Microbial analysis of in situ biofilm formation in drinking water distribution systems: implications for monitoring and control of drinking water quality

Biofilm formation in drinking water distribution systems (DWDS) is influenced by the source water, the supply infrastructure and the operation of the system. A holistic approach was used to advance knowledge on the development of mixed species biofilms in situ, by using biofilm sampling devices inst...

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Vydané v:	Applied microbiology and biotechnology Ročník 100; číslo 7; s. 3301 - 3311
Hlavní autori:	Douterelo, Isabel, Jackson, M, Solomon, C, Boxall, J
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2016 Springer Springer Nature B.V
Predmet:	Alkalies - chemistry Alkalinity Analytical chemistry Bacteria Basidiobolus Biodiversity biofilm Biofilms Biofilms - growth & development Biomedical and Life Sciences Biotechnology Community structure confocal laser scanning microscopy Confocal microscopy Contamination Control disinfection Drinking water Drinking Water - microbiology Entomophthorales - genetics Entomophthorales - growth & development Entomophthorales - metabolism Environmental Biotechnology Environmental conditions environmental factors Fungi Groundwater Halogenation High density polyethylenes High-Throughput Nucleotide Sequencing Humans Hydraulics infrastructure Laboratories Life Sciences Low flow Methods microbial communities Microbial Consortia - genetics Microbial Genetics and Genomics Microbiology Microorganisms Microscopy monitoring nitrates Nitrates - chemistry pipes Pseudomonas Pseudomonas - genetics Pseudomonas - growth & development Pseudomonas - metabolism Quality control Samples Scanning electron microscopy Scanning microscopy sequence analysis Studies Surface water Water distribution Water distribution systems Water engineering Water Microbiology Water Quality Water quality control Water Supply Water treatment United Kingdom > UK Fungi Bacteria Drinking water distribution systems Biofilms
ISSN:	0175-7598, 1432-0614, 1432-0614
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Shrnutí:	Biofilm formation in drinking water distribution systems (DWDS) is influenced by the source water, the supply infrastructure and the operation of the system. A holistic approach was used to advance knowledge on the development of mixed species biofilms in situ, by using biofilm sampling devices installed in chlorinated networks. Key physico-chemical parameters and conventional microbial indicators for drinking water quality were analysed. Biofilm coverage on pipes was evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The microbial community structure, bacteria and fungi, of water and biofilms was assessed using pyrosequencing. Conventional wisdom leads to an expectation for less microbial diversity in groundwater supplied systems. However, the analysis of bulk water showed higher microbial diversity in groundwater site samples compared with the surface water site. Conversely, higher diversity and richness were detected in biofilms from the surface water site. The average biofilm coverage was similar among sites. Disinfection residual and other key variables were similar between the two sites, other than nitrates, alkalinity and the hydraulic conditions which were extremely low at the groundwater site. Thus, the unexpected result of an exceptionally low diversity with few dominant genera (Pseudomonas and Basidiobolus) in groundwater biofilm samples, despite the more diverse community in the bulk water, is attributed to the low-flow hydraulic conditions. This finding evidences that the local environmental conditions are shaping biofilm formation, composition and amount, and hence managing these is critical for the best operation of DWDS to safeguard water quality.
Bibliografia:	http://dx.doi.org/10.1007/s00253-015-7155-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0175-7598 1432-0614 1432-0614
DOI:	10.1007/s00253-015-7155-3