Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential

Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evalu...

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Published in:Proceedings of the Royal Society. B, Biological sciences Vol. 281; no. 1792
Main Authors: Zouache, Karima, Fontaine, Albin, Vega-Rua, Anubis, Mousson, Laurence, Thiberge, Jean-Michel, Lourenco-De-Oliveira, Ricardo, Caro, Valérie, Lambrechts, Louis, Failloux, Anna-Bella
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Language:English
Published: England 07.10.2014
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ISSN:1471-2954, 1471-2954
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Abstract Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.
AbstractList Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.
Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.
Author Failloux, Anna-Bella
Lambrechts, Louis
Fontaine, Albin
Mousson, Laurence
Caro, Valérie
Vega-Rua, Anubis
Zouache, Karima
Thiberge, Jean-Michel
Lourenco-De-Oliveira, Ricardo
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  organization: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France karima.zouache@pasteur.fr
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  givenname: Albin
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  organization: Department of Genomes and Genetics, Institut Pasteur-Centre National de la Recherche Scientifique URA 3012, Insect-Virus Interactions Group, Paris, France Institut de Recherche Biomédicale des Armées (IRBA), Unité d'Entomologie, Brétigny-sur-Orge, France
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  givenname: Anubis
  surname: Vega-Rua
  fullname: Vega-Rua, Anubis
  organization: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France
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  fullname: Thiberge, Jean-Michel
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  surname: Lourenco-De-Oliveira
  fullname: Lourenco-De-Oliveira, Ricardo
  organization: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil
– sequence: 7
  givenname: Valérie
  surname: Caro
  fullname: Caro, Valérie
  organization: Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France
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  givenname: Louis
  surname: Lambrechts
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  organization: Department of Genomes and Genetics, Institut Pasteur-Centre National de la Recherche Scientifique URA 3012, Insect-Virus Interactions Group, Paris, France
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  givenname: Anna-Bella
  surname: Failloux
  fullname: Failloux, Anna-Bella
  organization: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25122228$$D View this record in MEDLINE/PubMed
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mosquito
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SubjectTerms Aedes - genetics
Aedes - virology
Alphavirus Infections - transmission
Animals
Chikungunya virus - genetics
Genotype
Insect Vectors - genetics
Insect Vectors - virology
Mice
Temperature
Title Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential
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