Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy

Single Molecule Localization super-resolution Microscopy (SMLM) has become a powerful tool to study cellular architecture at the nanometer scale. In SMLM, single fluorophore labels are made to repeatedly switch on and off ("blink"), and their exact locations are determined by mathematicall...

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Bibliographic Details
Published in:	PloS one Vol. 11; no. 7; p. e0158884
Main Authors:	Nahidiazar, Leila, Agronskaia, Alexandra V., Broertjes, Jorrit, van den Broek, Bram, Jalink, Kees
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 08.07.2016 Public Library of Science (PLoS)
Subjects:	Biology Biophysics Blinking Buffers Cancer Color Color imagery Diagnostic imaging Dyes Engineering and Technology Fluorescent Dyes - chemistry Image acquisition Image processing Image quality Imaging, Three-Dimensional - methods Labels Lasers Localization Longevity Methods Microscopy Microscopy, Fluorescence, Multiphoton - methods Optimization Physical Sciences Power Research and Analysis Methods Science Stitching Storms Netherlands
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
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