Exploring Microbial Diversity and Taxonomy Using SSU rRNA Hypervariable Tag Sequencing

Massively parallel pyrosequencing of hypervariable regions from small subunit ribosomal RNA (SSU rRNA) genes can sample a microbial community two or three orders of magnitude more deeply per dollar and per hour than capillary sequencing of full-length SSU rRNA. As with full-length rRNA surveys, each...

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Bibliographic Details
Published in:	PLoS genetics Vol. 4; no. 11; p. e1000255
Main Authors:	Huse, Susan M., Dethlefsen, Les, Huber, Julie A., Welch, David Mark, Relman, David A., Sogin, Mitchell L.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 01.11.2008 Public Library of Science (PLoS)
Subjects:	Bacteria - classification Bacteria - genetics Biodiversity Biosphere Biotechnology/Applied Microbiology Biotechnology/Environmental Microbiology Classification - methods Computational Biology/Comparative Sequence Analysis Computational Biology/Metagenomics Computational Biology/Molecular Genetics Deoxyribonucleic acid DNA DNA sequencing Ecology/Community Ecology and Biodiversity Ecology/Environmental Microbiology Evolutionary Biology/Bioinformatics Evolutionary Biology/Microbial Evolution and Genomics Experiments Genes Genetics and Genomics/Microbial Evolution and Genomics Humans Metagenome - genetics Methods Microbial genetics Microbiology Microbiology/Applied Microbiology Microbiology/Environmental Microbiology Microbiology/Medical Microbiology Microbiology/Microbial Evolution and Genomics Nitrogen Nucleotide sequencing Physiological aspects Ribosomal RNA RNA, Ribosomal - genetics Sequence Analysis, DNA Sequence Tagged Sites Taxonomy United States
ISSN:	1553-7404, 1553-7390, 1553-7404
Online Access:	Get full text
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