Epitope-mapping of the glycoprotein from Crimean-Congo hemorrhagic fever virus using a microarray approach

Crimean-Congo hemorrhagic fever virus (CCHFV) causes severe acute human disease with lethal outcome. The knowledge about the immune response for this human health threat is highly limited. In this study, we have screened the glycoprotein of CCHFV for novel linear B-cell epitopic regions using a micr...

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Veröffentlicht in:PLoS neglected tropical diseases Jg. 12; H. 7; S. e0006598
Hauptverfasser: Fritzen, Amanda, Risinger, Christian, Korukluoglu, Gulay, Christova, Iva, Corli Hitzeroth, Arina, Viljoen, Natalie, Burt, Felicity Jane, Mirazimi, Ali, Blixt, Ola
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 09.07.2018
Public Library of Science (PLoS)
Schlagworte:
Amino acids
Analysis
Biology and Life Sciences
Care and treatment
Comparative genomic hybridization
Crimean hemorrhagic fever
Defence mechanisms
Diagnostic software
Diagnostic systems
Disease
Diseases
Ebola virus
Epitope mapping
Fever
Glycoproteins
Health aspects
Health risks
Health sciences
Hemorrhage
Human behavior
Human diseases
Immune response
Immune system
Immunity
Laboratories
Lymphocytes B
Medicine and health sciences
Microbiological strains
Mucin
People and Places
Peptides
Regions
Research and Analysis Methods
RNA polymerase
Samples
Sequencing
Specificity
Strains
Tropical diseases
Vaccines
Virology
Viruses
South Africa
Sweden
Turkey
Europe
Africa
Ankara Turkey
Denmark
ISSN:1935-2735, 1935-2727, 1935-2735
Online-Zugang:Volltext
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Zusammenfassung:Crimean-Congo hemorrhagic fever virus (CCHFV) causes severe acute human disease with lethal outcome. The knowledge about the immune response for this human health threat is highly limited. In this study, we have screened the glycoprotein of CCHFV for novel linear B-cell epitopic regions using a microarray approach. The peptide library consisted of 168 synthesized 20mer peptides with 10 amino acid overlap covering the entire glycoprotein. Using both pooled and individual human sera from survivors of CCHF disease in Turkey five peptide epitopes situated in the mucin-like region and GP 38 (G15-515) and GN G516-1037 region of the glycoprotein were identified as epitopes for a CCHF immune response. An epitope walk of the five peptides revealed a peptide sequence located in the GN region with high specificity and sensitivity. This peptide sequence, and a sequence downstream, reacted also against sera from survivors of CCHF disease in South Africa. The cross reactivity of these peptides with samples from a geographically distinct region where genetically diverse strains of the virus circulate, enabled the identification of unique peptide epitopes from the CCHF glycoprotein that could have application in development of diagnostic tools. In this study clinical samples from geographically distinct regions were used to identify conserved linear epitopic regions of the glycoprotein of CCHF.
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The authors have declared that no competing interests exist.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0006598