The age and genomic integrity of neurons after cortical stroke in humans

In this study, the authors use measures of carbon-14 in neuronal DNA from human stroke patient cortical tissue samples to show that, unlike previous studies done in rodents, they do not find any evidence of increased neurogenesis after an ischemic injury. In addition, DNA damage assays suggest that...

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Veröffentlicht in:Nature neuroscience Jg. 17; H. 6; S. 801 - 803
Hauptverfasser: Huttner, Hagen B, Bergmann, Olaf, Salehpour, Mehran, Rácz, Attila, Tatarishvili, Jemal, Lindgren, Emma, Csonka, Tamás, Csiba, László, Hortobágyi, Tibor, Méhes, Gábor, Englund, Elisabet, Solnestam, Beata Werne, Zdunek, Sofia, Scharenberg, Christian, Ström, Lena, Ståhl, Patrik, Sigurgeirsson, Benjamin, Dahl, Andreas, Schwab, Stefan, Possnert, Göran, Bernard, Samuel, Kokaia, Zaal, Lindvall, Olle, Lundeberg, Joakim, Frisén, Jonas
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York Nature Publishing Group US 01.06.2014
Nature Publishing Group
Schlagworte:
13/31
13/51
45/23
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631/378/368/2431
Aged
Aged, 80 and over
Analysis
Animal Genetics and Genomics
Basic Medicine
Behavioral Sciences
Biological Techniques
Biomedicine
bomb peak
brief-communication
cell regeneration
Cellular Senescence - physiology
Chromosomes
cortical stroke
DNA
DNA Fragmentation
DNA repair
DNA Repair - physiology
Dynamical Systems
Female
Genetic aspects
Genomes
Hospitals
Humans
Ischemia
Male
Mathematics
Medical and Health Sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Middle Aged
Neocortex - pathology
Neocortex - physiology
Neurobiology
Neurogenesis
Neurons
Neurons - pathology
Neurons - physiology
Neurosciences
Neurovetenskaper
Physiological aspects
Stroke
Stroke (Disease)
Stroke - genetics
Stroke - pathology
Hungary
Sweden
Germany
ISSN:1097-6256, 1546-1726, 1546-1726
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:In this study, the authors use measures of carbon-14 in neuronal DNA from human stroke patient cortical tissue samples to show that, unlike previous studies done in rodents, they do not find any evidence of increased neurogenesis after an ischemic injury. In addition, DNA damage assays suggest that there is no increase in DNA rearrangement after this insult. It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test–derived 14 C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival.
Bibliographie:ObjectType-Article-1
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ISSN:1097-6256
1546-1726
1546-1726
DOI:10.1038/nn.3706