Generation of a recombinant Saffold Virus expressing UnaG as a marker for the visualization of viral infection

Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae , is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the u...

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Vydáno v:Virology journal Ročník 20; číslo 1; s. 175
Hlavní autoři: Okuwa, Takako, Himeda, Toshiki, Utani, Koichi, Higuchi, Masaya
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 07.08.2023
BioMed Central Ltd
Springer Nature B.V
BMC
Témata:
Amino acids
Analysis
Anguilla japonica
Antiviral agents
Antiviral drugs
Aseptic meningitis
Biomedical and Life Sciences
Biomedicine
Cardiovirus B
Care and treatment
Cloning
Control
Diagnosis
Diseases
Dosage and administration
family
Fluorescence
Gastrointestinal diseases
gastrointestinal system
Gene expression
Genes
genetic stability
Genomes
Genomics
genus
Green fluorescent protein
HeLa cells
High-throughput screening
Identification and classification
Infections
Meningitis
Methodology
Paralysis
Pathogenicity
Picornaviruses
Proteins
Saffold virus
Scientific equipment and supplies industry
UnaG
United States
viral genome
Viral infections
Viral proteins
Virology
Viruses
United States
Japan
Saffold virus
UnaG
ISSN:1743-422X, 1743-422X
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Shrnutí:Background Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae , is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV. Results We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or UnaG-expressing SAFV showed a bright green fluorescent signal, enabling convenient monitoring of SAFV infection. However, the expression of GFP but not UnaG was quickly lost during virus passaging due to the difference in genetic stability in the SAFV virus genome; the UnaG gene was stably maintained in the virus genome after at least five passages. Conclusions SAFV infection of cultured cells can easily be monitored using UnaG-expressing SAFV, which is superior to GFP in terms of genetic stability in the virus genome. This virus could be a useful tool for SAFV research, such as comparing the susceptibility of various cells to SAFV infection and evaluating the effects of antivirals on SAFV infection in high-throughput screening.
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ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-023-02142-8