Improving cold storage and processing traits in potato through targeted gene knockout

Cold storage of potato tubers is commonly used to reduce sprouting and extend postharvest shelf life. However, cold temperature stimulates the accumulation of reducing sugars in potato tubers. Upon high‐temperature processing, these reducing sugars react with free amino acids, resulting in brown, bi...

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Veröffentlicht in:	Plant biotechnology journal Jg. 14; H. 1; S. 169 - 176
Hauptverfasser:	Clasen, Benjamin M, Stoddard, Thomas J, Luo, Song, Demorest, Zachary L, Li, Jin, Cedrone, Frederic, Tibebu, Redeat, Davison, Shawn, Ray, Erin E, Daulhac, Aurelie, Coffman, Andrew, Yabandith, Ann, Retterath, Adam, Haun, William, Baltes, Nicholas J, Mathis, Luc, Voytas, Daniel F, Zhang, Feng
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	England Blackwell Pub 01.01.2016 John Wiley & Sons, Inc John Wiley and Sons Inc
Schlagworte:	Acrylamide Acrylamide - analysis acrylamide reduction acrylamides alleles Amino acids Autotetraploid Base Sequence beta-fructofuranosidase beta-Fructofuranosidase - genetics Bitter taste Carbohydrates - analysis Carcinogens Chips cold Cold storage Cold Temperature cold‐induced sweetening Cryopreservation - methods Deoxyribonucleic acid DNA free amino acids Fructose gene editing Gene expression Gene Knockout Techniques Gene silencing Gene Targeting Genes Genes, Plant Genomes Glucose Interference Invertase Mutation Mutation - genetics Mutations Nuclease nucleases Plants (organisms) Plants, Genetically Modified potato Potatoes reducing sugars Ribonucleic acid RNA RNA interference RNA-mediated interference Shelf life Silence Solanum tuberosum Solanum tuberosum - genetics sprouting Sucrose Sugar Sugars temperature Temperature effects Temporal variations Transcription Transcription activator-like effector nucleases Transcription Activator-Like Effector Nucleases - metabolism Transgenic plants Tubers vacuolar invertase vacuoles Vacuoles - enzymology Vegetables acrylamide reduction vacuolar invertase cold-induced sweetening gene editing transcription activator-like effector nucleases potato
ISSN:	1467-7644, 1467-7652, 1467-7652
Online-Zugang:	Volltext
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Zusammenfassung:	Cold storage of potato tubers is commonly used to reduce sprouting and extend postharvest shelf life. However, cold temperature stimulates the accumulation of reducing sugars in potato tubers. Upon high‐temperature processing, these reducing sugars react with free amino acids, resulting in brown, bitter‐tasting products and elevated levels of acrylamide—a potential carcinogen. To minimize the accumulation of reducing sugars, RNA interference (RNAi) technology was used to silence the vacuolar invertase gene (VInv), which encodes a protein that breaks down sucrose to glucose and fructose. Because RNAi often results in incomplete gene silencing and requires the plant to be transgenic, here we used transcription activator‐like effector nucleases (TALENs) to knockout VInv within the commercial potato variety, Ranger Russet. We isolated 18 plants containing mutations in at least one VInv allele, and five of these plants had mutations in all VInv alleles. Tubers from full VInv‐knockout plants had undetectable levels of reducing sugars, and processed chips contained reduced levels of acrylamide and were lightly coloured. Furthermore, seven of the 18 modified plant lines appeared to contain no TALEN DNA insertions in the potato genome. These results provide a framework for using TALENs to quickly improve traits in commercially relevant autotetraploid potato lines.
Bibliographie:	http://dx.doi.org/10.1111/pbi.12370 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1467-7644 1467-7652 1467-7652
DOI:	10.1111/pbi.12370