Interactions among Polycomb Domains Are Guided by Chromosome Architecture

Polycomb group (PcG) proteins bind and regulate hundreds of genes. Previous evidence has suggested that long-range chromatin interactions may contribute to the regulation of PcG target genes. Here, we adapted the Chromosome Conformation Capture on Chip (4C) assay to systematically map chromosomal in...

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Vydáno v:	PLoS genetics Ročník 7; číslo 3; s. e1001343
Hlavní autoři:	Tolhuis, Bas, Blom, Marleen, Kerkhoven, Ron M., Pagie, Ludo, Teunissen, Hans, Nieuwland, Marja, Simonis, Marieke, de Laat, Wouter, van Lohuizen, Maarten, van Steensel, Bas
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 01.03.2011 Public Library of Science (PLoS)
Témata:	Animals Architecture Brain - metabolism Cellular proteins Chromatin Chromatin - metabolism Chromosomes Chromosomes - chemistry Chromosomes - metabolism Chromosomes, Insect - chemistry Chromosomes, Insect - genetics Computational Biology DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Drosophila Drosophila melanogaster - genetics Drosophila melanogaster - metabolism Drosophila Proteins - genetics Drosophila Proteins - metabolism Gene Expression Profiling Gene Expression Regulation Genes, Homeobox - genetics Genetic aspects Genetics Genetics and Genomics/Chromosome Biology Genetics and Genomics/Epigenetics Genetics and Genomics/Nuclear Structure and Function Genomes Genomics Histones - metabolism Insects Larva Physiological aspects Polycomb-Group Proteins Protein Binding Proteins Repressor Proteins - chemistry Repressor Proteins - metabolism Netherlands
ISSN:	1553-7404, 1553-7390, 1553-7404
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Shrnutí:	Polycomb group (PcG) proteins bind and regulate hundreds of genes. Previous evidence has suggested that long-range chromatin interactions may contribute to the regulation of PcG target genes. Here, we adapted the Chromosome Conformation Capture on Chip (4C) assay to systematically map chromosomal interactions in Drosophila melanogaster larval brain tissue. Our results demonstrate that PcG target genes interact extensively with each other in nuclear space. These interactions are highly specific for PcG target genes, because non-target genes with either low or high expression show distinct interactions. Notably, interactions are mostly limited to genes on the same chromosome arm, and we demonstrate that a topological rather than a sequence-based mechanism is responsible for this constraint. Our results demonstrate that many interactions among PcG target genes exist and that these interactions are guided by overall chromosome architecture.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: BT MvL BvS. Performed the experiments: BT MB HT. Analyzed the data: BT. Contributed reagents/materials/analysis tools: RMK LP HT MN MS WdL. Wrote the paper: BT MvL BvS.
ISSN:	1553-7404 1553-7390 1553-7404
DOI:	10.1371/journal.pgen.1001343