Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along...

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Bibliographic Details
Published in:	Genome Biology Vol. 23; no. 1; p. 258
Main Authors:	Alonge, Michael, Lebeigle, Ludivine, Kirsche, Melanie, Jenike, Katie, Ou, Shujun, Aganezov, Sergey, Wang, Xingang, Lippman, Zachary B., Schatz, Michael C., Soyk, Sebastian
Format:	Journal Article
Language:	English
Published:	London BioMed Central 15.12.2022 Springer Nature B.V BMC
Subjects:	Animal Genetics and Genomics Assembly scaffolding Automation Bias Bioinformatics Biomedical and Life Sciences Chromosomes Cultivars Evolutionary Biology Experiments Food quality Fruits Gene Editing Genetics Genome Genome editing Genome sequencing Genomes Genomics Genotype Genotypes Human Genetics Life Sciences Microbial Genetics and Genomics Mutation Plant Genetics and Genomics Short Report Solanum lycopersicum - genetics species Tomato tomatoes Assembly scaffolding Genome editing Genome sequencing Tomato
ISSN:	1474-760X, 1474-7596, 1474-760X
Online Access:	Get full text
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