miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in conse...

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Published in:PloS one Vol. 12; no. 3; p. e0174297
Main Authors: Russo, Andrea, Ragusa, Marco, Barbagallo, Cristina, Longo, Antonio, Avitabile, Teresio, Uva, Maurizio G., Bonfiglio, Vincenza, Toro, Mario D., Caltabiano, Rosario, Mariotti, Cesare, Boscia, Francesco, Romano, Mario, Di Pietro, Cinzia, Barbagallo, Davide, Purrello, Michele, Reibaldi, Michele
Format: Journal Article
Language:English
Published: United States Public Library of Science 22.03.2017
Public Library of Science (PLoS)
Subjects:
Analysis
Biology and life sciences
Biomarkers
Biotechnology
Cancer
Comparative studies
Deregulation
Disease
Down-Regulation - genetics
Epiretinal Membrane - genetics
Epiretinal Membrane - metabolism
Epiretinal Membrane - surgery
Female
Gene expression
Genomes
Heart attacks
Humans
Macular degeneration
Male
Medicine and Health Sciences
MicroRNA
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Pathogenesis
Patients
Prospective Studies
Research and analysis methods
Retinal Perforations - genetics
Retinal Perforations - metabolism
Retinal Perforations - surgery
Ribonucleic acid
Risk factors
RNA
Transcriptome - genetics
Vitrectomy - methods
Vitreous Body - metabolism
Vitreous Body - surgery
Vitreous humor
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Summary:The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors. Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p:<0.00004), mir-24 (FC -7.52, p:<0.004) and miR-142-3p (FC -5.32, p:<0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM. The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.
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Competing Interests: The authors have declared that no competing interests exist.
Conceptualization: AR M. Reibaldi.Data curation: CB CDP DB.Formal analysis: M. Ragusa AL.Funding acquisition: M. Romano MP.Investigation: VB MDT.Methodology: MGU CM FB M. Ragusa.Resources: TA.Supervision: MP M. Reibaldi.Validation: RC.Writing – original draft: AR.Writing – review & editing: AR M. Ragusa.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0174297