Lactobacillus johnsonii N6.2 Mitigates the Development of Type 1 Diabetes in BB-DP Rats

The intestinal epithelium is a barrier that composes one of the most immunologically active surfaces of the body due to constant exposure to microorganisms as well as an infinite diversity of food antigens. Disruption of intestinal barrier function and aberrant mucosal immune activation have been im...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	PloS one Ročník 5; číslo 5; s. e10507
Hlavní autoři:	Valladares, Ricardo, Sankar, Dhyana, Li, Nan, Williams, Emily, Lai, Kin-Kwan, Abdelgeliel, Asmaa Sayed, Gonzalez, Claudio F., Wasserfall, Clive H., Larkin, Joseph, Schatz, Desmond, Atkinson, Mark A., Triplett, Eric W., Neu, Josef, Lorca, Graciela L.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 06.05.2010 Public Library of Science (PLoS)
Témata:	Aberration Alzheimer's disease Animals Antibiotics Antigens Bacteria Bacteroidetes Comparative analysis Cyclooxygenase-2 Cytokines Cytokines - genetics Cytokines - metabolism Diabetes Diabetes and Endocrinology/Type 1 Diabetes Diabetes mellitus Diabetes Mellitus, Type 1 - microbiology Diabetes Mellitus, Type 1 - pathology Digestive system Digestive tract Disruption Epithelium Feeding Behavior Female Firmicutes Gastrointestinal system Gastrointestinal tract Gastrointestinal Tract - microbiology Gastrointestinal Tract - pathology Gene expression Hypotheses Ileum Immune response Immunology Incidence Inflammation Inflammation Mediators - metabolism Intestinal microflora Intestine Kaplan-Meier Estimate Laboratories Lactobacillus Lactobacillus - physiology Male Membrane Proteins - metabolism Metabolism Metagenome Microbiology/Applied Microbiology Microbiology/Medical Microbiology Microbiota (Symbiotic organisms) Microorganisms Mucosal immunity Nitric-oxide synthase Oxidative stress Oxidative Stress - genetics Pathogenesis Pediatrics Prostate Proteins Rats Rats, Inbred BB RNA, Messenger - genetics RNA, Messenger - metabolism Rodents Science Tight Junctions - metabolism Type 1 diabetes Weaning γ-Interferon Florida United States > US
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	The intestinal epithelium is a barrier that composes one of the most immunologically active surfaces of the body due to constant exposure to microorganisms as well as an infinite diversity of food antigens. Disruption of intestinal barrier function and aberrant mucosal immune activation have been implicated in a variety of diseases within and outside of the gastrointestinal tract. With this model in mind, recent studies have shown a link between diet, composition of intestinal microbiota, and type 1 diabetes pathogenesis. In the BioBreeding rat model of type 1 diabetes, comparison of the intestinal microbial composition of diabetes prone and diabetes resistant animals found Lactobacillus species were negatively correlated with type 1 diabetes development. Two species, Lactobacillus johnsonii and L. reuteri, were isolated from diabetes resistant rats. In this study diabetes prone rats were administered pure cultures of L. johnsonii or L. reuteri isolated from diabetes resistant rats to determine the effect on type 1 diabetes development. Findings Results Rats administered L. johnsonii, but not L. reuteri, post-weaning developed type 1 diabetes at a protracted rate. Analysis of the intestinal ileum showed administration of L. johnsonii induced changes in the native microbiota, host mucosal proteins, and host oxidative stress response. A decreased oxidative intestinal environment was evidenced by decreased expression of several oxidative response proteins in the intestinal mucosa (Gpx1, GR, Cat). In L. johnsonii fed animals low levels of the pro-inflammatory cytokine IFNgamma were correlated with low levels of iNOS and high levels of Cox2. The administration of L. johnsonii also resulted in higher levels of the tight junction protein claudin. It was determined that the administration of L. johnsonii isolated from BioBreeding diabetes resistant rats delays or inhibits the onset of type 1 diabetes in BioBreeding diabetes prone rats. Taken collectively, these data suggest that the gut and the gut microbiota are potential agents of influence in type 1 diabetes development. These data also support therapeutic efforts that seek to modify gut microbiota as a means to modulate development of this disorder.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: CFG MAA JN GLL. Performed the experiments: RV DS NL EW ASA KKL CHW GLL. Analyzed the data: RV DS NL EW KKL CFG CHW JL DS MAA EWT JN GLL. Contributed reagents/materials/analysis tools: CHW DS MAA EWT JN GLL. Wrote the paper: RV CFG JN GLL.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0010507