 start-page: 9835 year: 2015 ident: pgen.1008355.ref049 article-title: Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart publication-title: Nucleic Acids Res – volume: 81 start-page: 77 year: 1999 ident: pgen.1008355.ref004 article-title: The role of homologous recombination processes in the repair of severe forms of DNA damage in mammalian cells publication-title: Biochimie doi: 10.1016/S0300-9084(99)80041-8 – volume: 21 start-page: 2889 year: 2012 ident: pgen.1008355.ref073 article-title: Predominance of pathogenic missense variants in the RAD51C gene occurring in breast and ovarian cancer families publication-title: Hum Mol Genet doi: 10.1093/hmg/dds115 – volume: 434 start-page: 598 year: 2005 ident: pgen.1008355.ref084 article-title: CDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair publication-title: Nature doi: 10.1038/nature03404 – volume: 284 start-page: 31945 year: 2009 ident: pgen.1008355.ref099 article-title: Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C publication-title: J Biol Chem doi: 10.1074/jbc.M109.024646 – volume: 26 start-page: 1179 year: 1998 ident: pgen.1008355.ref016 article-title: Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes publication-title: Nucleic Acids Res doi: 10.1093/nar/26.5.1179 – volume: 67 start-page: 1513 year: 2007 ident: pgen.1008355.ref063 article-title: Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells publication-title: Cancer Res doi: 10.1158/0008-5472.CAN-06-3672 – volume: 33 start-page: 4544 year: 2005 ident: pgen.1008355.ref025 article-title: Differential and collaborative actions of Rad51 paralog proteins in cellular response to DNA damage publication-title: Nucleic Acids Res doi: 10.1093/nar/gki766 – volume: 31 start-page: 2031 year: 2010 ident: pgen.1008355.ref079 article-title: RAD51C: a novel cancer susceptibility gene is linked to Fanconi anemia and breast cancer publication-title: Carcinogenesis doi: 10.1093/carcin/bgq210 – volume: 106 start-page: 10620 year: 2009 ident: pgen.1008355.ref082 article-title: Chromosomal translocations induced at specified loci in human stem cells publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.0902076106 – volume: 12 start-page: 723 year: 2013 ident: pgen.1008355.ref005 article-title: The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament publication-title: DNA Repair doi: 10.1016/j.dnarep.2013.05.005 – volume: 34 start-page: 1358 year: 2006 ident: pgen.1008355.ref040 article-title: Repression of mutagenesis by Rad51D-mediated homologous recombination publication-title: Nucleic Acids Res doi: 10.1093/nar/gkl020 – volume: 26 start-page: 8075 year: 2006 ident: pgen.1008355.ref065 article-title: Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C publication-title: Mol Cell Biol doi: 10.1128/MCB.01235-06 – volume: 9 start-page: 180 year: 2010 ident: pgen.1008355.ref090 article-title: Characterizing cancer cells with cancer stem cell-like features in 293T human embryonic kidney cells publication-title: Mol Cancer doi: 10.1186/1476-4598-9-180 – year: 2018 ident: pgen.1008355.ref028 article-title: Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination publication-title: Nat Commun – volume: 162 start-page: 271 year: 2015 ident: pgen.1008355.ref011 article-title: Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination publication-title: Cell doi: 10.1016/j.cell.2015.06.015 – volume: 26 start-page: 3084 year: 1998 ident: pgen.1008355.ref015 article-title: The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family publication-title: Nucleic Acids Res doi: 10.1093/nar/26.13.3084 – volume: 26 start-page: 167 year: 2000 ident: pgen.1008355.ref030 article-title: Midgestation lethality in mice deficient for the RecA-related gene, Rad51d/Rad51l3 publication-title: Genesis doi: 10.1002/(SICI)1526-968X(200003)26:3<167::AID-GENE1>3.0.CO;2-M – volume: 63 start-page: 8181 year: 2003 ident: pgen.1008355.ref056 article-title: Homologous Recombination Deficiency Leads to Profound Genetic Instability in Cells Derived from Xrcc2- publication-title: Knockout Mice. Cancer Res – volume: 103 start-page: 10328 year: 2006 ident: pgen.1008355.ref001 article-title: Origins and evolution of the recA/RAD51 gene family: evidence for ancient gene duplication and endosymbiotic gene transfer publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0604232103 – volume: 21 start-page: 2858 year: 2001 ident: pgen.1008355.ref035 article-title: Chromosome instability and defective recombinational repair in knockout mutants of the five Rad51 paralogs publication-title: Mol Cell Biol doi: 10.1128/MCB.21.8.2858-2866.2001 – volume: 279 start-page: 42313 year: 2004 ident: pgen.1008355.ref061 article-title: Human Rad51C deficiency destabilizes XRCC3, impairs recombination, and radiosensitizes S/G2-phase cells publication-title: J Biol Chem doi: 10.1074/jbc.M405212200 – volume: 37 start-page: 3959 year: 2009 ident: pgen.1008355.ref046 article-title: The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction publication-title: Nucleic Acids Res doi: 10.1093/nar/gkp262 – volume: 275 start-page: 16443 year: 2000 ident: pgen.1008355.ref023 article-title: Evidence for simultaneous protein interactions between human Rad51 paralogs publication-title: J Biol Chem doi: 10.1074/jbc.M001473200 – volume: 278 start-page: 42729 year: 2003 ident: pgen.1008355.ref050 article-title: Rad51 recombinase and recombination mediators publication-title: J Biol Chem doi: 10.1074/jbc.R300027200 – volume: 434 start-page: 75 year: 1999 ident: pgen.1008355.ref054 article-title: The XRCC2 and XRCC3 repair genes are required for chromosome stability in mammalian cells publication-title: Mutat Res doi: 10.1016/S0921-8777(99)00010-5 – volume: 591 start-page: 1083 year: 2017 ident: pgen.1008355.ref003 article-title: Moonlighting at replication forks—a new life for homologous recombination proteins BRCA1, BRCA2 and RAD51 publication-title: FEBS Lett doi: 10.1002/1873-3468.12556 – volume: 91 start-page: 6319 year: 1994 ident: pgen.1008355.ref100 article-title: Mutator phenotypes in human colorectal carcinoma cell lines publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.91.14.6319 – volume: 13 start-page: 484 year: 2013 ident: pgen.1008355.ref069 article-title: Germline mutation in the RAD51B gene confers predisposition to breast cancer publication-title: BMC Cancer doi: 10.1186/1471-2407-13-484 – volume: 12 start-page: 3831 year: 1998 ident: pgen.1008355.ref105 article-title: Double-strand break repair by interchromosomal recombination: suppression of chromosomal translocations publication-title: Genes Dev doi: 10.1101/gad.12.24.3831 – volume: 4 start-page: 149 year: 2005 ident: pgen.1008355.ref067 article-title: Molecular analysis of sister chromatid recombination in mammalian cells publication-title: DNA Repair doi: 10.1016/j.dnarep.2004.08.010 – volume: 3 start-page: D570 year: 1998 ident: pgen.1008355.ref002 article-title: DNA strand exchange proteins: a biochemical and physical comparison publication-title: Front Biosci doi: 10.2741/A304 – volume: 66 start-page: 6018 year: 2006 ident: pgen.1008355.ref055 article-title: Haploinsufficiency of RAD51B Causes Centrosome Fragmentation and Aneuploidy in Human Cells publication-title: Cancer Res doi: 10.1158/0008-5472.CAN-05-2803 – volume: 273 start-page: 21482 year: 1998 ident: pgen.1008355.ref037 article-title: Xrcc3 Is Required for Assembly of Rad51 Complexes in Vivo publication-title: J Biol Chem doi: 10.1074/jbc.273.34.21482 – volume: 64 start-page: 1127 year: 2016 ident: pgen.1008355.ref095 article-title: Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks publication-title: Mol Cell doi: 10.1016/j.molcel.2016.10.038 – volume: 30 start-page: 1009 year: 2002 ident: pgen.1008355.ref020 article-title: Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells publication-title: Nucleic Acids Res doi: 10.1093/nar/30.4.1009 – volume: 350 start-page: 1092 year: 2015 ident: pgen.1008355.ref097 article-title: Gene essentiality and synthetic lethality in haploid human cells publication-title: Science doi: 10.1126/science.aac7557 – volume: 42 start-page: 406 year: 2010 ident: pgen.1008355.ref080 article-title: Mutation of the RAD51C gene in a Fanconi anemia-like disorder publication-title: Nat Genet doi: 10.1038/ng.570 – volume: 18 start-page: 6423 year: 1998 ident: pgen.1008355.ref094 article-title: Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation publication-title: Mol Cell Biol doi: 10.1128/MCB.18.11.6423 – volume: 44 start-page: 1182 year: 2012 ident: pgen.1008355.ref074 article-title: Genome-wide association study identifies a common variant in RAD51B associated with male breast cancer risk publication-title: Nat Genet doi: 10.1038/ng.2417 – volume: 32 start-page: 2556 year: 2004 ident: pgen.1008355.ref013 article-title: Preferential binding to branched DNA strands and strand-annealing activity of the human Rad51B, Rad51C, Rad51D and Xrcc2 protein complex publication-title: Nucleic Acids Res doi: 10.1093/nar/gkh578 – volume: 401 start-page: 397 year: 1999 ident: pgen.1008355.ref041 article-title: Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination publication-title: Nature doi: 10.1038/43932 – volume: 25 start-page: 3273 year: 2018 ident: pgen.1008355.ref052 article-title: XRCC2 Regulates Replication Fork Progression during dNTP Alterations publication-title: Cell Rep doi: 10.1016/j.celrep.2018.11.085 – start-page: 27 year: 2013 ident: pgen.1008355.ref111 article-title: STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination. publication-title: Methods in Molecular Biology doi: 10.1007/978-1-62703-128-8_3 – volume: 12 start-page: 404 year: 2010 ident: pgen.1008355.ref068 article-title: RAD51C germline mutations in breast and ovarian cancer patients publication-title: Breast Cancer Res doi: 10.1186/bcr2619 – volume: 277 start-page: 19322 year: 2002 ident: pgen.1008355.ref038 article-title: Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability publication-title: J Biol Chem doi: 10.1074/jbc.M201402200 – volume: 275 start-page: 29100 year: 2000 ident: pgen.1008355.ref019 article-title: The RAD51 family member, RAD51L3, is a DNA-stimulated ATPase that forms a complex with XRCC2 publication-title: J Biol Chem doi: 10.1074/jbc.M002075200 – volume: 163 start-page: 1515 year: 2015 ident: pgen.1008355.ref091 article-title: High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities publication-title: Cell doi: 10.1016/j.cell.2015.11.015 – volume: 43 start-page: 879 year: 2011 ident: pgen.1008355.ref071 article-title: Germline mutations in RAD51D confer susceptibility to ovarian cancer publication-title: Nat Genet doi: 10.1038/ng.893 – year: 2002 ident: pgen.1008355.ref106 article-title: Analysis of mouse Rad54 expression and its implications for homologous recombination publication-title: DNA Repair (Amst). – volume: 20 start-page: 6476 year: 2000 ident: pgen.1008355.ref044 article-title: The Rad51 paralog Rad51B promotes homologous recombinational repair publication-title: Mol Cell Biol doi: 10.1128/MCB.20.17.6476-6482.2000 – volume: 65 start-page: 2089 year: 2005 ident: pgen.1008355.ref048 article-title: Extensive chromosomal instability in Rad51d-deficient mouse cells publication-title: Cancer Res doi: 10.1158/0008-5472.CAN-04-2079 – volume: 29 start-page: 4283 year: 2009 ident: pgen.1008355.ref066 article-title: XRCC2 and XRCC3 regulate the balance between short- and long-tract gene conversions between sister chromatids publication-title: Mol Cell Biol doi: 10.1128/MCB.01406-08 – volume: 126 start-page: 303 year: 2010 ident: pgen.1008355.ref112 article-title: Cell line cross-contamination initiative: An interactive reference database of STR profiles covering common cancer cell lines publication-title: Int J Cancer doi: 10.1002/ijc.24999 – volume: 19 start-page: 6675 year: 2000 ident: pgen.1008355.ref029 article-title: Xrcc2 is required for genetic stability, embryonic neurogenesis and viability in mice publication-title: EMBO J doi: 10.1093/emboj/19.24.6675 – year: 2017 ident: pgen.1008355.ref070 article-title: Contribution of germline deleterious variants in the RAD51 paralogs to breast and ovarian cancers /631/208/68 /631/67/1347 article publication-title: Eur J Hum Genet – volume: 41 start-page: 84 year: 1997 ident: pgen.1008355.ref036 article-title: The XRCC2 DNA repair gene: identification of a positional candidate publication-title: Genomics doi: 10.1006/geno.1997.4636 – volume: 7 start-page: 984 year: 2017 ident: pgen.1008355.ref103 article-title: Secondary Somatic Mutations Restoring RAD51C and RAD51D Associated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma publication-title: Cancer Discov doi: 10.1158/2159-8290.CD-17-0419 – volume: 15 start-page: 3308 year: 2001 ident: pgen.1008355.ref009 article-title: Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange publication-title: Genes Dev doi: 10.1101/gad.935501 – volume: 13 start-page: 2633 year: 1999 ident: pgen.1008355.ref043 article-title: XRCC3 promotes homology-directed repair of DNA damage in mammalian cells publication-title: Genes Dev doi: 10.1101/gad.13.20.2633 – volume: 53 start-page: 672 year: 2016 ident: pgen.1008355.ref088 article-title: Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene publication-title: J Med Genet doi: 10.1136/jmedgenet-2016-103847 – volume: 34 start-page: 2833 year: 2006 ident: pgen.1008355.ref024 article-title: Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination publication-title: Nucleic Acids Res doi: 10.1093/nar/gkl366 – volume: 49 start-page: 103 year: 1998 ident: pgen.1008355.ref017 article-title: Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene publication-title: Genomics doi: 10.1006/geno.1998.5226 – volume: 44 start-page: 475 year: 2012 ident: pgen.1008355.ref072 article-title: Germline RAD51C mutations confer susceptibility to ovarian cancer publication-title: Nat Genet doi: 10.1038/ng.2224 – volume: 12 start-page: 306 year: 2013 ident: pgen.1008355.ref058 article-title: BRCA2 is epistatic to the RAD51 paralogs in response to DNA damage publication-title: DNA Repair doi: 10.1016/j.dnarep.2012.12.007 – year: 2017 ident: pgen.1008355.ref093 article-title: Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras publication-title: Cell – volume: 479 start-page: 245 year: 2011 ident: pgen.1008355.ref101 article-title: Rad51 paralogues Rad55–Rad57 balance the antirecombinase Srs2 in Rad51 filament formation publication-title: Nature doi: 10.1038/nature10522 – volume: 10 start-page: e0125622 year: 2015 ident: pgen.1008355.ref110 article-title: Prevalence and Characterization of Murine Leukemia Virus Contamination in Human Cell Lines publication-title: PLoS One doi: 10.1371/journal.pone.0125622 – year: 2013 ident: pgen.1008355.ref062 article-title: ATM- and ATR-Mediated Phosphorylation of XRCC3 Regulates DNA Double-Strand Break-Induced Checkpoint Activation and Repair publication-title: Mol Cell Biol – volume: 19 start-page: 8686 year: 1999 ident: pgen.1008355.ref031 article-title: Disruption of muREC2/RAD51L1 in mice results in early embryonic lethality which can Be partially rescued in a p53(-/-) background publication-title: Mol Cell Biol doi: 10.1128/MCB.19.12.8686 – start-page: 68 year: 2012 ident: pgen.1008355.ref059 article-title: BRCA1 and BRCA2: Different roles in a common pathway of genome protection publication-title: Nature Reviews Cancer doi: 10.1038/nrc3181 – volume: 46 start-page: 476 year: 2002 ident: pgen.1008355.ref014 article-title: Identification of a Novel HumanRAD51Homolog,RAD51B publication-title: Genomics doi: 10.1006/geno.1997.5062 – volume: 434 start-page: 913 year: 2005 ident: pgen.1008355.ref089 article-title: Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase publication-title: Nature doi: 10.1038/nature03443 – volume: 98 start-page: 8440 year: 2001 ident: pgen.1008355.ref008 article-title: Complex formation by the human RAD51C and XRCC3 recombination repair proteins publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.111005698 – volume: 8 start-page: 2281 year: 2013 ident: pgen.1008355.ref104 article-title: Genome engineering using the CRISPR-Cas9 system publication-title: Nat Protoc doi: 10.1038/nprot.2013.143 – start-page: 28.4.1 volume-title: Current Protocols in Molecular Biology year: 2014 ident: pgen.1008355.ref108 doi: 10.1002/0471142727.mb2804s106 – volume: 6 start-page: 7834 year: 2015 ident: pgen.1008355.ref006 article-title: Promotion of presynaptic filament assembly by the ensemble of S. cerevisiae Rad51 paralogues with Rad52 publication-title: Nat Commun doi: 10.1038/ncomms8834 – volume: 16 start-page: 392 year: 2015 ident: pgen.1008355.ref107 article-title: FoCo: a simple and robust quantification algorithm of nuclear foci. publication-title: BMC Bioinformatics doi: 10.1186/s12859-015-0816-5 – volume: 92 start-page: 6925 year: 1995 ident: pgen.1008355.ref085 article-title: Firmenich a a, Berg P. Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.92.15.6925 – volume: 286 start-page: 41758 year: 2011 ident: pgen.1008355.ref026 article-title: hSWS1·SWSAP1 is an evolutionarily conserved complex required for efficient homologous recombination repair publication-title: J Biol Chem doi: 10.1074/jbc.M111.271080 – volume: 16 start-page: fow073 year: 2016 ident: pgen.1008355.ref027 article-title: The Shu complex is a conserved regulator of homologous recombination. publication-title: FEMS Yeast Res doi: 10.1093/femsyr/fow073 – volume: 23 start-page: 670 year: 2004 ident: pgen.1008355.ref051 article-title: XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells publication-title: EMBO J doi: 10.1038/sj.emboj.7600087 – volume: 11 start-page: 1111 year: 1997 ident: pgen.1008355.ref010 article-title: Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase publication-title: Genes Dev doi: 10.1101/gad.11.9.1111 – volume: 15 start-page: 3296 year: 2001 ident: pgen.1008355.ref021 article-title: Identification and purification of two distinct complexes containing the five RAD51 paralogs publication-title: Genes Dev doi: 10.1101/gad.947001 – volume: 350 start-page: 1096 year: 2015 ident: pgen.1008355.ref092 article-title: Identification and characterization of essential genes in the human genome publication-title: Science doi: 10.1126/science.aac7041 – volume: 15 start-page: 4843 year: 1995 ident: pgen.1008355.ref086 article-title: Functional differences and interactions among the putative RecA homologs Rad51, Rad55, and Rad57 publication-title: Mol Cell Biol doi: 10.1128/MCB.15.9.4843 – year: 1991 ident: pgen.1008355.ref098 article-title: Increased ratio of targeted to random integration after transfection of chicken B cell lines publication-title: Cell – volume: 90 start-page: 734 year: 2012 ident: pgen.1008355.ref075 article-title: Rare mutations in XRCC2 increase the risk of breast cancer publication-title: Am J Hum Genet doi: 10.1016/j.ajhg.2012.02.027 – volume: 64 start-page: 926 year: 2016 ident: pgen.1008355.ref012 article-title: A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. publication-title: Mol Cell doi: 10.1016/j.molcel.2016.10.020 – volume: 126 start-page: 348 year: 2013 ident: pgen.1008355.ref060 article-title: The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy publication-title: J Cell Sci doi: 10.1242/jcs.114595 – volume: 92 start-page: 6354 year: 1995 ident: pgen.1008355.ref018 article-title: Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.92.14.6354 – volume: 175 start-page: 558 year: 2018 ident: pgen.1008355.ref096 article-title: Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair publication-title: Cell doi: 10.1016/j.cell.2018.08.056 – volume: 27 start-page: 851 year: 2009 ident: pgen.1008355.ref083 article-title: Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases publication-title: Nat Biotechnol doi: 10.1038/nbt.1562 – volume: 25 start-page: 222 year: 2006 ident: pgen.1008355.ref047 article-title: Interplay between human DNA repair proteins at a unique double-strand break in vivo publication-title: EMBO J doi: 10.1038/sj.emboj.7600914 – volume: 6 start-page: 224 year: 2007 ident: pgen.1008355.ref034 article-title: A role for Xrcc2 in the early stages of mouse development publication-title: DNA Repair (Amst). doi: 10.1016/j.dnarep.2006.10.024 – volume: 30 start-page: 2172 year: 2002 ident: pgen.1008355.ref039 article-title: Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability publication-title: Nucleic Acids Res doi: 10.1093/nar/30.10.2172 – volume: 76 start-page: 99 year: 2019 ident: pgen.1008355.ref102 article-title: RAD51D splice variants and cancer-associated mutations reveal XRCC2 interaction to be critical for homologous recombination publication-title: DNA Repair (Amst). doi: 10.1016/j.dnarep.2019.02.008 – volume: 689 start-page: 50 year: 2010 ident: pgen.1008355.ref032 article-title: Rad51C is essential for embryonic development and haploinsufficiency causes increased DNA damage sensitivity and genomic instability publication-title: Mutat Res doi: 10.1016/j.mrfmmm.2010.05.001 – volume: 4 start-page: 672 year: 2005 ident: pgen.1008355.ref064 article-title: Recombination at mammalian telomeres: an alternative mechanism for telomere protection and elongation publication-title: Cell Cycle doi: 10.4161/cc.4.5.1689 – volume: 7 start-page: a016600 year: 2015 ident: pgen.1008355.ref033 article-title: Homologous recombination and human health: the roles of BRCA1, BRCA2, and associated proteins publication-title: Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a016600 – volume: 1 start-page: 783 year: 1998 ident: pgen.1008355.ref042 article-title: XRCC2 and XRCC3, New Human Rad51-Family Members, Promote Chromosome Stability and Protect against DNA Cross-Links and Other Damages publication-title: Mol Cell doi: 10.1016/S1097-2765(00)80078-7 – volume: 8 start-page: 525 year: 2017 ident: pgen.1008355.ref081 article-title: BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination publication-title: Nat Commun doi: 10.1038/s41467-017-00634-0 – volume: 124 start-page: 857 year: 2010 ident: pgen.1008355.ref077 article-title: Screening RAD51C nucleotide alterations in patients with a family history of breast and ovarian cancer publication-title: Breast Cancer Res Treat doi: 10.1007/s10549-010-1095-5 – volume: 33 start-page: 387 year: 2013 ident: pgen.1008355.ref057 article-title: Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway publication-title: Mol Cell Biol doi: 10.1128/MCB.00465-12 – volume: 287 start-page: 3366 year: 2012 ident: pgen.1008355.ref076 article-title: Distinct roles of FANCO/RAD51C protein in DNA damage signaling and repair: implications for Fanconi anemia and breast cancer susceptibility publication-title: J Biol Chem doi: 10.1074/jbc.M111.311241
SSID	ssj0035897
Score	2.4865851
Snippet	Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi...
SourceID	plos doaj pubmedcentral hal proquest gale pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	e1008355
SubjectTerms	Anemia Biochemistry, Molecular Biology Biology and Life Sciences Cancer Cell culture Cell Nucleus - genetics Cells (Biology) Chemoresistance Chemotherapy Chromatids - genetics Cloning CRISPR Deoxyribonucleic acid Developmental biology DNA DNA Damage - genetics DNA repair DNA Repair - genetics DNA-Binding Proteins - genetics Epithelial cells Fanconi syndrome Fanconi's anemia Funding Genes Genome, Human - genetics Genomes Genomics HEK293 Cells Homologous Recombination - genetics Humans Ionizing radiation Life Sciences Mammary gland Medical equipment industry Medical research Medicine and Health Sciences Microorganisms Mitomycin Mitomycin C Molecular biology Museums Mutation Olaparib Phenotypes Physical Sciences Rad51 Recombinase - genetics Recombination Research and Analysis Methods Supervision
SummonAdditionalLinks	– databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9NAEF5BBBIXxLuGAgtC4mQae9de7zG0lCJBqMJDvS37chMpOFGcVOLfM7N2rBhVag9cvZ9jZ2Yf36xnvyHkDRPGlM6JOGW5jXle-Bh4somNy7jzvvBDa0KxCTEeF2dn8nSn1BfmhDXywI3hDrwrYYl2HlZmwaXNjRbOpIalMsd4KiiBAuvZBlPNHMyyoimrkmUsFhDWt4fmmEgOWh-9W4KDMEcAKEjWW5SCdn83Q9-cYoLkYDlf1JeR0H9zKXcWp-N75G7LKumo-Tf3yQ1fPSC3mzqTfx6SX0dtGRQYznM68Zj9G7YFawqclQIHpJPRUZbQU73CzZyazir60VeL39AA69VsRXXl6BeN4hKo0OEREPb_6aGfz-tH5Mfxh--HJ3FbWiG2EE-s46TMXSpSjZsYZcmFzYsi8Vbn0ltZGiO9NM4IjR_eUi7SLHVgKu90mjloEuwxGVSLyu8Ryq00SVLC7Yxxw4z2XA5L7_zQJ0OZFhFhW9sq2-qOY_mLuQof0wTEH42RFHpEtR6JSNzdtWx0N67Av0e3dVhUzQ4XoC-pti-pq_pSRF6i01VzBLUb-2oE-JyjLlJEXgcEKmdUmJpzrjd1rT59_XkN0LfxdUCTHuhtCyoXYDOr2zMTYHmU7eoh93tImCRs_2lTtNiOdU5GnxVeQ9LGgKdeJBHZw26-tW-tgPtzyVAkDn5-2_Uvb37VNeOTMXGv8osNYjBkB44PmCfNSOnegiXAfiWD54reGOq9Zr-lmk2DBHouZAbd8un_8PozcgdYsAwZmnyfDNarjX9ObtmL9axevQjzyl_txHnE priority: 102 providerName: Directory of Open Access Journals – databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELdYAYmX8c0KAwxC4imsiZ04fkJlYwwJSlU-tLcQf2StVJKuaSfx33PnuGFBE0ziNf6lTu_s8-_s8x0hL5hQqjBGBBFLdMCT1AbAk1WgTMyNtakdaOWKTYjRKD0-lmO_4Vb7sMqNTXSG2lQa98j3gIdwyTBh1evFaYBVo_B01ZfQ2CJXMUsCc6F7440lZnHaFFeJYxYIcO791Tkmwj2vqVcLUBNGCgARiTtLk8vg39rprSmGSfYW86q-iIr-GVF5bok6vPm_f-4W2fbklA6b0XSbXLHlHXK9KVf58y75fuCrqYBVmNOJxSBit7tYU6C-FKgknQwP4pCO8yXuCdV0VtJ3tqx-QAMse7MlzUtDP-aYowITfVgEuGMEum_n8_oe-Xr49sv-UeArNAQa3JJVEBaJiUSU415IUXChkzQNrc4TabUslJJWKqNEjud3ERdRHBmQtTV5FBtoEuw-6ZVVaXcI5VqqMCzgdca4Yiq3XA4Ka-zAhgMZpX3CNsrJtE9fjlU05pk7kxPgxjRCylClmVdpnwTtW4smfcc_8G9Q7y0Wk2-7B9XyJPNzObOmANZoLJBFwaVOVC6MihSLZIIuPu-TpzhqsuYma2tCsiHgE47plfrkuUNgAo4SI3xO8nVdZ-8_fbsE6PPoMqBJB_TSg4oKZKZzf_UCJI_ZvzrI3Q4SbI3u9jZFiZ2TztHwQ4bPkPsxoLtnYZ_s4DzZyLfOfo9u-PnN-L-4-VnbjD1j_F9pqzVi0PMHVwEwD5qp1n4FC4FESwb9is4k7Hxmt6WcTV0m9UTIGIblw79_1iNyA2iydCGcfJf0Vsu1fUyu6bPVrF4-cSbnF--XiAU priority: 102 providerName: ProQuest
Title	Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells
URI	https://www.ncbi.nlm.nih.gov/pubmed/31584931 https://www.proquest.com/docview/2314934228 https://www.proquest.com/docview/2301442268 https://hal.science/hal-02383639 https://pubmed.ncbi.nlm.nih.gov/PMC6795472 https://doaj.org/article/edf562de560749c6ba7db2b329674714 http://dx.doi.org/10.1371/journal.pgen.1008355
Volume	15
WOSCitedRecordID	wos000503176900011&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: DOA dateStart: 20050101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: M7P dateStart: 20050701 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: 7X7 dateStart: 20050701 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: BENPR dateStart: 20050701 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database (ProQuest) customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: PIMPY dateStart: 20050701 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1553-7404 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0035897 issn: 1553-7404 databaseCode: FPL dateStart: 20050701 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1bb9MwFLZoBxIv3GGFUQxC4inQxE4cP3aXsklbicpF5SnEl9BKJa2adhL_nnOctCzTJsZLHnI-x87x7Tv28TEhb5lQKjdGeAGLtMej2HrAk5WnTMiNtbHtaeUumxDDYTwey-SvoXhpB58J_0Ot0_cLUCju6QNlCFtkB74doQvXIDndjLwsjKWoj8ddl7Ix_bgo_duxuDVBV8j2YjYvr6Kbl70mL0xDg_v_-wMPyL2acNJ-1UIeklu2eETuVFdQ_n5MfhzWN6RAT5_RkUXHYLdiWFKgsxToIR31D0OfJtkS13lKOi3oR1vMf4EAprLpkmaFoWcZxp3A4B0WAW5rgB7Y2ax8Qr4Ojr4cHHv1rQueBlNj5fl5ZAIRZLi-kedc6CiOfauzSFotc6WklcookeGeXMBFEAYGfsqaLAgNiAR7StrFvLC7hHItle_nkJwxrpjKLJe93Brbs35PBnGHsE1lpLoOSY43Y8xSt88mwDSplJSi7tJadx3ibVMtqpAc_8DvYz1vsRhQ272ASkrr_plakwMTNBYIoOBSRyoTRgWKBTJCs513yCtsJWl1OnU7LKR9wEccQyZ1yBuHwKAaBXrt_MzWZZmefPp2A9Dn4U1AowboXQ3K56AzndXHKUDzGNGrgdxrIGH80M3cJqixC9o57p-m-A75HAMKe-53yC72i41-yxTMAi4Zxo-Dz2_6ytXi11sx5ow-fYWdrxGD1jzQf8A8q7rWthTMB2IsGeQrGp2uUcympJhOXHT0SMgQmuXz60v8gtwF2iudSybfI-3Vcm1fktv6fDUtl13SEmPhnnGX7OwfDZNR163UdN1g00Xv4AQkyclZ8v0PPW97cQ
linkProvider	Public Library of Science
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELbaAoILb-hCoQaBOIUmdhLHB4SWlrJVt0vVFtRbGj_SrrQky2a3qH-K38hMHkuDKuilB67xl9g7Ox5_Y49nCHnFhVKpMcJhPNSOH0bWAZ6sHGUC31gbWVerstiEGAyiw0O5u0B-NndhMKyysYmloTa5xj3yNeAhvuSYsOr9-LuDVaPwdLUpoVGpxbY9-wEuW_FuawP-39eMbX48WO85dVUBRwOVnjpeGhomWIL-e5r6QodR5FmdhNJqmSolrVRGiQTPnJgvWMCM57rWJCww0CQ4fHeRXAMawaIyVHC3sfw8iKpiLkHAHcGlW1_V48JbqzXj7RjUAiMTgPgEraWwrBgwXxcWTzAsc2k8youLqO-fEZznlsTNO_-bMO-S2zX5pt1qttwjCza7T25U5TjPHpCjjbpaDFi9Ed2zGCRd7p4WFKg9BapM97obgUd3kwnueRV0mNFPNsu_QQMs68MJTTJDdxLMwYGJTCwCymMSum5Ho-Ih-XIlP-8RWcryzC4T6mupPC-F1zn3FVeJ9aWbWmNd67mSRR3CG2WIdZ2eHauEjOLyzFGAm1YJKUYVimsV6hBn_ta4Sk_yD_wH1LM5FpOLlw_yyXFc26rYmhRYsbFAhoUvdagSYRRTnMkQtzD8DllFLY2rm7pzExl3AR_6mD6qQ16WCEwwkmEE03EyK4p46_PXS4D2B5cB7bVAb2pQmoPMdFJfLQHJY3azFnKlhQRbqtu9naDEzkmn1-3H-Ay5LQc6f-p1yDLOy0a-Rfx7NsHnm_l2cfOLeTP2jPGNmc1niMGdDXCFAPO4mtrzUXAPnATJoV_RmvStYbZbsuFJmSk-FDIAtXzy92Gtkpu9g51-3N8abD8lt8AlkGW4qr9ClqaTmX1GruvT6bCYPC_NHSVHV20SfgGwo-Xj
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lc9MwENa04TFceEMDhQoGhpOJbdmWdWCY0BCaaQmZFpjeXOvhJjPBDnFSpn-NX8euH6FmOtBLD1ytz5ayWa2-lVa7hLxgXMpEa265LFCWF4TGAp4sLal9TxsTGlvJotgEHw7Dw0MxWiM_67swGFZZ28TCUOtM4R55B3iIJxgmrOokVVjEqNd_O_tuYQUpPGmty2mUKrJrTn-A-5a_GfTgv37puv33n7d3rKrCgKWAVi8sJwm0y90Yffkk8bgKwtAxKg6EUSKRUhghteQxnj-5Hnd9Vzu2bXTs-hqaOIPvrpMrHJOWF2GDo3oVYH5YFnbxfWZxJuzq2h7jTqfSktczUBGMUgAS5DeWxaJ6wGqNWB9jiGZrNs3y82jwn9GcZ5bH_q3_WbC3yc2KlNNuOYvukDWT3iXXyjKdp_fIUa-qIgPWcEr3DQZPF7uqOQXKT4FC0_1uz3foKJ7jXlhOJyn9YNLsGzTAcj-Z0zjV9GOMuTkwwYlBQHF8QrfNdJrfJ18u5ec9IK00S80GoZ4S0nESeJ0xTzIZG0_YidHGNo4t3LBNWK0YkarStmP1kGlUnEVycN9KIUWoTlGlTm1ird6alWlL_oF_hzq3wmLS8eJBNj-OKhsWGZ0AW9YGSDL3hApkzLV0JXNFgFsbXptsocZG5Q3elemMuoAPPEwr1SbPCwQmHklR047jZZ5Hg09fLwA6GF4EtN8AvapASQYyU3F15QQkj1nPGsjNBhJsrGr2NkaJnZHOTncvwmfIeRnQ_BOnTTZwjtbyzaPfMws-X8-985ufrZqxZ4x7TE22RAzueICLBJiH5TRfjYI54DwIBv3yhgFoDLPZkk7GRQb5gAsf1PLR34e1Ra6DJYj2BsPdx-QGeAqiiGL1NklrMV-aJ-SqOllM8vnTwvJRcnTZFuEXzmDuoA
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Differential+Requirements+for+the+RAD51+Paralogs+in+Genome+Repair+and+Maintenance+in+Human+Cells&rft.jtitle=PLoS+genetics&rft.au=Garcin%2C+Edwige+B&rft.au=Gon%2C+St%C3%A9phanie&rft.au=Sullivan%2C+Meghan+R&rft.au=Brunette%2C+Gregory+J&rft.date=2019-10-04&rft.pub=Public+Library+of+Science&rft.issn=1553-7390&rft.volume=15&rft.issue=10&rft_id=info:doi/10.1371%2Fjournal.pgen.1008355&rft.externalDocID=A607647078
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1553-7404&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1553-7404&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1553-7404&client=summon