The Tomato Yellow Leaf Curl Virus Resistance Genes Ty-1 and Ty-3 Are Allelic and Code for DFDGD-Class RNA–Dependent RNA Polymerases

Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus (TYLCV) causes huge losses in tomato production worldwide and is caused by different related begomovirus species. Breeding for TYLCV resistance has been based on the introgression of multiple resistance genes originating...

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Veröffentlicht in:PLoS genetics Jg. 9; H. 3; S. e1003399
Hauptverfasser: Verlaan, Maarten G., Hutton, Samuel F., Ibrahem, Ragy M., Kormelink, Richard, Visser, Richard G. F., Scott, John W., Edwards, Jeremy D., Bai, Yuling
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 01.03.2013
Public Library of Science (PLoS)
Schlagworte:
adenosine kinase
Agriculture
Alleles
Begomovirus - genetics
Begomovirus - pathogenicity
Biology
breeding lines
Chromosomes
Disease Resistance - genetics
Diseases and pests
geminivirus al2
Genes
Genetic aspects
Genetics
Genomes
Genomics
Health aspects
Host-parasite relationships
interfering rnas
l2 proteins
lycopersicon-chilense
mottle virus
nicotiana-attenuata
Physiological aspects
Plant Diseases - genetics
Plant Diseases - virology
Plant immunology
Plant Leaves - genetics
Plant Leaves - virology
Plant viruses
recessive resistance
RNA - genetics
RNA Interference
RNA polymerase
RNA polymerases
RNA-Dependent RNA Polymerase - genetics
RNA-Dependent RNA Polymerase - metabolism
Solanum lycopersicum - genetics
Solanum lycopersicum - virology
Tomatoes
Viral genetics
Virus diseases of plants
Viruses
United States
Netherlands
ISSN:1553-7404, 1553-7390, 1553-7404
Online-Zugang:Volltext
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Zusammenfassung:Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus (TYLCV) causes huge losses in tomato production worldwide and is caused by different related begomovirus species. Breeding for TYLCV resistance has been based on the introgression of multiple resistance genes originating from several wild tomato species. In this study we have fine-mapped the widely used Solanum chilense-derived Ty-1 and Ty-3 genes by screening nearly 12,000 plants for recombination events and generating recombinant inbred lines. Multiple molecular markers were developed and used in combination with disease tests to fine-map the genes to a small genomic region (approximately 70 kb). Using a Tobacco Rattle Virus-Virus Induced Gene Silencing approach, the resistance gene was identified. It is shown that Ty-1 and Ty-3 are allelic and that they code for a RNA-dependent RNA polymerase (RDR) belonging to the RDRγ type, which has an atypical DFDGD motif in the catalytic domain. In contrast to the RDRα type, characterized by a catalytic DLDGD motif, no clear function has yet been described for the RDRγ type, and thus the Ty-1/Ty-3 gene unveils a completely new class of resistance gene. Although speculative, the resistance mechanism of Ty-1/Ty-3 and its specificity towards TYLCV are discussed in light of the function of the related RDRα class in the amplification of the RNAi response in plants and transcriptional silencing of geminiviruses in plants.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Conceived and designed the experiments: MGV SFH RK JWS JDE YB. Performed the experiments: MGV SFH RMI. Analyzed the data: MGV SFH RK JWS YB. Contributed reagents/materials/analysis tools: MGV SFH RK JWS RGFV YB. Wrote the paper: MGV SFH RK RGFV JWS YB.
The authors have declared that no competing interests exist.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1003399