Structural Basis of Transcriptional Gene Silencing Mediated by Arabidopsis MOM1

Shifts between epigenetic states of transcriptional activity are typically correlated with changes in epigenetic marks. However, exceptions to this rule suggest the existence of additional, as yet uncharacterized, layers of epigenetic regulation. MOM1, a protein of 2,001 amino acids that acts as a t...

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Vydané v:	PLoS genetics Ročník 8; číslo 2; s. e1002484
Hlavní autori:	Nishimura, Taisuke, Molinard, Guillaume, Petty, Tom J., Broger, Larissa, Gabus, Caroline, Halazonetis, Thanos D., Thore, Stéphane, Paszkowski, Jerzy
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 01.02.2012 Public Library of Science (PLoS)
Predmet:	Amino Acid Sequence Arabidopsis - chemistry Arabidopsis - genetics Arabidopsis Proteins - chemistry Arabidopsis Proteins - genetics Arabidopsis thaliana Biology Crystallography, X-Ray Deoxyribonucleic acid Derivatives DNA DNA methylation Efficiency Epigenesis, Genetic - genetics Epigenetics Experiments Gene Expression Regulation, Plant Gene Silencing Genes Genetic aspects Genetic engineering Genetic transcription Molecular Sequence Data Mutagenesis Nuclear Proteins - chemistry Nuclear Proteins - genetics Plants, Genetically Modified Protein Conformation Protein Folding Protein Multimerization Protein Structure, Tertiary - genetics Proteins RNA, Small Interfering - genetics Structure-Activity Relationship Transcription Factors - chemistry Transcription Factors - genetics Transcription, Genetic Japan
ISSN:	1553-7404, 1553-7390, 1553-7404
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Popis
Shrnutí:	Shifts between epigenetic states of transcriptional activity are typically correlated with changes in epigenetic marks. However, exceptions to this rule suggest the existence of additional, as yet uncharacterized, layers of epigenetic regulation. MOM1, a protein of 2,001 amino acids that acts as a transcriptional silencer, represents such an exception. Here we define the 82 amino acid domain called CMM2 (Conserved MOM1 Motif 2) as a minimal MOM1 fragment capable of transcriptional regulation. As determined by X-ray crystallography, this motif folds into an unusual hendecad-based coiled-coil. Structure-based mutagenesis followed by transgenic complementation tests in plants demonstrate that CMM2 and its dimerization are effective for transcriptional suppression at chromosomal loci co-regulated by MOM1 and the siRNA pathway but not at loci controlled by MOM1 in an siRNA-independent fashion. These results reveal a surprising separation of epigenetic activities that enable the single, large MOM1 protein to coordinate cooperating mechanisms of epigenetic regulation.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 a: Current address: PRESTO, Japan Science and Technology Agency, Honcho Kawaguchi, Japan b: Current address: Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan Conceived and designed the experiments: TN TDH ST JP. Performed the experiments: TN GM TJP LB CG TDH ST. Analyzed the data: TN TJP TDH ST JP. Contributed reagents/materials/analysis tools: TN TDH ST JP. Wrote the paper: TN ST JP.
ISSN:	1553-7404 1553-7390 1553-7404
DOI:	10.1371/journal.pgen.1002484