A ML29 reassortant virus protects guinea pigs against a distantly related Nigerian strain of Lassa virus and can provide sterilizing immunity

Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced ste...

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Veröffentlicht in:Vaccine Jg. 25; H. 20; S. 4093 - 4102
Hauptverfasser: Carrion, Ricardo, Patterson, Jean L., Johnson, Curtis, Gonzales, Monica, Moreira, Carmen R., Ticer, Anysha, Brasky, Kathleen, Hubbard, Gene B., Moshkoff, Dmitry, Zapata, Juan, Salvato, Maria S., Lukashevich, Igor S.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Oxford Elsevier Ltd 16.05.2007
Elsevier
Elsevier Limited
Schlagworte:
Allergy and Immunology
Animals
Antibody Formation - immunology
Applied microbiology
Arenaviridae
Biological and medical sciences
Biomedical research
Cloning
Cross-protection
Disease
Experiments
Fatalities
Female
Fundamental and applied biological sciences. Psychology
Genetic diversity
Genomes
Guinea Pigs
Hemorrhage
Human viral diseases
Immunity, Cellular - immunology
Infections
Infectious diseases
Laboratories
Lassa fever
Lassa Fever - immunology
Lassa Fever - pathology
Lassa Fever - prevention & control
Lassa Fever - virology
Lassa fever and arenovirus diseases
Lassa virus
Lassa virus - immunology
Lassa virus - isolation & purification
Medical sciences
Microbiology
Miscellaneous
Nigeria
Proteins
Reassortant vaccine
Reassortant Viruses - genetics
Reassortant Viruses - immunology
RNA polymerase
Sterilizing immunity
Tropical viral diseases
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Viral diseases
Viral Vaccines - immunology
Viral Vaccines - pharmacology
Virology
Viruses
Nigeria
Guinea
Sierra Leone
Lassa fever
Cross-protection
Reassortant vaccine
Sterilizing immunity
Hemorrhagic fever
Genetic reassortment
Cross protection
Rodentia
Vaccine
Lassa virus
Arenavirus
Strain
Infection
Virus
Vertebrata
Mammalia
Guinea pig
Viral disease
Arbovirus disease
Arenaviridae
ISSN:0264-410X, 1873-2518
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Zusammenfassung:Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced sterilizing immunity and complete protection when challenged on day 30 either with homologous virus or with the distantly related Nigerian isolate. Simultaneous vaccination–challenge or challenge on day 2 after vaccination also protected 60–100% of the animals against both strains, but without sterilizing immunity. These results indicate that simultaneous replication of ML29 and LASV attenuates the virulence of LASV infection.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Corresponding author: Igor S. Lukashevich Institute of Human Virology University of Maryland Biotechnology Institute 725 West Lombard Street, Baltimore, MD 21201 TELE: 410-706-1366; FAX 410-706-5198; email: lukashev@umbi.umd.edu
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.02.038