mRNA-Seq of Single Prostate Cancer Circulating Tumor Cells Reveals Recapitulation of Gene Expression and Pathways Found in Prostate Cancer

Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expressio...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 7; no. 11; p. e49144
Main Authors:	Cann, Gordon M., Gulzar, Zulfiqar G., Cooper, Samantha, Li, Robin, Luo, Shujun, Tat, Mai, Stuart, Sarah, Schroth, Gary, Srinivas, Sandhya, Ronaghi, Mostafa, Brooks, James D., Talasaz, AmirAli H.
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 07.11.2012 Public Library of Science (PLoS)
Subjects:	Analysis Apoptosis Biology Biomarkers, Tumor - blood Blood Cancer Cancer genetics Cancer metastasis Cell Line, Tumor Engineering Enumeration Gene expression Gene Expression Regulation, Neoplastic Human subjects Humans Kinases Leukocytes Male Medicine Messenger RNA Metabolic Networks and Pathways Metastases Metastasis Neoplasm Metastasis - genetics Neoplasm Metastasis - pathology Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Patients Prognosis Prostate cancer Prostatic Neoplasms - blood Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Ribonucleic acid RNA RNA, Messenger - blood RNA, Messenger - genetics Sequence Alignment Sequence Analysis, RNA Solid tumors Transcription Transcription (Genetics) Tumor cells Tumors Urology United States > US California
ISSN:	1932-6203, 1932-6203
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expression analysis of CTCs, but the technical difficulty of isolating and analyzing individual CTCs has limited progress in this area. To assess the ability of a new generation of MagSweeper to isolate intact CTCs for downstream analysis, we performed mRNA-Seq on single CTCs isolated from the blood of patients with metastatic prostate cancer and on single prostate cancer cell line LNCaP cells spiked into the blood of healthy donors. We found that the MagSweeper effectively isolated CTCs with a capture efficiency that matched the CellSearch platform. However, unlike CellSearch, the MagSweeper facilitates isolation of individual live CTCs without contaminating leukocytes. Importantly, mRNA-Seq analysis showed that the MagSweeper isolation process did not have a discernible impact on the transcriptional profile of single LNCaPs isolated from spiked human blood, suggesting that any perturbations caused by the MagSweeper process on the transcriptional signature of isolated cells are modest. Although the RNA from patient CTCs showed signs of significant degradation, consistent with reports of short half-lives and apoptosis amongst CTCs, transcriptional signatures of prostate tissue and of cancer were readily detectable with single CTC mRNA-Seq. These results demonstrate that the MagSweeper provides access to intact CTCs and that these CTCs can potentially supply clinically relevant information.
AbstractList	Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expression analysis of CTCs, but the technical difficulty of isolating and analyzing individual CTCs has limited progress in this area. To assess the ability of a new generation of MagSweeper to isolate intact CTCs for downstream analysis, we performed mRNA-Seq on single CTCs isolated from the blood of patients with metastatic prostate cancer and on single prostate cancer cell line LNCaP cells spiked into the blood of healthy donors. We found that the MagSweeper effectively isolated CTCs with a capture efficiency that matched the CellSearch platform. However, unlike CellSearch, the MagSweeper facilitates isolation of individual live CTCs without contaminating leukocytes. Importantly, mRNA-Seq analysis showed that the MagSweeper isolation process did not have a discernible impact on the transcriptional profile of single LNCaPs isolated from spiked human blood, suggesting that any perturbations caused by the MagSweeper process on the transcriptional signature of isolated cells are modest. Although the RNA from patient CTCs showed signs of significant degradation, consistent with reports of short half-lives and apoptosis amongst CTCs, transcriptional signatures of prostate tissue and of cancer were readily detectable with single CTC mRNA-Seq. These results demonstrate that the MagSweeper provides access to intact CTCs and that these CTCs can potentially supply clinically relevant information. Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expression analysis of CTCs, but the technical difficulty of isolating and analyzing individual CTCs has limited progress in this area. To assess the ability of a new generation of MagSweeper to isolate intact CTCs for downstream analysis, we performed mRNA-Seq on single CTCs isolated from the blood of patients with metastatic prostate cancer and on single prostate cancer cell line LNCaP cells spiked into the blood of healthy donors. We found that the MagSweeper effectively isolated CTCs with a capture efficiency that matched the CellSearch platform. However, unlike CellSearch, the MagSweeper facilitates isolation of individual live CTCs without contaminating leukocytes. Importantly, mRNA-Seq analysis showed that the MagSweeper isolation process did not have a discernible impact on the transcriptional profile of single LNCaPs isolated from spiked human blood, suggesting that any perturbations caused by the MagSweeper process on the transcriptional signature of isolated cells are modest. Although the RNA from patient CTCs showed signs of significant degradation, consistent with reports of short half-lives and apoptosis amongst CTCs, transcriptional signatures of prostate tissue and of cancer were readily detectable with single CTC mRNA-Seq. These results demonstrate that the MagSweeper provides access to intact CTCs and that these CTCs can potentially supply clinically relevant information.Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in metastatic prostate cancer patients. Some evidence suggests that it is possible to derive additional information about tumors from expression analysis of CTCs, but the technical difficulty of isolating and analyzing individual CTCs has limited progress in this area. To assess the ability of a new generation of MagSweeper to isolate intact CTCs for downstream analysis, we performed mRNA-Seq on single CTCs isolated from the blood of patients with metastatic prostate cancer and on single prostate cancer cell line LNCaP cells spiked into the blood of healthy donors. We found that the MagSweeper effectively isolated CTCs with a capture efficiency that matched the CellSearch platform. However, unlike CellSearch, the MagSweeper facilitates isolation of individual live CTCs without contaminating leukocytes. Importantly, mRNA-Seq analysis showed that the MagSweeper isolation process did not have a discernible impact on the transcriptional profile of single LNCaPs isolated from spiked human blood, suggesting that any perturbations caused by the MagSweeper process on the transcriptional signature of isolated cells are modest. Although the RNA from patient CTCs showed signs of significant degradation, consistent with reports of short half-lives and apoptosis amongst CTCs, transcriptional signatures of prostate tissue and of cancer were readily detectable with single CTC mRNA-Seq. These results demonstrate that the MagSweeper provides access to intact CTCs and that these CTCs can potentially supply clinically relevant information.
Audience	Academic
Author	Ronaghi, Mostafa Luo, Shujun Talasaz, AmirAli H. Tat, Mai Li, Robin Stuart, Sarah Srinivas, Sandhya Cann, Gordon M. Cooper, Samantha Brooks, James D. Gulzar, Zulfiqar G. Schroth, Gary
AuthorAffiliation	3 Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, California, United States of America University of Kentucky College of Medicine, United States of America 2 Department of Urology, Stanford University Medical Center, Stanford, California, United States of America 1 Department of Diagnostic Research, Illumina, Inc., Hayward, California, United States of America
AuthorAffiliation_xml	– name: University of Kentucky College of Medicine, United States of America – name: 2 Department of Urology, Stanford University Medical Center, Stanford, California, United States of America – name: 1 Department of Diagnostic Research, Illumina, Inc., Hayward, California, United States of America – name: 3 Department of Medicine, Division of Oncology, Stanford University Medical Center, Stanford, California, United States of America
Author_xml	– sequence: 1 givenname: Gordon M. surname: Cann fullname: Cann, Gordon M. – sequence: 2 givenname: Zulfiqar G. surname: Gulzar fullname: Gulzar, Zulfiqar G. – sequence: 3 givenname: Samantha surname: Cooper fullname: Cooper, Samantha – sequence: 4 givenname: Robin surname: Li fullname: Li, Robin – sequence: 5 givenname: Shujun surname: Luo fullname: Luo, Shujun – sequence: 6 givenname: Mai surname: Tat fullname: Tat, Mai – sequence: 7 givenname: Sarah surname: Stuart fullname: Stuart, Sarah – sequence: 8 givenname: Gary surname: Schroth fullname: Schroth, Gary – sequence: 9 givenname: Sandhya surname: Srinivas fullname: Srinivas, Sandhya – sequence: 10 givenname: Mostafa surname: Ronaghi fullname: Ronaghi, Mostafa – sequence: 11 givenname: James D. surname: Brooks fullname: Brooks, James D. – sequence: 12 givenname: AmirAli H. surname: Talasaz fullname: Talasaz, AmirAli H.
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/23145101$$D View this record in MEDLINE/PubMed
BookMark	eNqNk11v0zAUhiM0xD7gHyCIhITgosUfaT64QKqqbVSa2NQObi3HPWk9JXYXO2P7C_xqTtpsaqoJoVw4Pn7Oa_v4PcfBgbEGguAtJUPKE_rlxja1keVwjeEhIVFGo-hFcEQzzgYxI_xg5_8wOHbuhpART-P4VXDIOI1GlNCj4E81-zEezOE2tEU412ZZQnhVW-elh3AijYI6nOhaNaX0uBpeN5XFCJSlC2dwB3IzKrnWfoNY0wqdg4Hw9H5dg3NtSJpFeCX96rd8cOGZbXCqzf4-r4OXBcrBm248CX6enV5Pvg8uLs-nk_HFQCWj1A8oHn5BVKRymjFWFDkFlXAoUqWoJFnEISU4ixPJo5zGKSeKLtIigjSlVOXAT4L3W911aZ3oyugE5SxOOOcjisR0SyysvBHrWleyfhBWarEJ2HopZO21KkFwmUEW5ylhBYu4ivMMIMlylgFhozzjqPWt263JK1goML6WZU-0v2L0SiztneBRxjhjKPCpE6jtbQPOi0o7hQ8gDdgGz01HWAi0RILohz30-dt11FLiBbQpLO6rWlExjpKEZDRN23MPn6HwW0ClFXqu0BjvJXzuJSDj4d4vZeOcmM5n_89e_uqzH3fYFTrOr5wtm9Zrrg--2630U4kfzY5AtAUUGs_VUDwhlIi2px7LJdqeEl1PYdrXvTSl_cbqWBFd_jv5L2IdJ70
CitedBy_id	crossref_primary_10_1016_j_gde_2013_12_004 crossref_primary_10_1016_j_biotechadv_2018_03_007 crossref_primary_10_1186_s13059_016_0941_0 crossref_primary_10_1002_pros_24704 crossref_primary_10_3109_10408363_2015_1023430 crossref_primary_10_1002_adtp_202400438 crossref_primary_10_1016_j_clcc_2013_11_003 crossref_primary_10_1186_s40169_017_0150_9 crossref_primary_10_1371_journal_pone_0139484 crossref_primary_10_1053_j_seminoncol_2013_04_008 crossref_primary_10_1002_ijc_32915 crossref_primary_10_3390_cancers12061376 crossref_primary_10_1124_mol_116_108142 crossref_primary_10_1002_adbi_201900164 crossref_primary_10_1186_1471_2199_14_6 crossref_primary_10_1146_annurev_pathol_052016_100256 crossref_primary_10_1002_cncr_31161 crossref_primary_10_1158_1078_0432_CCR_13_2664 crossref_primary_10_1038_s41598_018_30047_y crossref_primary_10_1101_gr_161034_113 crossref_primary_10_1016_j_omto_2021_04_001 crossref_primary_10_1373_clinchem_2014_229476 crossref_primary_10_1002_smll_202205856 crossref_primary_10_3390_ijms17091580 crossref_primary_10_2217_bmm_2016_0192 crossref_primary_10_1016_j_pharmthera_2013_12_011 crossref_primary_10_1016_j_tcb_2018_05_004 crossref_primary_10_1371_journal_pone_0130418 crossref_primary_10_1038_srep43710 crossref_primary_10_1063_1_4908049 crossref_primary_10_1039_C7AN01979C crossref_primary_10_3390_cancers13050970 crossref_primary_10_1186_s13059_016_0927_y crossref_primary_10_1016_j_aca_2021_338423 crossref_primary_10_1007_s00018_014_1601_8 crossref_primary_10_1016_j_bbcan_2024_189236 crossref_primary_10_1038_nbt_2892 crossref_primary_10_3390_cancers11010019 crossref_primary_10_1039_C7CS00016B crossref_primary_10_1063_1_4890466 crossref_primary_10_1126_scitranslmed_aav2169 crossref_primary_10_1586_14737159_2016_1116942 crossref_primary_10_3390_cancers12123525 crossref_primary_10_1016_j_juro_2018_01_033 crossref_primary_10_3390_diagnostics10080566 crossref_primary_10_1016_S0007_4551_16_30144_8 crossref_primary_10_1007_s11912_018_0683_0 crossref_primary_10_3390_diagnostics10080520 crossref_primary_10_1158_1078_0432_CCR_17_2312 crossref_primary_10_1038_s41467_017_01705_y crossref_primary_10_1016_j_coche_2014_01_011 crossref_primary_10_1586_14789450_2013_858021 crossref_primary_10_1586_14737159_2013_811896 crossref_primary_10_1073_pnas_1404656111 crossref_primary_10_1158_2159_8290_CD_13_1014 crossref_primary_10_1007_s10565_017_9418_5 crossref_primary_10_1002_ijc_28516 crossref_primary_10_1186_1479_5876_12_143 crossref_primary_10_1038_nrclinonc_2014_82 crossref_primary_10_1155_2021_9919080 crossref_primary_10_1038_nrclinonc_2013_253 crossref_primary_10_1038_nprot_2017_003 crossref_primary_10_1093_pnasnexus_pgae002 crossref_primary_10_1016_j_it_2016_12_001 crossref_primary_10_1016_j_jgg_2014_09_005 crossref_primary_10_1080_14737159_2018_1425143 crossref_primary_10_1016_j_biotechadv_2013_08_016 crossref_primary_10_1186_s12967_016_0829_5 crossref_primary_10_1007_s11864_013_0253_5 crossref_primary_10_1016_j_eururo_2020_12_037 crossref_primary_10_1038_s41598_017_11119_x crossref_primary_10_1016_j_critrevonc_2015_10_010 crossref_primary_10_3390_ijms241512337 crossref_primary_10_1007_s10555_014_9503_7 crossref_primary_10_3390_diagnostics5040428 crossref_primary_10_1038_s42003_021_02937_x crossref_primary_10_3390_cancers5041676 crossref_primary_10_1007_s40291_014_0101_8 crossref_primary_10_1053_j_gastro_2016_05_052 crossref_primary_10_1002_cam4_70902 crossref_primary_10_1038_s41698_019_0095_0 crossref_primary_10_3390_cancers12051135 crossref_primary_10_1016_j_molonc_2016_01_007 crossref_primary_10_1080_14737159_2018_1522251
Cites_doi	10.1371/journal.pone.0020293 10.1073/pnas.95.8.4589 10.1126/scitranslmed.3001498 10.1515/CCLM.2011.628 10.1200/JCO.2005.08.140 10.1158/1078-0432.CCR-10-1449 10.1016/j.cell.2011.02.013 10.1186/bcr2326 10.1002/cyto.a.20718 10.1126/scitranslmed.3000403 10.1093/nar/gkg115 10.1002/pros.10290 10.1073/pnas.95.25.14863 10.1002/cyto.a.20073 10.1097/PPO.0b013e31823e69ac 10.1371/journal.pone.0012517 10.1016/j.cell.2011.09.024 10.1073/pnas.1012539107 10.1002/pros.20312 10.1096/fj.02-0392fje 10.1016/j.humpath.2006.08.027 10.1126/science.1203543 10.1074/jbc.C600164200 10.1186/1471-2407-12-78 10.1093/bioinformatics/btp616 10.1200/JCO.1995.13.5.1195 10.1371/journal.pone.0033788 10.1158/1078-0432.CCR-07-1506 10.1056/NEJMoa040766 10.1126/scitranslmed.3000702 10.1038/sj.onc.1208490 10.1016/S0002-9440(10)64706-2 10.1056/NEJMoa0800668 10.1200/JCO.2007.15.8923 10.1038/nature06385 10.1073/pnas.0813188106 10.1371/journal.pone.0021366 10.1016/S0002-9440(10)61667-7 10.1038/sj.bjc.6604545 10.1158/0008-5472.CAN-04-4330 10.1038/nature09807 10.1002/pros.20050 10.1016/j.canlet.2008.12.007 10.1158/0008-5472.CAN-08-3667
ContentType	Journal Article
Copyright	COPYRIGHT 2012 Public Library of Science 2012 Cann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2012 Cann et al 2012 Cann et al
Copyright_xml	– notice: COPYRIGHT 2012 Public Library of Science – notice: 2012 Cann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2012 Cann et al 2012 Cann et al
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0049144
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest SciTech Premium Collection Technology Collection Materials Science & Engineering Database ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Local Electronic Collection Information Biological Science Collection ProQuest Central Technology collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE Agricultural Science Database MEDLINE - Academic
Database_xml	– sequence: 1 dbid: DOA name: Directory of Open Access Journals (DOAJ) url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General) Medicine Engineering Biology
DocumentTitleAlternate	mRNA-Seq of Human Circulating Tumor Cells
EISSN	1932-6203
ExternalDocumentID	1326733351 oai_doaj_org_article_3a9e96b802f243c6b9ee79b29e025b93 PMC3492322 2944419291 A477091883 23145101 10_1371_journal_pone_0049144
Genre	Research Support, U.S. Gov't, Non-P.H.S Journal Article
GeographicLocations	United States--US California
GeographicLocations_xml	– name: United States--US – name: California
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PYCSY RIG RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM ALIPV BBORY CGR CUY CVF ECM EIF NPM PV9 RZL 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 5PM AAPBV ABPTK N95
ID	FETCH-LOGICAL-c758t-1231d0c4cb1922ffb1ec73ef8cc1a0943e80ef867a34b16830c1d8f4e8811cbe3
IEDL.DBID	7RV
ISICitedReferencesCount	119
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000311935800219&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1932-6203
IngestDate	Sun May 07 16:28:42 EDT 2023 Fri Oct 03 12:33:55 EDT 2025 Tue Nov 04 02:01:25 EST 2025 Mon Sep 08 06:06:16 EDT 2025 Tue Oct 07 07:11:28 EDT 2025 Sat Nov 29 13:24:28 EST 2025 Sat Nov 29 10:13:39 EST 2025 Wed Nov 26 10:10:52 EST 2025 Wed Nov 26 10:29:24 EST 2025 Thu May 22 21:23:13 EDT 2025 Thu Apr 03 06:53:43 EDT 2025 Sat Nov 29 05:59:28 EST 2025 Tue Nov 18 22:28:47 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	11
Language	English
License	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c758t-1231d0c4cb1922ffb1ec73ef8cc1a0943e80ef867a34b16830c1d8f4e8811cbe3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: AT JDB GMC ZGG. Performed the experiments: GMC ZGG RL SL MT SS. Analyzed the data: SC AT GMC ZGG MR JDB. Contributed reagents/materials/analysis tools: GS SS JDB AT. Wrote the paper: GMC AT ZGG JDB. Competing Interests: The authors have read the journal's policy and have the following conflicts: GMC, SC, RL, SL, MT, SS, GS, MR, and AT are currently employed by Illumina. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
OpenAccessLink	https://www.proquest.com/docview/1326733351?pq-origsite=%requestingapplication%
PMID	23145101
PQID	1326733351
PQPubID	1436336
PageCount	e49144
ParticipantIDs	plos_journals_1326733351 doaj_primary_oai_doaj_org_article_3a9e96b802f243c6b9ee79b29e025b93 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3492322 proquest_miscellaneous_1151921377 proquest_journals_1326733351 gale_infotracmisc_A477091883 gale_infotracacademiconefile_A477091883 gale_incontextgauss_ISR_A477091883 gale_incontextgauss_IOV_A477091883 gale_healthsolutions_A477091883 pubmed_primary_23145101 crossref_primary_10_1371_journal_pone_0049144 crossref_citationtrail_10_1371_journal_pone_0049144
PublicationCentury	2000
PublicationDate	2012-11-07
PublicationDateYYYYMMDD	2012-11-07
PublicationDate_xml	– month: 11 year: 2012 text: 2012-11-07 day: 07
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: San Francisco – name: San Francisco, USA
PublicationTitle	PloS one
PublicationTitleAlternate	PLoS One
PublicationYear	2012
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	SL Stott (ref13) 2010; 107 S Nagrath (ref12) 2007; 450 S Malhotra (ref40) 2011; 6 W Weichert (ref39) 2004; 60 RJ Leary (ref24) 2010; 2 AA Powell (ref23) 2012; 7 A van Bokhoven (ref29) 2003; 57 E Racila (ref15) 1998; 95 M Cristofanilli (ref16) 2004; 351 JF Swennenhuis (ref37) 2009; 75 MJ Del Prete (ref35) 2002; 16 CJ Larson (ref34) 2004; 62 B Ateeq (ref30) 2011; 3 SL Stott (ref8) 2010; 2 D Marrinucci (ref19) 2007; 38 ZG Gulzar (ref31) 2012 AH Talasaz (ref22) 2009; 106 MD Robinson (ref27) 2010; 26 T Hudolin (ref41) 2006; 66 MJ Magbanua (ref21) 2012; 12 G Mehes (ref33) 2001; 159 MB Eisen (ref26) 1998; 95 C Alix-Panabieres (ref18) 2009; 11 TR Ashworth (ref4) 1869; 14 N Navin (ref25) 2011; 472 PD Thomas (ref28) 2003; 31 RA Ghossein (ref14) 1995; 13 L von Boehmer (ref42) 2011; 6 R Colnaghi (ref43) 2006; 281 CL Chaffer (ref1) 2011; 331 DC Danila (ref9) 2011; 17 G Vona (ref11) 2000; 156 E Rossi (ref38) 2010; 16 S Valastyan (ref2) 2011; 147 DA Smirnov (ref46) 2005; 65 PL Paris (ref17) 2009; 277 ES Lianidou (ref10) 2011; 49 SJ Cohen (ref6) 2008; 26 DC Danila (ref7) 2007; 13 D Hanahan (ref3) 2011; 144 TG Ntouroupi (ref20) 2008; 99 G Attard (ref36) 2009; 69 EA Punnoose (ref32) 2010; 5 M Zhang (ref44) 2005; 24 S Maheswaran (ref45) 2008; 359 M Cristofanilli (ref5) 2005; 23 7537803 - J Clin Oncol. 1995 May;13(5):1195-200 12397088 - FASEB J. 2002 Dec;16(14):2003-5 19549321 - Breast Cancer Res. 2009;11(3):R39 22373240 - BMC Cancer. 2012;12:78 15317891 - N Engl J Med. 2004 Aug 19;351(8):781-91 19162393 - Cancer Lett. 2009 May 18;277(2):164-73 21629784 - PLoS One. 2011;6(5):e20293 16114059 - Prostate. 2006 Jan 1;66(1):13-8 18097410 - Nature. 2007 Dec 20;450(7173):1235-9 21368222 - Sci Transl Med. 2011 Mar 2;3(72):72ra17 20978147 - Clin Cancer Res. 2010 Nov 1;16(21):5233-43 20371490 - Sci Transl Med. 2010 Feb 24;2(20):20ra14 21376230 - Cell. 2011 Mar 4;144(5):646-74 18682708 - Br J Cancer. 2008 Sep 2;99(5):789-95 19234122 - Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3970-5 22000009 - Cell. 2011 Oct 14;147(2):275-92 21436443 - Science. 2011 Mar 25;331(6024):1559-64 15735118 - J Clin Oncol. 2005 Mar 1;23(7):1420-30 9843981 - Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 15958538 - Cancer Res. 2005 Jun 15;65(12):4993-7 10623654 - Am J Pathol. 2000 Jan;156(1):57-63 19910308 - Bioinformatics. 2010 Jan 1;26(1):139-40 20838621 - PLoS One. 2010;5(9):e12517 22349817 - Oncogene. 2013 Jan 3;32(1):70-7 20424012 - Sci Transl Med. 2010 Mar 31;2(25):25ra23 15735703 - Oncogene. 2005 Apr 7;24(15):2474-82 21801030 - Clin Chem Lab Med. 2011 Oct;49(10):1579-90 20930119 - Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18392-7 21754986 - PLoS One. 2011;6(7):e21366 22157288 - Cancer J. 2011 Nov-Dec;17(6):438-50 21399628 - Nature. 2011 Apr 7;472(7341):90-4 18596266 - N Engl J Med. 2008 Jul 24;359(4):366-77 15176053 - Prostate. 2004 Aug 1;60(3):240-5 11438448 - Am J Pathol. 2001 Jul;159(1):17-20 19291800 - Cytometry A. 2009 Jun;75(6):520-7 14518029 - Prostate. 2003 Nov 1;57(3):205-25 16950794 - J Biol Chem. 2006 Nov 3;281(44):33450-6 18591556 - J Clin Oncol. 2008 Jul 1;26(19):3213-21 17188328 - Hum Pathol. 2007 Mar;38(3):514-9 22586443 - PLoS One. 2012;7(5):e33788 19339269 - Cancer Res. 2009 Apr 1;69(7):2912-8 18056182 - Clin Cancer Res. 2007 Dec 1;13(23):7053-8 9539782 - Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4589-94 15472900 - Cytometry A. 2004 Nov;62(1):46-53 12520017 - Nucleic Acids Res. 2003 Jan 1;31(1):334-41
References_xml	– volume: 6 start-page: e20293 year: 2011 ident: ref40 article-title: A tri-marker proliferation index predicts biochemical recurrence after surgery for prostate cancer publication-title: PLoS One doi: 10.1371/journal.pone.0020293 – volume: 95 start-page: 4589 year: 1998 ident: ref15 article-title: Detection and characterization of carcinoma cells in the blood publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.95.8.4589 – volume: 14 start-page: 146 year: 1869 ident: ref4 article-title: A case of cancer in which cells similar to those in the tumors were seen in the blood after death publication-title: Aust Med J – volume: 3 start-page: 72ra17 year: 2011 ident: ref30 article-title: Therapeutic targeting of SPINK1-positive prostate cancer publication-title: Sci Transl Med doi: 10.1126/scitranslmed.3001498 – volume: 49 start-page: 1579 year: 2011 ident: ref10 article-title: Circulating tumor cells as emerging tumor biomarkers in breast cancer publication-title: Clin Chem Lab Med doi: 10.1515/CCLM.2011.628 – volume: 23 start-page: 1420 year: 2005 ident: ref5 article-title: Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer publication-title: J Clin Oncol doi: 10.1200/JCO.2005.08.140 – volume: 16 start-page: 5233 year: 2010 ident: ref38 article-title: M30 neoepitope expression in epithelial cancer: quantification of apoptosis in circulating tumor cells by CellSearch analysis publication-title: Clin Cancer Res doi: 10.1158/1078-0432.CCR-10-1449 – volume: 144 start-page: 646 year: 2011 ident: ref3 article-title: Hallmarks of cancer: the next generation publication-title: Cell doi: 10.1016/j.cell.2011.02.013 – volume: 11 start-page: R39 year: 2009 ident: ref18 article-title: Full-length cytokeratin-19 is released by human tumor cells: a potential role in metastatic progression of breast cancer publication-title: Breast Cancer Res doi: 10.1186/bcr2326 – volume: 75 start-page: 520 year: 2009 ident: ref37 article-title: Characterization of circulating tumor cells by fluorescence in situ hybridization publication-title: Cytometry A doi: 10.1002/cyto.a.20718 – volume: 2 start-page: 25ra23 year: 2010 ident: ref8 article-title: Isolation and characterization of circulating tumor cells from patients with localized and metastatic prostate cancer publication-title: Sci Transl Med doi: 10.1126/scitranslmed.3000403 – volume: 31 start-page: 334 year: 2003 ident: ref28 article-title: PANTHER: a browsable database of gene products organized by biological function, using curated protein family and subfamily classification publication-title: Nucleic Acids Res doi: 10.1093/nar/gkg115 – volume: 57 start-page: 205 year: 2003 ident: ref29 article-title: Molecular characterization of human prostate carcinoma cell lines publication-title: Prostate doi: 10.1002/pros.10290 – volume: 95 start-page: 14863 year: 1998 ident: ref26 article-title: Cluster analysis and display of genome-wide expression patterns publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.95.25.14863 – volume: 62 start-page: 46 year: 2004 ident: ref34 article-title: Apoptosis of circulating tumor cells in prostate cancer patients publication-title: Cytometry A doi: 10.1002/cyto.a.20073 – volume: 17 start-page: 438 year: 2011 ident: ref9 article-title: Circulating tumors cells as biomarkers: progress toward biomarker qualification publication-title: Cancer J doi: 10.1097/PPO.0b013e31823e69ac – volume: 5 start-page: e12517 year: 2010 ident: ref32 article-title: Molecular biomarker analyses using circulating tumor cells publication-title: PLoS One doi: 10.1371/journal.pone.0012517 – volume: 147 start-page: 275 year: 2011 ident: ref2 article-title: Tumor metastasis: molecular insights and evolving paradigms publication-title: Cell doi: 10.1016/j.cell.2011.09.024 – volume: 107 start-page: 18392 year: 2010 ident: ref13 article-title: Isolation of circulating tumor cells using a microvortex-generating herringbone-chip publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1012539107 – volume: 66 start-page: 13 year: 2006 ident: ref41 article-title: Immunohistochemical expression of tumor antigens MAGE-A1, MAGE-A3/4, and NY-ESO-1 in cancerous and benign prostatic tissue publication-title: Prostate doi: 10.1002/pros.20312 – volume: 16 start-page: 2003 year: 2002 ident: ref35 article-title: Degradation of cellular mRNA is a general early apoptosis-induced event publication-title: FASEB J doi: 10.1096/fj.02-0392fje – volume: 38 start-page: 514 year: 2007 ident: ref19 article-title: Case study of the morphologic variation of circulating tumor cells publication-title: Hum Pathol doi: 10.1016/j.humpath.2006.08.027 – volume: 331 start-page: 1559 year: 2011 ident: ref1 article-title: A perspective on cancer cell metastasis publication-title: Science doi: 10.1126/science.1203543 – volume: 281 start-page: 33450 year: 2006 ident: ref43 article-title: Separating the anti-apoptotic and mitotic roles of survivin publication-title: J Biol Chem doi: 10.1074/jbc.C600164200 – volume: 12 start-page: 78 year: 2012 ident: ref21 article-title: Isolation and genomic analysis of circulating tumor cells from castration resistant metastatic prostate cancer publication-title: BMC Cancer doi: 10.1186/1471-2407-12-78 – volume: 26 start-page: 139 year: 2010 ident: ref27 article-title: edgeR: a Bioconductor package for differential expression analysis of digital gene expression data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp616 – volume: 13 start-page: 1195 year: 1995 ident: ref14 article-title: Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: clinical implications publication-title: J Clin Oncol doi: 10.1200/JCO.1995.13.5.1195 – volume: 7 start-page: e33788 year: 2012 ident: ref23 article-title: Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines publication-title: PLoS One doi: 10.1371/journal.pone.0033788 – volume: 13 start-page: 7053 year: 2007 ident: ref7 article-title: Circulating tumor cell number and prognosis in progressive castration-resistant prostate cancer publication-title: Clin Cancer Res doi: 10.1158/1078-0432.CCR-07-1506 – volume: 351 start-page: 781 year: 2004 ident: ref16 article-title: Circulating tumor cells, disease progression, and survival in metastatic breast cancer publication-title: N Engl J Med doi: 10.1056/NEJMoa040766 – volume: 2 start-page: 20ra14 year: 2010 ident: ref24 article-title: Development of personalized tumor biomarkers using massively parallel sequencing publication-title: Sci Transl Med doi: 10.1126/scitranslmed.3000702 – volume: 24 start-page: 2474 year: 2005 ident: ref44 article-title: Survivin mediates resistance to antiandrogen therapy in prostate cancer publication-title: Oncogene doi: 10.1038/sj.onc.1208490 – volume: 156 start-page: 57 year: 2000 ident: ref11 article-title: Isolation by size of epithelial tumor cells : a new method for the immunomorphological and molecular characterization of circulatingtumor cells publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)64706-2 – volume: 359 start-page: 366 year: 2008 ident: ref45 article-title: Detection of mutations in EGFR in circulating lung-cancer cells publication-title: N Engl J Med doi: 10.1056/NEJMoa0800668 – volume: 26 start-page: 3213 year: 2008 ident: ref6 article-title: Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer publication-title: J Clin Oncol doi: 10.1200/JCO.2007.15.8923 – volume: 450 start-page: 1235 year: 2007 ident: ref12 article-title: Isolation of rare circulating tumour cells in cancer patients by microchip technology publication-title: Nature doi: 10.1038/nature06385 – volume: 106 start-page: 3970 year: 2009 ident: ref22 article-title: Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0813188106 – volume: 6 start-page: e21366 year: 2011 ident: ref42 article-title: MAGE-C2/CT10 protein expression is an independent predictor of recurrence in prostate cancer publication-title: PLoS One doi: 10.1371/journal.pone.0021366 – volume: 159 start-page: 17 year: 2001 ident: ref33 article-title: Circulating breast cancer cells are frequently apoptotic publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)61667-7 – volume: 99 start-page: 789 year: 2008 ident: ref20 article-title: Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope publication-title: Br J Cancer doi: 10.1038/sj.bjc.6604545 – year: 2012 ident: ref31 article-title: Increased expression of NuSAP in recurrent prostate cancer is mediated by E2F1 publication-title: Oncogene – volume: 65 start-page: 4993 year: 2005 ident: ref46 article-title: Global gene expression profiling of circulating tumor cells publication-title: Cancer Res doi: 10.1158/0008-5472.CAN-04-4330 – volume: 472 start-page: 90 year: 2011 ident: ref25 article-title: Tumour evolution inferred by single-cell sequencing publication-title: Nature doi: 10.1038/nature09807 – volume: 60 start-page: 240 year: 2004 ident: ref39 article-title: Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades publication-title: Prostate doi: 10.1002/pros.20050 – volume: 277 start-page: 164 year: 2009 ident: ref17 article-title: Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer publication-title: Cancer Lett doi: 10.1016/j.canlet.2008.12.007 – volume: 69 start-page: 2912 year: 2009 ident: ref36 article-title: Characterization of ERG, AR and PTEN gene status in circulating tumor cells from patients with castration-resistant prostate cancer publication-title: Cancer Res doi: 10.1158/0008-5472.CAN-08-3667 – reference: 15317891 - N Engl J Med. 2004 Aug 19;351(8):781-91 – reference: 21801030 - Clin Chem Lab Med. 2011 Oct;49(10):1579-90 – reference: 20424012 - Sci Transl Med. 2010 Mar 31;2(25):25ra23 – reference: 21436443 - Science. 2011 Mar 25;331(6024):1559-64 – reference: 17188328 - Hum Pathol. 2007 Mar;38(3):514-9 – reference: 12520017 - Nucleic Acids Res. 2003 Jan 1;31(1):334-41 – reference: 19549321 - Breast Cancer Res. 2009;11(3):R39 – reference: 15735703 - Oncogene. 2005 Apr 7;24(15):2474-82 – reference: 19291800 - Cytometry A. 2009 Jun;75(6):520-7 – reference: 10623654 - Am J Pathol. 2000 Jan;156(1):57-63 – reference: 16950794 - J Biol Chem. 2006 Nov 3;281(44):33450-6 – reference: 20978147 - Clin Cancer Res. 2010 Nov 1;16(21):5233-43 – reference: 22157288 - Cancer J. 2011 Nov-Dec;17(6):438-50 – reference: 21376230 - Cell. 2011 Mar 4;144(5):646-74 – reference: 15472900 - Cytometry A. 2004 Nov;62(1):46-53 – reference: 21368222 - Sci Transl Med. 2011 Mar 2;3(72):72ra17 – reference: 9539782 - Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4589-94 – reference: 19910308 - Bioinformatics. 2010 Jan 1;26(1):139-40 – reference: 16114059 - Prostate. 2006 Jan 1;66(1):13-8 – reference: 20371490 - Sci Transl Med. 2010 Feb 24;2(20):20ra14 – reference: 22373240 - BMC Cancer. 2012;12:78 – reference: 19234122 - Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3970-5 – reference: 21754986 - PLoS One. 2011;6(7):e21366 – reference: 18056182 - Clin Cancer Res. 2007 Dec 1;13(23):7053-8 – reference: 20930119 - Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18392-7 – reference: 22349817 - Oncogene. 2013 Jan 3;32(1):70-7 – reference: 15735118 - J Clin Oncol. 2005 Mar 1;23(7):1420-30 – reference: 19339269 - Cancer Res. 2009 Apr 1;69(7):2912-8 – reference: 20838621 - PLoS One. 2010;5(9):e12517 – reference: 12397088 - FASEB J. 2002 Dec;16(14):2003-5 – reference: 9843981 - Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 – reference: 15958538 - Cancer Res. 2005 Jun 15;65(12):4993-7 – reference: 18591556 - J Clin Oncol. 2008 Jul 1;26(19):3213-21 – reference: 18596266 - N Engl J Med. 2008 Jul 24;359(4):366-77 – reference: 19162393 - Cancer Lett. 2009 May 18;277(2):164-73 – reference: 22586443 - PLoS One. 2012;7(5):e33788 – reference: 15176053 - Prostate. 2004 Aug 1;60(3):240-5 – reference: 22000009 - Cell. 2011 Oct 14;147(2):275-92 – reference: 11438448 - Am J Pathol. 2001 Jul;159(1):17-20 – reference: 18097410 - Nature. 2007 Dec 20;450(7173):1235-9 – reference: 18682708 - Br J Cancer. 2008 Sep 2;99(5):789-95 – reference: 14518029 - Prostate. 2003 Nov 1;57(3):205-25 – reference: 21399628 - Nature. 2011 Apr 7;472(7341):90-4 – reference: 21629784 - PLoS One. 2011;6(5):e20293 – reference: 7537803 - J Clin Oncol. 1995 May;13(5):1195-200
SSID	ssj0053866
Score	2.4378777
Snippet	Circulating tumor cells (CTC) mediate metastatic spread of many solid tumors and enumeration of CTCs is currently used as a prognostic indicator of survival in...
SourceID	plos doaj pubmedcentral proquest gale pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	e49144
SubjectTerms	Analysis Apoptosis Biology Biomarkers, Tumor - blood Blood Cancer Cancer genetics Cancer metastasis Cell Line, Tumor Engineering Enumeration Gene expression Gene Expression Regulation, Neoplastic Human subjects Humans Kinases Leukocytes Male Medicine Messenger RNA Metabolic Networks and Pathways Metastases Metastasis Neoplasm Metastasis - genetics Neoplasm Metastasis - pathology Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Patients Prognosis Prostate cancer Prostatic Neoplasms - blood Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Ribonucleic acid RNA RNA, Messenger - blood RNA, Messenger - genetics Sequence Alignment Sequence Analysis, RNA Solid tumors Transcription Transcription (Genetics) Tumor cells Tumors Urology
SummonAdditionalLinks	– databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELdQxQMviPG1wgCDkICHbEnsxvZjQUwgoYI2QHuLbMcelbqkNC0f_wJ_NXeOGy3TpPHAU9X6krZ357uf27vfEfJcAYg3yFPpuBcJdwVLjPYsAaRbKYk514ZG4Q9iNpMnJ-rTuVFfWBPW0QN3ijtgWjlVGJnmPufMFkY5J5TJlYNsbVTg-UyF2h6muhgMu7goYqMcE9lBtMv-sqndPoJiOEYMElHg6--j8mi5aNrLIOfFyslzqejwFrkZMSSddp99h1xz9W2yE3dpS19GKulXd8ifs6PZNDl232njKf4qsHB0iX0egDCpRYOvqJ2vbJjhVZ_S9easgVfcYtFS5HbS4dHq5Xwdx3zhjcDnHHW_YgltTXVdUZxs_FP_bqnHOU10Xl98n7vky-Hbz2_eJXH6QmLhDLFOIKVlVWq5NQACc-9N5qxgzktrM431iE6m8KwQmnGTFZKlNquk507KLLPGsXtkVIO-dwlNJ7mWJteZ9pqnemIMY2mlnM-tVpxXY8K2pihtpCbHCRmLMvzfJuCI0mm2RAOW0YBjkvRXLTtqjivkX6OVe1kk1g4vgLuV0d3Kq9xtTJ6gj5Rdl2ofHsopFwKgl5Qg8SxIILlGjdU7p3rTtuX7j1__Qej4aCD0Igr5BtRhdeyYgO-EpF0Dyb2BJIQIO1jeRY_eaqUFHeWFYIxNMrhy6-WXLz_tl_GmWJFXu2YDMgAVVY58lWNyv9sUvWbBczjG-jERg-0yUP1wpZ5_C9zmSJYJOebB_7DVQ3ID4G0eOkfFHhmtVxv3iFy3P9bzdvU4BIy_2jFzcg priority: 102 providerName: Directory of Open Access Journals – databaseName: Public Library of Science (PLoS) Journals Open Access dbid: FPL link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3fb9MwELag8MALMH5shQEGIQEPGUmcxvZjqTaBNJWqHWhvke3Yo1KXlKYd8C_wV3OXuoFUm4CnqvHZTS8-33fK3XeEvJQA4jXyVNrE8SCxKQu0ciwApJtLgT7X1IXCx3w4FKencvQ7UNx6g8949Nbr9GBeFvYAAS2EANfJjZilKQZbR6PjzckLtpumvjzuqpkt91Oz9DdncWc-K6vLgOZ2vuQfDujozv_e-l1y20NN2l_vjR1yzRb3yI435oq-9ozTb-6Tn-fjYT-Y2K-0dHQCzmxm6QjLQQCI0gHuiwUdTBembvVVnNGT1XkJV-xsVtGxvQCsiZ9GzadL3w0MF8L16eF3n2lbUFXkdASA85v6UdG6nROdFtu_84B8Ojo8GbwPfJOGwECosQzA80V5aBKjASvGzunIGs6sE8ZECtMWrQjhW8oVS3SUChaaKBcusUJEkdGWPSSdAvSzR2jYi5XQsYqUU0moelozFubSutgomSR5l7DNs8uMZzDHRhqzrH4txyGSWWs2Q4VnXuFdEjSz5msGj7_Iv8Nt0cgi_3Z9AZ5s5s05Y0pamWoRxi5OmEm1tJZLHUsLGFJL1iXPcFNl62LW5hTJ-gnngNCEAIkXtQRycBSY5HOmVlWVffj4-R-EJuOW0Csv5EpQh1G-sAL-E3J7tST3W5JwkpjW8B6awEYrFegoTjljrBfBzI1ZXD78vBnGRTFxr7DlCmQAUcoYaS27ZHdtRY1mYeck6BK6hLfsq6X69kgx_VJToCOnJriiR1ff8WNyC7BtXJeN8n3SWS5W9gm5aS6W02rxtD43fgFAu24w priority: 102 providerName: Public Library of Science
Title	mRNA-Seq of Single Prostate Cancer Circulating Tumor Cells Reveals Recapitulation of Gene Expression and Pathways Found in Prostate Cancer
URI	https://www.ncbi.nlm.nih.gov/pubmed/23145101 https://www.proquest.com/docview/1326733351 https://www.proquest.com/docview/1151921377 https://pubmed.ncbi.nlm.nih.gov/PMC3492322 https://doaj.org/article/3a9e96b802f243c6b9ee79b29e025b93 http://dx.doi.org/10.1371/journal.pone.0049144
Volume	7
WOSCitedRecordID	wos000311935800219&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: Directory of Open Access Journals (DOAJ) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELdg44EXYHytMIZBSMBDtiROY-cJbdUmpm0lasdUeIlsxxmVuqRr2gH_An81d65byDQBEi9XNb64jX2--9m5D0JeJQDiFeapNFHBvcjEzFOyYB4g3TwRaHO1DRQ-4t2uGAyS1B241c6tcqETraLOK41n5Nuwa4o5Y6wdvBtfeFg1Ct-uuhIaN8lqgNgY5Jn3TheaGNZyHLtwOcaDbTc7W-OqNFsIjWEz0TBHNmv_UjevjEdVfR3wvOo_-ZtB2r_7v49yj9xxUJTuzGVnjdww5X2y5hZ7Td-4jNRvH5Af573ujtc3F7QqaB-M3cjQFMNFAKjSDsrNhHaGE21LgZVn9GR2XsEVMxrVtGcuAYvip5bj4dRVC8OOsH-698154pZUljlNAZB-ld9rass90WF59Xceko_7eyed954r4uBp2IpMPbCMQe7rSCvAkmFRqMBozkwhtA4kujUa4cO3mEsWqSAWzNdBLorICBEEWhn2iKyUMGHrhPrtUAoVykAWMvJlWynG_DwxRahlEkV5i7DFXGbaZTjHQhujzL6247DTmY9shhKQOQloEW9513ie4eMv_LsoJktezM9tL1STs8wt94zJxCSxEn5YhBHTsUqM4YkKEwMYUyWsRZ6jkGXzYNellsl2Is4BwQkBHC8tB-boKNEJ6EzO6jo7-HD6D0z9XoPptWMqKhgOLV3gBTwT5v5qcG40OEHT6EbzOi6JxajU2S9BhjsXon5984tlM3aKjn2lqWbAA4gzCTHtZYs8nq-q5ciC5ERoMlqEN9ZbY-ibLeXwi02Rjjk3wVQ9-fPfekpuA_4NbWgp3yAr08nMPCO39OV0WE82rS5BOuCWCqCiE2yS1d29btrbtMc3QPfTI6CHu1tAj_1DpDy1tA80bX-GO9KD4_TTT5EcjIc
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VgAQXoLwaKHRBIMrBre3d-HFAKIRWjRpC1ARUcTG763WIlNppnLT0L_Bj-I3MOOuAqwq49MApSna8SSbz-Dae-YaQ5yGAeIk8lZonvsW1xywpEmYB0o3DAHOuKhqFO363Gxwehr0V8qPshcGyyjImFoE6zhT-R74NpybPZ4w1nDeTYwunRuHd1XKExsIs9vXZKRzZ8tftd_D7vnDd3Z1Ba88yUwUsBdh4ZkGodmJbcSUB3LhJIh2tfKaTQClHYJ2dDmx45vmCcel4AbOVEwcJ10HgOEpqBvteIVc5d230ol7jcxn5IXZ4nmnPY76zbaxha5KleguhOBxeKumvmBKwzAW1yTjLLwK65-s1f0uAu7f-N9XdJjcN1KbNhW-skhWd3iGrJpjldNMwbr-6S74fHXSbVl8f0yyhfUjmY0172A4DQJy20C-mtDWaqmLUWTqkg_lRBq_o8TinB_oEsDY-KjEZzcw0NNwI96c730ylcUpFGtMeAO5TcZbTYpwVHaXn3-ce-XgpOrlPaikYyBqhdsMVgXSFIxLBbdGQkjE7DnXiKhFyHtcJK20nUobBHQeJjKPitqQPJ7mFZiO0uMhYXJ1Yy6smCwaTv8i_RbNcyiL_ePFCNh1GJpxFTIQ69GRgu4nLmfJkqLUfSjfUgKFlyOpkA406WjTzLqNo1OS-Dwg1CEDiWSGBHCQpFjkNxTzPo_aHT_8g1D-oCL00QkkG6lDCNJbAd0Jus4rkekUSIqmqLK-hC5ZayaNfjgNXlq518fLT5TJuioWLqc7mIAOIOnSR1rNOHiy8eKlZsByOKbFO_Ip_V1RfXUlHXwsKeOQUhVT88M8fa4Nc3xu870Sddnf_EbkBWN8t2mj9dVKbTef6MbmmTmajfPqkiGOUfLls7_8JTcHdkw
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VgBAXoLwaKHRBIODgxvY6XvuAUEgbEbUKUVJQxcXsrtchUmqncdLSv8BP4tcx46wDrirg0gOnyN7xOp7M45t4HoQ8DwHES-xTqb2EW572mSVFwixAunEYoM9VRaHwPu_1gsPDsL9GfpS1MJhWWdrEwlDHmcL_yBsQNfmcMdZ0GolJi-jvdN5Ojy2cIIVvWstxGksR2dNnpxC-5W-6O_Bbv3Ddzu5B-71lJgxYCnDy3AKz7cS28pQEoOMmiXS04kwngVKOwJw7Hdhw5HPBPOn4AbOVEweJp4PAcZTUDPa9Qq5yiDExnbDf_Fx6AbAjvm9K9Rh3GkYytqdZqrcRlkMgU3GFxcSAlV-oTSdZfhHoPZ-7-Zsz7Nz6n9l4m9w0EJy2ljqzTtZ0eoesGyOX01emE_fru-T70aDXsob6mGYJHYKTn2jaxzIZAOi0jfoyo-3xTBUj0NIRPVgcZXBGTyY5HegTwOD4qcR0PDdT0nAj3J_ufjMZyCkVaUz7AMRPxVlOizFXdJyev8898vFSeHKf1FIQlg1C7aYrAukKRyTCs0VTSsbsONSJq0ToeXGdsFKOImU6u-OAkUlUvK7kEOEtORuh9EVG-urEWl01XXY2-Qv9OxTRFS32JS9OZLNRZMxcxESoQ18Gtpu4HlO-DLXmoXRDDdhahqxOtlDAo2WR78q6Ri2Pc0CuQQAUzwoK7E2SoniOxCLPo-6HT_9ANBxUiF4aoiQDdihhCk7gmbDnWYVys0IJFlZVljdQHUuu5NEvJYIrSzW7ePnpahk3xYTGVGcLoAGkHbrY7rNOHiw1esVZkBwPXWWd8IquV1hfXUnHX4vW8NhrFFz0wz9_rS1yHZQ-2u_29h6RGxACuEV1Ld8ktflsoR-Ta-pkPs5nTwqTRsmXy1b-n2dt5l0
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=mRNA-Seq+of+Single+Prostate+Cancer+Circulating+Tumor+Cells+Reveals+Recapitulation+of+Gene+Expression+and+Pathways+Found+in+Prostate+Cancer&rft.jtitle=PloS+one&rft.au=Cann%2C+Gordon+M&rft.au=Gulzar%2C+Zulfiqar+G&rft.au=Cooper%2C+Samantha&rft.au=Li%2C+Robin&rft.date=2012-11-07&rft.pub=Public+Library+of+Science&rft.eissn=1932-6203&rft.volume=7&rft.issue=11&rft.spage=e49144&rft_id=info:doi/10.1371%2Fjournal.pone.0049144&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=2944419291
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon